The use of a test battery assessing affective behavior in rats: Order effects

A. Blokland, S. ten Oever, D. van Gorp, M. van Draanen, T. Schmidt, E. Nguyen, A. Krugliak, A. Napoletano, S. Keuter, I. Klinkenberg

Research output: Contribution to journalArticleAcademicpeer-review

20 Citations (Scopus)

Abstract

Many studies have used test batteries for the evaluation of affective behavior in rodents. This has the advantage that treatment effects can be examined on different aspects of the affective domain. However, the behavior in one test may affect the behavior in following test. The present study examined possible order effects in rats that were tested in three different tests: Open Field (OF), Zero Maze (ZM) and Forced Swim Test (FST). The data of the present study indicated that the behavior in ZM was the least affected by the order of testing. In contrast, the behavior in the FST (and to a less extend the OF) was dependent on the order of the test in the test battery. Repeated testing in the same test did not change the behavior in the ZM. However, the behavior in the OF and FST changed with repeated testing. The present study indicates that the performance of rats in a test can be dependent on the order in a test battery. Consequently, these data caution the interpretation of treatment effects in studies in which test batteries are used.
Original languageEnglish
Pages (from-to)16-21
Number of pages6
JournalBehavioural Brain Research
Volume228
Issue number1
DOIs
Publication statusPublished - 1 Mar 2012

Keywords

  • Anxiety
  • Depression
  • Animal model
  • Order effect
  • Forced Swim Test
  • Open Field
  • Zero Maze
  • ELEVATED PLUS-MAZE
  • ANIMAL-MODEL
  • OPEN-FIELD
  • CIRCADIAN PHASE
  • ZERO-MAZE
  • ANXIETY
  • DEPRESSION
  • STRAINS
  • MICE
  • ILLUMINATION

Cite this

Blokland, A. ; ten Oever, S. ; van Gorp, D. ; van Draanen, M. ; Schmidt, T. ; Nguyen, E. ; Krugliak, A. ; Napoletano, A. ; Keuter, S. ; Klinkenberg, I. / The use of a test battery assessing affective behavior in rats: Order effects. In: Behavioural Brain Research. 2012 ; Vol. 228, No. 1. pp. 16-21.
@article{1a24bf77a0ee4868b684da6445d9fcc0,
title = "The use of a test battery assessing affective behavior in rats: Order effects",
abstract = "Many studies have used test batteries for the evaluation of affective behavior in rodents. This has the advantage that treatment effects can be examined on different aspects of the affective domain. However, the behavior in one test may affect the behavior in following test. The present study examined possible order effects in rats that were tested in three different tests: Open Field (OF), Zero Maze (ZM) and Forced Swim Test (FST). The data of the present study indicated that the behavior in ZM was the least affected by the order of testing. In contrast, the behavior in the FST (and to a less extend the OF) was dependent on the order of the test in the test battery. Repeated testing in the same test did not change the behavior in the ZM. However, the behavior in the OF and FST changed with repeated testing. The present study indicates that the performance of rats in a test can be dependent on the order in a test battery. Consequently, these data caution the interpretation of treatment effects in studies in which test batteries are used.",
keywords = "Anxiety, Depression, Animal model, Order effect, Forced Swim Test, Open Field, Zero Maze, ELEVATED PLUS-MAZE, ANIMAL-MODEL, OPEN-FIELD, CIRCADIAN PHASE, ZERO-MAZE, ANXIETY, DEPRESSION, STRAINS, MICE, ILLUMINATION",
author = "A. Blokland and {ten Oever}, S. and {van Gorp}, D. and {van Draanen}, M. and T. Schmidt and E. Nguyen and A. Krugliak and A. Napoletano and S. Keuter and I. Klinkenberg",
year = "2012",
month = "3",
day = "1",
doi = "10.1016/j.bbr.2011.11.042",
language = "English",
volume = "228",
pages = "16--21",
journal = "Behavioural Brain Research",
issn = "0166-4328",
publisher = "Elsevier Science",
number = "1",

}

Blokland, A, ten Oever, S, van Gorp, D, van Draanen, M, Schmidt, T, Nguyen, E, Krugliak, A, Napoletano, A, Keuter, S & Klinkenberg, I 2012, 'The use of a test battery assessing affective behavior in rats: Order effects', Behavioural Brain Research, vol. 228, no. 1, pp. 16-21. https://doi.org/10.1016/j.bbr.2011.11.042

The use of a test battery assessing affective behavior in rats: Order effects. / Blokland, A.; ten Oever, S.; van Gorp, D.; van Draanen, M.; Schmidt, T.; Nguyen, E.; Krugliak, A.; Napoletano, A.; Keuter, S.; Klinkenberg, I.

In: Behavioural Brain Research, Vol. 228, No. 1, 01.03.2012, p. 16-21.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The use of a test battery assessing affective behavior in rats: Order effects

AU - Blokland, A.

AU - ten Oever, S.

AU - van Gorp, D.

AU - van Draanen, M.

AU - Schmidt, T.

AU - Nguyen, E.

AU - Krugliak, A.

AU - Napoletano, A.

AU - Keuter, S.

AU - Klinkenberg, I.

PY - 2012/3/1

Y1 - 2012/3/1

N2 - Many studies have used test batteries for the evaluation of affective behavior in rodents. This has the advantage that treatment effects can be examined on different aspects of the affective domain. However, the behavior in one test may affect the behavior in following test. The present study examined possible order effects in rats that were tested in three different tests: Open Field (OF), Zero Maze (ZM) and Forced Swim Test (FST). The data of the present study indicated that the behavior in ZM was the least affected by the order of testing. In contrast, the behavior in the FST (and to a less extend the OF) was dependent on the order of the test in the test battery. Repeated testing in the same test did not change the behavior in the ZM. However, the behavior in the OF and FST changed with repeated testing. The present study indicates that the performance of rats in a test can be dependent on the order in a test battery. Consequently, these data caution the interpretation of treatment effects in studies in which test batteries are used.

AB - Many studies have used test batteries for the evaluation of affective behavior in rodents. This has the advantage that treatment effects can be examined on different aspects of the affective domain. However, the behavior in one test may affect the behavior in following test. The present study examined possible order effects in rats that were tested in three different tests: Open Field (OF), Zero Maze (ZM) and Forced Swim Test (FST). The data of the present study indicated that the behavior in ZM was the least affected by the order of testing. In contrast, the behavior in the FST (and to a less extend the OF) was dependent on the order of the test in the test battery. Repeated testing in the same test did not change the behavior in the ZM. However, the behavior in the OF and FST changed with repeated testing. The present study indicates that the performance of rats in a test can be dependent on the order in a test battery. Consequently, these data caution the interpretation of treatment effects in studies in which test batteries are used.

KW - Anxiety

KW - Depression

KW - Animal model

KW - Order effect

KW - Forced Swim Test

KW - Open Field

KW - Zero Maze

KW - ELEVATED PLUS-MAZE

KW - ANIMAL-MODEL

KW - OPEN-FIELD

KW - CIRCADIAN PHASE

KW - ZERO-MAZE

KW - ANXIETY

KW - DEPRESSION

KW - STRAINS

KW - MICE

KW - ILLUMINATION

U2 - 10.1016/j.bbr.2011.11.042

DO - 10.1016/j.bbr.2011.11.042

M3 - Article

VL - 228

SP - 16

EP - 21

JO - Behavioural Brain Research

JF - Behavioural Brain Research

SN - 0166-4328

IS - 1

ER -