The arrhythmogenic cardiomyopathy phenotype associated with PKP2 c.1211dup variant

Thomas A. Bos, Sebastiaan R.D. Piers, Marja W. Wessels, Arjan C. Houweling, Regina Bökenkamp, Marianne Bootsma, Laurens P. Bosman, Reinder Evertz, Debby M.E.I. Hellebrekers, Yvonne M. Hoedemaekers, Jeroen Knijnenburg, Ronald Lekanne Deprez, Anneke M. van Mil, Anneline S.J.M. te Riele, Marjon A. van Slegtenhorst, Arthur A.M. Wilde, Sing Chien Yap, Dennis Dooijes, Tamara T. Koopmann, J. Peter van TintelenDaniela Q.C.M. Barge-Schaapveld*, European Reference Network for rare, low prevalence and complex diseases of the heart: ERN GUARD-Heart

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background: The arrhythmogenic cardiomyopathy (ACM) phenotype, with life-threatening ventricular arrhythmias and heart failure, varies according to genetic aetiology. We aimed to characterise the phenotype associated with the variant c.1211dup (p.Val406Serfs*4) in the plakophilin‑2 gene (PKP2) and compare it with previously reported Dutch PKP2 founder variants. Methods: Clinical data were collected retrospectively from medical records of 106 PKP2 c.1211dup heterozygous carriers. Using data from the Netherlands ACM Registry, c.1211dup was compared with 3 other truncating PKP2 variants (c.235C > T (p.Arg79*), c.397C > T (p.Gln133*) and c.2489+1G > A (p.?)). Results: Of the 106 carriers, 47 (44%) were diagnosed with ACM, at a mean age of 41 years. By the end of follow-up, 29 (27%) had experienced sustained ventricular arrhythmias and 12 (11%) had developed heart failure, with male carriers showing significantly higher risks than females on these endpoints (p < 0.05). Based on available cardiac magnetic resonance imaging and echocardiographic data, 46% of the carriers showed either right ventricular dilatation and/or dysfunction, whereas a substantial minority (37%) had some form of left ventricular involvement. Both geographical distribution of carriers and haplotype analysis suggested PKP2 c.1211dup to be a founder variant originating from the South-Western coast of the Netherlands. Finally, a Cox proportional hazards model suggested significant differences in ventricular arrhythmia–free survival between 4 PKP2 founder variants, including c.1211dup. Conclusions: The PKP2 c.1211dup variant is a Dutch founder variant associated with a typical right-dominant ACM phenotype, but also left ventricular involvement, and a possibly more severe phenotype than other Dutch PKP2 founder variants.

Original languageEnglish
Pages (from-to)315-323
Number of pages9
JournalNetherlands Heart Journal
Volume31
Issue number7-8
DOIs
Publication statusPublished - Aug 2023

Keywords

  • Arrhythmogenic Cardiomyopathy
  • Founder mutation
  • Genetics
  • Plakophilin-2

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