Detection of Intraplaque Hemorrhage in Mouse Atherosclerotic Lesions

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

Abstract

Intraplaque hemorrhage is defined as the presence of fresh or lysed erythrocytes, iron deposits in macrophages, and/or a fibrin clot in an atherosclerotic plaque. These features can be detected by hematoxylin and eosin, Martius scarlet Blue, and Perl's iron histological stainings. It is noteworthy that intraplaque hemorrhage is only present in murine atherosclerotic plaques after additional interventions or additional genetic traits affecting matrix degradation or thrombosis. In this chapter, we describe methods to detect intraplaque hemorrhage in mouse atherosclerotic lesions.

Original languageEnglish
Title of host publicationMethods in Mouse Atherosclerosis
EditorsVicente Andrés, Beatriz Dorado
Place of PublicationHumana New York
PublisherHumana New York
Pages339-348
Number of pages10
Edition1
ISBN (Electronic)978-1-4939-2929-0
ISBN (Print)978-1-4939-2928-3
DOIs
Publication statusPublished - 2015

Publication series

SeriesMethods in Molecular Biology
ISSN1064-3745

Keywords

  • Animals
  • Aorta
  • Aortic Diseases
  • Apolipoproteins E
  • Atherosclerosis
  • Azo Compounds
  • Coloring Agents
  • Disease Models, Animal
  • Eosine Yellowish-(YS)
  • Ferrocyanides
  • Genetic Predisposition to Disease
  • Hematoxylin
  • Hemorrhage
  • Mice
  • Mice, Knockout
  • Phenotype
  • Plaque, Atherosclerotic
  • Staining and Labeling

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