Systemic and Pulmonary Oxidative Stress After Single-Leg Exercise in COPD

E.M. Mercken*, H.R. Gosker, E.P. Rutten, E.F. Wouters, A. Bast, G.J. Hageman, A.M. Schols

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    108 Downloads (Pure)


    BACKGROUND: The aim of this study was to disentangle the contribution of muscular vs pulmonary oxidative stress during endurance exercise in patients with COPD. METHODS: Fifteen COPD patients and 10 healthy age-matched controls performed a continuously submaximal single leg ergometer test (40% of peak workload) for 20 min or until they stopped (Tlim). Venous blood, urine samples and exhaled breath condensate were sampled before, immediately and 2h after exercise. RESULTS: Tlim was lower in COPD than controls (p < 0.01). No exercise-induced systemic inflammation (IL-6, TNF-alpha) was found in the groups. Urinary malondialdehyde (MDA) and uric acid (p < 0.05) were increased in COPD whereas erythrocyte GSSG/GSH tended to be increased in COPD compared to controls after exercise (p = 0.08). Despite the relatively low cardioventilatory response to this localized muscle exercise, hydrogen peroxide (H(2)O(2)) in breath condensate significantly increased in COPD (p < 0.01). NF-kappaB DNA binding activity of p50 in peripheral blood monocytes was elevated after exercise in both COPD (p < 0.01) and controls (p < 0.05), whereas p65 protein was not altered. CONCLUSION: COPD patients showed increased pulmonary and systemic oxidative stress after localized leg muscle exercise compared to healthy controls without evidence of increased levels of systemic inflammation.
    Original languageEnglish
    Pages (from-to)1291-1300
    Issue number5
    Publication statusPublished - 1 Jan 2009


    Dive into the research topics of 'Systemic and Pulmonary Oxidative Stress After Single-Leg Exercise in COPD'. Together they form a unique fingerprint.

    Cite this