Abstract
Identifying the key toxic players within an in-vivo toxic syndrome is crucial to develop targeted therapies. Here, we established a novel method that characterizes the effect of single substances by means of an ex-vivo incubation set-up. We found that primary human spermatozoa elicit a distinct motile response on a (uremic) toxic milieu. Specifically, this approach describes the influence of a bulk toxic environment (uremia) as well as single substances (uremic toxins) by real-time analyzing motile cellular behavior. We established the human spermatozoa-based toxicity testing (HSTT) for detecting single substance-induced toxicity to be used as a screening tool to identify in-vivo toxins. Further, we propose an application of the HSTT as a method of clinical use to evaluate toxin-removing interventions (hemodialysis).
Original language | English |
---|---|
Article number | 14525 |
Number of pages | 11 |
Journal | Scientific Reports |
Volume | 9 |
DOIs | |
Publication status | Published - 10 Oct 2019 |
Keywords
- CHRONIC KIDNEY-DISEASE
- UREMIC TOXINS
- SPERM
- IDENTIFICATION
- MECHANISMS
- QUALITY
- PROTEIN