Steep increase in myonuclear domain size during infancy

T. Delhaas, S. F. Van der Meer, G. Schaart, H. Degens, M.R. Drost

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

We investigated whether myonuclear number increases in proportion to the increase in fiber size during maturational growth of skeletal muscle. Thoraco-abdominal muscle tissue was obtained from twenty 6-day to 15-year-old boys and girls during cardiothoracic surgery. Cross-sections were stained by anti-laminin for the basal lamina and by DAPI to identify nuclei. Basal lamina was traced on digital images to measure the fiber cross-sectional area (FCSA). Nuclei located within the basal lamina were considered myonuclei if pax7-negative and satellite cell nuclei if pax7-positive. Samples of two children were excluded from analysis because of clear signs of hypoxia as shown by positive carbonic anhydrase IX staining. Linear regression showed that FCSA increased with age by 187 mum(2) .per annum (R(2) = 0.90; P < 0.001). Satellite cell density showed a dramatic decrease in the first months of life, but this was not accompanied by an increase in myonuclei per muscle fiber cross-section. Till four years of age the number of myonuclei per muscle fiber cross-section remained relatively constant but increased thereafter. Myonuclear domain size showed a steep increase during infancy and reached adult values in the young adolescent phase. Anat Rec, 2013. (c) 2012 Wiley Periodicals, Inc.
Original languageEnglish
Pages (from-to)192-197
Number of pages6
JournalThe Anatomical Record: advances in integrative anatomy and evolutionary biology
Volume296
Issue number2
DOIs
Publication statusPublished - Feb 2013

Keywords

  • skeletal muscle
  • myonuclei
  • satellite cell
  • myonuclear domain
  • muscle fiber size
  • maturation
  • SKELETAL-MUSCLE
  • SATELLITE CELLS
  • FIBER HYPERPLASIA
  • MYOFIBER GROWTH
  • RAT SOLEUS
  • NUMBER
  • YOUNG
  • MEN
  • NUCLEI
  • WOMEN

Cite this

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title = "Steep increase in myonuclear domain size during infancy",
abstract = "We investigated whether myonuclear number increases in proportion to the increase in fiber size during maturational growth of skeletal muscle. Thoraco-abdominal muscle tissue was obtained from twenty 6-day to 15-year-old boys and girls during cardiothoracic surgery. Cross-sections were stained by anti-laminin for the basal lamina and by DAPI to identify nuclei. Basal lamina was traced on digital images to measure the fiber cross-sectional area (FCSA). Nuclei located within the basal lamina were considered myonuclei if pax7-negative and satellite cell nuclei if pax7-positive. Samples of two children were excluded from analysis because of clear signs of hypoxia as shown by positive carbonic anhydrase IX staining. Linear regression showed that FCSA increased with age by 187 mum(2) .per annum (R(2) = 0.90; P < 0.001). Satellite cell density showed a dramatic decrease in the first months of life, but this was not accompanied by an increase in myonuclei per muscle fiber cross-section. Till four years of age the number of myonuclei per muscle fiber cross-section remained relatively constant but increased thereafter. Myonuclear domain size showed a steep increase during infancy and reached adult values in the young adolescent phase. Anat Rec, 2013. (c) 2012 Wiley Periodicals, Inc.",
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author = "T. Delhaas and {Van der Meer}, {S. F.} and G. Schaart and H. Degens and M.R. Drost",
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language = "English",
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Steep increase in myonuclear domain size during infancy. / Delhaas, T.; Van der Meer, S. F.; Schaart, G.; Degens, H.; Drost, M.R.

In: The Anatomical Record: advances in integrative anatomy and evolutionary biology, Vol. 296, No. 2, 02.2013, p. 192-197.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Steep increase in myonuclear domain size during infancy

AU - Delhaas, T.

AU - Van der Meer, S. F.

AU - Schaart, G.

AU - Degens, H.

AU - Drost, M.R.

PY - 2013/2

Y1 - 2013/2

N2 - We investigated whether myonuclear number increases in proportion to the increase in fiber size during maturational growth of skeletal muscle. Thoraco-abdominal muscle tissue was obtained from twenty 6-day to 15-year-old boys and girls during cardiothoracic surgery. Cross-sections were stained by anti-laminin for the basal lamina and by DAPI to identify nuclei. Basal lamina was traced on digital images to measure the fiber cross-sectional area (FCSA). Nuclei located within the basal lamina were considered myonuclei if pax7-negative and satellite cell nuclei if pax7-positive. Samples of two children were excluded from analysis because of clear signs of hypoxia as shown by positive carbonic anhydrase IX staining. Linear regression showed that FCSA increased with age by 187 mum(2) .per annum (R(2) = 0.90; P < 0.001). Satellite cell density showed a dramatic decrease in the first months of life, but this was not accompanied by an increase in myonuclei per muscle fiber cross-section. Till four years of age the number of myonuclei per muscle fiber cross-section remained relatively constant but increased thereafter. Myonuclear domain size showed a steep increase during infancy and reached adult values in the young adolescent phase. Anat Rec, 2013. (c) 2012 Wiley Periodicals, Inc.

AB - We investigated whether myonuclear number increases in proportion to the increase in fiber size during maturational growth of skeletal muscle. Thoraco-abdominal muscle tissue was obtained from twenty 6-day to 15-year-old boys and girls during cardiothoracic surgery. Cross-sections were stained by anti-laminin for the basal lamina and by DAPI to identify nuclei. Basal lamina was traced on digital images to measure the fiber cross-sectional area (FCSA). Nuclei located within the basal lamina were considered myonuclei if pax7-negative and satellite cell nuclei if pax7-positive. Samples of two children were excluded from analysis because of clear signs of hypoxia as shown by positive carbonic anhydrase IX staining. Linear regression showed that FCSA increased with age by 187 mum(2) .per annum (R(2) = 0.90; P < 0.001). Satellite cell density showed a dramatic decrease in the first months of life, but this was not accompanied by an increase in myonuclei per muscle fiber cross-section. Till four years of age the number of myonuclei per muscle fiber cross-section remained relatively constant but increased thereafter. Myonuclear domain size showed a steep increase during infancy and reached adult values in the young adolescent phase. Anat Rec, 2013. (c) 2012 Wiley Periodicals, Inc.

KW - skeletal muscle

KW - myonuclei

KW - satellite cell

KW - myonuclear domain

KW - muscle fiber size

KW - maturation

KW - SKELETAL-MUSCLE

KW - SATELLITE CELLS

KW - FIBER HYPERPLASIA

KW - MYOFIBER GROWTH

KW - RAT SOLEUS

KW - NUMBER

KW - YOUNG

KW - MEN

KW - NUCLEI

KW - WOMEN

U2 - 10.1002/ar.22631

DO - 10.1002/ar.22631

M3 - Article

VL - 296

SP - 192

EP - 197

JO - The Anatomical Record: advances in integrative anatomy and evolutionary biology

JF - The Anatomical Record: advances in integrative anatomy and evolutionary biology

SN - 1932-8486

IS - 2

ER -