Translating myosin-binding protein C and titin abnormalities to whole-heart function using a novel calcium-contraction coupling model

Theo Arts*, Aurore Lyon, Tammo Delhaas, Diederik W D Kuster, Jolanda van der Velden, Joost Lumens

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Mutations in cardiac myosin-binding protein C (cMyBP-C) or titin may respectively lead to hypertrophic (HCM) or dilated (DCM) cardiomyopathies. The mechanisms leading to these phenotypes remain unclear because of the challenge of translating cellular abnormalities to whole-heart and system function. We developed and validated a novel computer model of calcium-contraction coupling incorporating the role of cMyBP-C and titin based on the key assumptions: 1) tension in the thick filament promotes cross-bridge attachment mechanochemically, 2) with increasing titin tension, more myosin heads are unlocked for attachment, and 3) cMyBP-C suppresses cross-bridge attachment. Simulated stationary calcium-tension curves, isotonic and isometric contractions, and quick release agreed with experimental data. The model predicted that a loss of cMyBP-C function decreases the steepness of the calcium-tension curve, and that more compliant titin decreases the level of passive and active tension and its dependency on sarcomere length. Integrating this cellular model in the CircAdapt model of the human heart and circulation showed that a loss of cMyBP-C function resulted in HCM-like hemodynamics with higher left ventricular end-diastolic pressures and smaller volumes. More compliant titin led to higher diastolic pressures and ventricular dilation, suggesting DCM-like hemodynamics. The novel model of calcium-contraction coupling incorporates the role of cMyBP-C and titin. Its coupling to whole-heart mechanics translates changes in cellular calcium-contraction coupling to changes in cardiac pump and circulatory function and identifies potential mechanisms by which cMyBP-C and titin abnormalities may develop into HCM and DCM phenotypes. This modeling platform may help identify distinct mechanisms underlying clinical phenotypes in cardiac diseases.
Original languageEnglish
Pages (from-to)13-23
Number of pages11
JournalJournal of Molecular and Cellular Cardiology
Volume190
Early online date8 Mar 2024
DOIs
Publication statusPublished - May 2024

Keywords

  • Calcium
  • Computer modeling
  • Mechanics
  • Sarcomere
  • Titin
  • cMyBP-C

Fingerprint

Dive into the research topics of 'Translating myosin-binding protein C and titin abnormalities to whole-heart function using a novel calcium-contraction coupling model'. Together they form a unique fingerprint.

Cite this