Abstract
In tissue engineering, cell origin is important to ensure outcome quality. However, the impact of the cell type chosen for seeding in a biocompatible matrix has been less investigated. Here, we investigated the capacity of primary and immortalized fibroblasts of distinct origins to degrade a gelatin/alginate/fibrin (GAF)-based biomaterial. We further established that fibrin was targeted by degradative fibroblasts through the secretion of fibrinolytic matrix-metalloproteinases (MMPs) and urokinase, two types of serine protease. Finally, we demonstrated that besides aprotinin, specific targeting of fibrinolytic MMPs and urokinase led to cell-laden GAF stability for at least forty-eight hours. These results support the use of specific strategies to tune fibrin-based biomaterials degradation over time. It emphasizes the need to choose the right cell type and further bring targeted solutions to avoid the degradation of fibrin-containing hydrogels or bioinks.
Original language | English |
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Article number | 920929 |
Number of pages | 12 |
Journal | Frontiers in bioengineering and biotechnology |
Volume | 10 |
DOIs | |
Publication status | Published - 22 Jul 2022 |
Keywords
- APROTININ
- DIFFERENTIATION
- EXTRACELLULAR-MATRIX
- FIBROBLASTS
- MECHANICAL-PROPERTIES
- ORAL-MUCOSA
- SCAFFOLDS
- SKIN
- SYSTEM
- TISSUE CONSTRUCTS
- aprotinin
- connective tissue
- fibrinolysis
- fibroblasts
- gelatin
- matrix metalloproteinases
- sodium alginate
- tissue engineering