TY - JOUR
T1 - Ultrahigh Sensitive Detection of Tau Protein as Alzheimer's Biomarker via Microfluidics and Nanofunctionalized Optical Fiber Sensors
AU - Chiavaioli, F.
AU - Rivero, D.S.
AU - Del Villar, I.
AU - Socorro-Leranoz, A.B.
AU - Zhang, X.J.
AU - Li, K.W.
AU - Santamaria, E.
AU - Fernandez-Irigoyen, J.
AU - Baldini, F.
AU - van den Hove, D.L.A.
AU - Shi, L.
AU - Bi, W.
AU - Guo, T.
AU - Giannetti, A.
AU - Matias, I.R.
PY - 2022/11/1
Y1 - 2022/11/1
N2 - Alzheimer's disease (AD) is one of the most common neurodegenerative illnesses displaying the highest death rate in the elderly. However, the existing AD diagnostic system remains elusive due to lack of a technology that may ensure enough sensitivity and reproducibility, detection accuracy, and specificity. Herein, a straightforward approach is reported to realize lab-on-fiber (LoF) technology for AD biomarker detection based on a D-shaped single-mode fiber combined with nanometer-scale metal-oxide film. The proposed sensing system, which permits the generation of lossy-mode resonance (LMR), remarkably increases the evanescent field of light guided through the fiber, and hence the fiber-surrounding medium interaction. Moreover, such optical sensors are highly repeatable in results and can safely be embedded into a compact and stable microfluidic system. Herein, the specific detection of Tau protein (as one of the classical AD biomarkers that is highly correlated with AD progression) in a complex biofluid with a detection limit of 10(-12) M and over a wide concentration range (10(-3)-10 mu gmL(-1)) is successfully demonstrated. The proposed LoF biosensor is an appealing solution for rapid, sub-microliter dose and highly sensitive detection of analytes at low concentrations, hereby having the potential toward early screening and personalized medicine in AD.
AB - Alzheimer's disease (AD) is one of the most common neurodegenerative illnesses displaying the highest death rate in the elderly. However, the existing AD diagnostic system remains elusive due to lack of a technology that may ensure enough sensitivity and reproducibility, detection accuracy, and specificity. Herein, a straightforward approach is reported to realize lab-on-fiber (LoF) technology for AD biomarker detection based on a D-shaped single-mode fiber combined with nanometer-scale metal-oxide film. The proposed sensing system, which permits the generation of lossy-mode resonance (LMR), remarkably increases the evanescent field of light guided through the fiber, and hence the fiber-surrounding medium interaction. Moreover, such optical sensors are highly repeatable in results and can safely be embedded into a compact and stable microfluidic system. Herein, the specific detection of Tau protein (as one of the classical AD biomarkers that is highly correlated with AD progression) in a complex biofluid with a detection limit of 10(-12) M and over a wide concentration range (10(-3)-10 mu gmL(-1)) is successfully demonstrated. The proposed LoF biosensor is an appealing solution for rapid, sub-microliter dose and highly sensitive detection of analytes at low concentrations, hereby having the potential toward early screening and personalized medicine in AD.
KW - Alzheimer's disease
KW - biophotonic platforms
KW - lossy-mode resonances
KW - microfluidics
KW - nanofunctionalized optical fibers
KW - SURFACE-PLASMON RESONANCE
KW - CEREBROSPINAL-FLUID
KW - MODE RESONANCE
KW - DISEASE
KW - DIAGNOSIS
KW - BLOOD
KW - CSF
KW - IMMUNOASSAY
KW - PROGRESSION
KW - MARKERS
U2 - 10.1002/adpr.202200044
DO - 10.1002/adpr.202200044
M3 - Article
SN - 2699-9293
VL - 3
JO - Advanced Photonics Research
JF - Advanced Photonics Research
IS - 11
M1 - 2200044
ER -