TY - JOUR
T1 - Tracking new insights into antifungal and anti-mycotoxigenic properties of a biofilm forming Pediococcus pentosaceus strain isolated from grain silage
AU - Mendonça, Carlos M.N.
AU - Oliveira, Rodrigo C.
AU - Pizauro, Lucas J.L.
AU - Pereira, Wellison A.
AU - Abboud, Kahlile
AU - Almeida, Sonia
AU - Watanabe, Ii Sei
AU - Varani, Alessandro M.
AU - Domínguez, José M.
AU - Correa, Benedito
AU - Venema, Koen
AU - Azevedo, Pamela O.S.
AU - Oliveira, Ricardo P.S.
N1 - Funding Information:
The authors are grateful to FAPESP (São Paulo Research Foundation) for processes n. 2018/25511-1 , n. 2020/13271-6 and n. 2021/15138-4 ; the National Council for Scientific and Technological Development—CNPq (processes No. 312923/2020-1 and 408783/2021-4 ); the Spanish Ministry of Science and Innovation (project PID2020-115879RB-I00 ); and Xunta de Galicia (Spain) since this study forms part of the activities of the Group with Potential for Growth ( GPC-ED431B 2021/23 ).
Publisher Copyright:
© 2023
PY - 2023/11/16
Y1 - 2023/11/16
N2 - The present study offers detailed insights into the antifungal and anti-mycotoxigenic potential of a biofilm forming lactic acid bacterium (Pediococcus pentosaceus) against one atoxigenic (Aspergillus flavus) and two toxigenic (Aspergillus nomius and Fusarium verticillioides) fungal strains. The antifungal effect of P. pentosaceus LBM18 strain was initially investigated through comparative analysis of fungi physiology by macroscopic visual evaluations and scanning electron microscopy examinations. The effects over fungal growth rate and asexual sporulation were additionally accessed. Furthermore, analytical evaluations of mycotoxin production were carried out by HPLC-MS/MS to provide insights on the bacterial anti-mycotoxigenic activity over fungal production of the aflatoxins B1, B2, G1 and G2 as well as fumonisins B1 and B2. Finally, reverse transcription quantitative real-time PCR (RT-qPCR) analysis was employed at the most effective bacterial inoculant concentration to evaluate, at the molecular level, the down-regulation of genes aflR, aflQ and aflD, related to the biosynthesis of aflatoxins by the strain of Aspergillus nomius. The effects over mycotoxin contamination were thought to be result of a combination of several biotic and abiotic factors, such as interaction between living beings and physical-chemical aspects of the environment, respectively. Several possible mechanisms of action were addressed along with potentially deleterious effects ascribing from P. pentosaceus misuse as biopesticide, emphasizing the importance of evaluating lactic acid bacteria safety in new applications, concentrations, and exposure scenarios.
AB - The present study offers detailed insights into the antifungal and anti-mycotoxigenic potential of a biofilm forming lactic acid bacterium (Pediococcus pentosaceus) against one atoxigenic (Aspergillus flavus) and two toxigenic (Aspergillus nomius and Fusarium verticillioides) fungal strains. The antifungal effect of P. pentosaceus LBM18 strain was initially investigated through comparative analysis of fungi physiology by macroscopic visual evaluations and scanning electron microscopy examinations. The effects over fungal growth rate and asexual sporulation were additionally accessed. Furthermore, analytical evaluations of mycotoxin production were carried out by HPLC-MS/MS to provide insights on the bacterial anti-mycotoxigenic activity over fungal production of the aflatoxins B1, B2, G1 and G2 as well as fumonisins B1 and B2. Finally, reverse transcription quantitative real-time PCR (RT-qPCR) analysis was employed at the most effective bacterial inoculant concentration to evaluate, at the molecular level, the down-regulation of genes aflR, aflQ and aflD, related to the biosynthesis of aflatoxins by the strain of Aspergillus nomius. The effects over mycotoxin contamination were thought to be result of a combination of several biotic and abiotic factors, such as interaction between living beings and physical-chemical aspects of the environment, respectively. Several possible mechanisms of action were addressed along with potentially deleterious effects ascribing from P. pentosaceus misuse as biopesticide, emphasizing the importance of evaluating lactic acid bacteria safety in new applications, concentrations, and exposure scenarios.
KW - Aflatoxins
KW - Biofilm
KW - Crops contamination
KW - Fumonisin
KW - Lactic acid bacteria
KW - Mycotoxin
UR - http://www.scopus.com/inward/record.url?scp=85165662212&partnerID=8YFLogxK
U2 - 10.1016/j.ijfoodmicro.2023.110337
DO - 10.1016/j.ijfoodmicro.2023.110337
M3 - Article
C2 - 37506547
SN - 0168-1605
VL - 405
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
M1 - 110337
ER -