Abstract
Objective: In HIV-infected patients, the enzyme Inosine triphosphate pyrophosphohydrolase (ITPase), involved in purine nucleotide homeostasis, was found to be decreased in erythrocytes. Since purine analogues are pivotal in the HIV treatment, a better understanding of ITPase expression in CD4(+) lymphocytes may lead to better understanding of nucleotide metabolism and (adverse) effects. Design: Cross-sectional, cohort, observational study. Methods: HIV-infected and control patients above 18 years were included. All DNA samples were genotyped for the 2 functional ITPA SNPs; c. 94C>A (rs1127354) and g. IVS+21A>C (rs7270101). ITPase expression was determined by flow cytometry in all leukocyte subsets. Results: Fifty-nine HIV-infected patients and 50 controls were included. Leukocyte subtype distribution showed no difference in monocytes and granulocytes, but lymphocytes were higher in HIV-infected patients (P
Original language | English |
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Pages (from-to) | 390-395 |
Number of pages | 6 |
Journal | JAIDS: journal of Acquired Immune Deficiency Syndromes |
Volume | 73 |
Issue number | 4 |
DOIs | |
Publication status | Published - 1 Dec 2016 |
Keywords
- ITPA
- inosine triphosphatase
- HIV
- leukocytes
- lymphocytes