Inosine Triphosphate Pyrophosphohydrolase Expression: Decreased in Leukocytes of HIV-Infected Patients Using Combination Antiretroviral Therapy

N. Chantal Peltenburg; Mathie P. G. Leers; Jaap A. Bakker; Selwyn H. Lowe; Wim H. M. Vroemen; Aimee D. C. Paulussen; Bianca J. C. van den Bosch; Jorgen Bierau; Annelies Verbon

doi:10.1097/QAI.0000000000001130

Inosine Triphosphate Pyrophosphohydrolase Expression: Decreased in Leukocytes of HIV-Infected Patients Using Combination Antiretroviral Therapy

N. Chantal Peltenburg, Mathie P. G. Leers, Jaap A. Bakker, Selwyn H. Lowe, Wim H. M. Vroemen, Aimee D. C. Paulussen, Bianca J. C. van den Bosch, Jorgen Bierau, Annelies Verbon^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Objective: In HIV-infected patients, the enzyme Inosine triphosphate pyrophosphohydrolase (ITPase), involved in purine nucleotide homeostasis, was found to be decreased in erythrocytes. Since purine analogues are pivotal in the HIV treatment, a better understanding of ITPase expression in CD4(+) lymphocytes may lead to better understanding of nucleotide metabolism and (adverse) effects. Design: Cross-sectional, cohort, observational study. Methods: HIV-infected and control patients above 18 years were included. All DNA samples were genotyped for the 2 functional ITPA SNPs; c. 94C>A (rs1127354) and g. IVS+21A>C (rs7270101). ITPase expression was determined by flow cytometry in all leukocyte subsets. Results: Fifty-nine HIV-infected patients and 50 controls were included. Leukocyte subtype distribution showed no difference in monocytes and granulocytes, but lymphocytes were higher in HIV-infected patients (P

Original language	English
Pages (from-to)	390-395
Number of pages	6
Journal	Jaids-journal of Acquired Immune Deficiency Syndromes
Volume	73
Issue number	4
DOIs	https://doi.org/10.1097/QAI.0000000000001130
Publication status	Published - 1 Dec 2016

Keywords

ITPA
inosine triphosphatase
HIV
leukocytes
lymphocytes

Access to Document

10.1097/QAI.0000000000001130

Cite this

Peltenburg, N. C., Leers, M. P. G., Bakker, J. A., Lowe, S. H., Vroemen, W. H. M., Paulussen, A. D. C., van den Bosch, B. J. C., Bierau, J., & Verbon, A. (2016). Inosine Triphosphate Pyrophosphohydrolase Expression: Decreased in Leukocytes of HIV-Infected Patients Using Combination Antiretroviral Therapy. Jaids-journal of Acquired Immune Deficiency Syndromes, 73(4), 390-395. https://doi.org/10.1097/QAI.0000000000001130

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title = "Inosine Triphosphate Pyrophosphohydrolase Expression: Decreased in Leukocytes of HIV-Infected Patients Using Combination Antiretroviral Therapy",

abstract = "Objective: In HIV-infected patients, the enzyme Inosine triphosphate pyrophosphohydrolase (ITPase), involved in purine nucleotide homeostasis, was found to be decreased in erythrocytes. Since purine analogues are pivotal in the HIV treatment, a better understanding of ITPase expression in CD4(+) lymphocytes may lead to better understanding of nucleotide metabolism and (adverse) effects. Design: Cross-sectional, cohort, observational study. Methods: HIV-infected and control patients above 18 years were included. All DNA samples were genotyped for the 2 functional ITPA SNPs; c. 94C>A (rs1127354) and g. IVS+21A>C (rs7270101). ITPase expression was determined by flow cytometry in all leukocyte subsets. Results: Fifty-nine HIV-infected patients and 50 controls were included. Leukocyte subtype distribution showed no difference in monocytes and granulocytes, but lymphocytes were higher in HIV-infected patients (P",

keywords = "ITPA, inosine triphosphatase, HIV, leukocytes, lymphocytes",

author = "Peltenburg, {N. Chantal} and Leers, {Mathie P. G.} and Bakker, {Jaap A.} and Lowe, {Selwyn H.} and Vroemen, {Wim H. M.} and Paulussen, {Aimee D. C.} and {van den Bosch}, {Bianca J. C.} and Jorgen Bierau and Annelies Verbon",

year = "2016",

month = dec,

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doi = "10.1097/QAI.0000000000001130",

language = "English",

volume = "73",

pages = "390--395",

journal = "Jaids-journal of Acquired Immune Deficiency Syndromes",

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Peltenburg, NC, Leers, MPG, Bakker, JA, Lowe, SH, Vroemen, WHM, Paulussen, ADC , van den Bosch, BJC, Bierau, J & Verbon, A 2016, 'Inosine Triphosphate Pyrophosphohydrolase Expression: Decreased in Leukocytes of HIV-Infected Patients Using Combination Antiretroviral Therapy', Jaids-journal of Acquired Immune Deficiency Syndromes, vol. 73, no. 4, pp. 390-395. https://doi.org/10.1097/QAI.0000000000001130

Inosine Triphosphate Pyrophosphohydrolase Expression: Decreased in Leukocytes of HIV-Infected Patients Using Combination Antiretroviral Therapy. / Peltenburg, N. Chantal; Leers, Mathie P. G.; Bakker, Jaap A. et al.
In: Jaids-journal of Acquired Immune Deficiency Syndromes, Vol. 73, No. 4, 01.12.2016, p. 390-395.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Inosine Triphosphate Pyrophosphohydrolase Expression: Decreased in Leukocytes of HIV-Infected Patients Using Combination Antiretroviral Therapy

AU - Peltenburg, N. Chantal

AU - Leers, Mathie P. G.

AU - Bakker, Jaap A.

AU - Lowe, Selwyn H.

AU - Vroemen, Wim H. M.

AU - Paulussen, Aimee D. C.

AU - van den Bosch, Bianca J. C.

AU - Bierau, Jorgen

AU - Verbon, Annelies

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Objective: In HIV-infected patients, the enzyme Inosine triphosphate pyrophosphohydrolase (ITPase), involved in purine nucleotide homeostasis, was found to be decreased in erythrocytes. Since purine analogues are pivotal in the HIV treatment, a better understanding of ITPase expression in CD4(+) lymphocytes may lead to better understanding of nucleotide metabolism and (adverse) effects. Design: Cross-sectional, cohort, observational study. Methods: HIV-infected and control patients above 18 years were included. All DNA samples were genotyped for the 2 functional ITPA SNPs; c. 94C>A (rs1127354) and g. IVS+21A>C (rs7270101). ITPase expression was determined by flow cytometry in all leukocyte subsets. Results: Fifty-nine HIV-infected patients and 50 controls were included. Leukocyte subtype distribution showed no difference in monocytes and granulocytes, but lymphocytes were higher in HIV-infected patients (P

AB - Objective: In HIV-infected patients, the enzyme Inosine triphosphate pyrophosphohydrolase (ITPase), involved in purine nucleotide homeostasis, was found to be decreased in erythrocytes. Since purine analogues are pivotal in the HIV treatment, a better understanding of ITPase expression in CD4(+) lymphocytes may lead to better understanding of nucleotide metabolism and (adverse) effects. Design: Cross-sectional, cohort, observational study. Methods: HIV-infected and control patients above 18 years were included. All DNA samples were genotyped for the 2 functional ITPA SNPs; c. 94C>A (rs1127354) and g. IVS+21A>C (rs7270101). ITPase expression was determined by flow cytometry in all leukocyte subsets. Results: Fifty-nine HIV-infected patients and 50 controls were included. Leukocyte subtype distribution showed no difference in monocytes and granulocytes, but lymphocytes were higher in HIV-infected patients (P

KW - ITPA

KW - inosine triphosphatase

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KW - leukocytes

KW - lymphocytes

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SN - 1525-4135

VL - 73

SP - 390

EP - 395

JO - Jaids-journal of Acquired Immune Deficiency Syndromes

JF - Jaids-journal of Acquired Immune Deficiency Syndromes

IS - 4

ER -