Detection of antibodies using immunofluoresence tests (IFAT) is recommended for diagnosis of chronic Q fever, but other commercial antibody assays are also available. We compared an enzyme-linked immunosorbent assay (ELISA) (Virion/Serion) and a complement fixation test (CFT) (Virion/Serion) for the detection of Coxiella burnetii IgG phase I and IgA phase I in early- and follow-up serum samples from patients with chronic Q fever, diagnosed according to an algorithm that involves IFAT. For this, we tested sera of 49 patients, including 30 proven, 14 probable and five possible chronic Q fever cases. Sensitivity of CFT for diagnosis of chronic Q fever was suboptimal (85 %), as eight patients, including five with chronic Q fever, tested negative at time of diagnosis, whereas IgG phase I antibodies were detected in these five patients by ELISA. Sensitivity of ELISA was higher, although three probable patients were missed. No differences in ELISA IgA phase I detection between proven chronic Q fever and probable were observed; instead possible patients were in majority IgA negative (60 %). Serological examination using ELISA and CFT in follow-up sera from 26 patients on treatment was unsatisfactory. Like IFAT, all kinetic options were possible: decreasing, remaining stable or even increase during time. This study demonstrated that the sensitivity of CFT-based phase I antibody detection is low and therefore not recommended for diagnosis of chronic Q fever. Based on our results, serological follow-up to guide treatment decisions was of limited value.
|Number of pages||6|
|Journal||European Journal of Clinical Microbiology & Infectious Diseases|
|Publication status||Published - Jul 2014|
Wegdam-Blans, M. C. A., Tjhie, H. T., Korbeeck, J. M., Nabuurs-Franssen, M. N., Kampschreur, L. M., Sprong, T., Teijink, J. A. W., & Koopmans, M. P. (2014). Serology in chronic Q fever is still surrounded by question marks. European Journal of Clinical Microbiology & Infectious Diseases, 33(7), 1089-1094. https://doi.org/10.1007/s10096-014-2048-4