@article{222d827823de45b383eb14627d3efa2d,
title = "Targeting a coatomer protein complex-I gene via RNA interference results in effective lethality in the pollen beetle Brassicogethes aeneus",
abstract = "The pollen beetle Brassicogethes aeneus is a serious pest of oilseed rape (Brassica napus) in Europe. Management of this pest has grown difficult due to B. aeneus's development of resistance to pyrethroid insecticides, as well as the pressure to establish control strategies that minimise the impact on nontarget organisms. RNA interference represents a nucleotide sequence-based, and thus potentially species-specific, approach to agricultural pest control. The present study examined the efficacy of targeting the coatomer gene coatomer subunit alpha (alpha COP), via both microinjection and dietary exposure to exogenous complementary dsRNA, on alpha COP-silencing and subsequent mortality in B. aeneus. Beetles injected with dsRNA targeting alpha COP (at 0.14 mu g/mg) showed 88% and 100% mortality at 6 and 10 days post-injection, respectively; where by the same time after dietary exposure, 43%-89% mortality was observed in the 3 mu g dsRNA/mu L treatment, though the effect was concentration-dependent. Thus, the effect was significant for both delivery routes. In working towards RNA-based management of B. aeneus, future studies should include alpha COP as a target of interest.",
keywords = "RNAi, Brassicogethes aeneus, Meligethes aeneus, Oilseed rape, Rapeseed, COPI, Biopesticide, Coleoptera, INSECTICIDES, POPULATIONS, MECHANISMS, RESISTANCE, WEEVIL, LARVAL, SYSTEM, DSRNA, PESTS",
author = "Jonathan Willow and Silva Sulg and Taning, {Clauvis Nji Tizi} and Silva, {Ana Isabel} and Olivier Christiaens and Riina Kaasik and Katterinne Prentice and Lovei, {Gabor L.} and Guy Smagghe and Eve Veromann",
note = "Funding Information: We thank the owners of the land on which pollen beetles were collected for this study, and a member of the Estonian University of Life Sciences{\textquoteright} Chair of Plant Breeding and Plant Biology for molecular support. Jonathan Willow is the recipient of a PhD grant from the European Social Fund for Doctoral Students, as well as the Internationalisation Programme DoRa (carried out by the Archimedes Foundation). Olivier Christiaens is a recipient of a postdoctoral fellowship from the Research Foundation—Flanders (FWO-Vlaanderen). The authors acknowledge financial support from the institutional Research Funding project no IUT36-2 of the Estonian Research Council, the European ERA-NET C-IPM project “IPM4Meligethes” (Project No 3G0H0416), the Special Research Fund (BOF) of Ghent University and the Research Foundation—Flanders (FWO-Vlaanderen). Funding Information: We thank the owners of the land on which pollen beetles were collected for this study, and a member of the Estonian University of Life Sciences? Chair of Plant Breeding and Plant Biology for molecular support. Jonathan Willow is the recipient of a PhD grant from the European Social Fund for Doctoral Students, as well as the Internationalisation Programme DoRa (carried out by the Archimedes Foundation). Olivier Christiaens is a recipient of a postdoctoral fellowship from the Research Foundation?Flanders (FWO-Vlaanderen). The authors acknowledge financial support from the institutional Research Funding project no IUT36-2 of the Estonian Research Council, the European ERA-NET C-IPM project ?IPM4Meligethes? (Project No 3G0H0416), the Special Research Fund (BOF) of Ghent University and the Research Foundation?Flanders (FWO-Vlaanderen). Funding Information: This study was partially funded by the institutional Research Funding project no IUT36-2 of the Estonian Research Council, the European ERA-NET C-IPM project “IPM4Meligethes” (Project No 3G0H0416), the Special Research Fund (BOF) of Ghent University and the Research Foundation—Flanders (FWO-Vlaanderen). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Acknowledgements Publisher Copyright: {\textcopyright} 2020, The Author(s).",
year = "2021",
month = jun,
doi = "10.1007/s10340-020-01288-6",
language = "English",
volume = "94",
pages = "703--712",
journal = "Journal of Pest Science",
issn = "1612-4758",
publisher = "Springer Verlag",
number = "3",
}