Scavenger receptor collectin placenta 1 is a novel receptor involved in the uptake of myelin by phagocytes

Jeroen F. J. Bogie, Jo Mailleux, Elien Wouters, Winde Jorissen, Elien Grajchen, Jasmine Vanmol, Kristiaan Wouters, Niels Hellings, Jack Van Horsen, Tim Vanmierlo, Jerome J. A. Hendriks

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Myelin-containing macrophages and microglia are the most abundant immune cells in active multiple sclerosis (MS) lesions. Our recent transcriptomic analysis demonstrated that collectin placenta 1 (CL-P1) is one of the most potently induced genes in macrophages after uptake of myelin. CL-P1 is a type II transmembrane protein with both a collagen-like and carbohydrate recognition domain, which plays a key role in host defense. In this study we sought to determine the dynamics of CL-P1 expression on myelin-containing phagocytes and define the role that it plays in MS lesion development. We show that myelin uptake increases the cell surface expression of CL-P1 by mouse and human macrophages, but not by primary mouse microglia in vitro. In active demyelinating MS lesions, CL-P1 immunoreactivity was localized to perivascular and parenchymal myelin-laden phagocytes. Finally, we demonstrate that CL-P1 is involved in myelin internalization as knockdown of CL-P1 markedly reduced myelin uptake. Collectively, our data indicate that CL-P1 is a novel receptor involved in myelin uptake by phagocytes and likely plays a role in MS lesion development.

Original languageEnglish
Article number44794
Number of pages9
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 20 Mar 2017

Keywords

  • Journal Article

Research Output

  • 1 Erratum / corrigendum

Corrigendum: Scavenger receptor collectin placenta 1 is a novel receptor involved in the uptake of myelin by phagocytes

Bogie, J. F. J., Mailleux, J., Wouters, E., Jorissen, W., Grajchen, E., Vanmol, J., Wouters, K., Hellings, N., van Horssen, J., Vanmierlo, T. & Hendriks, J. J. A., 22 Dec 2017, In : Scientific Reports. 7, 1 p., 46925.

Research output: Contribution to journalErratum / corrigendumAcademic

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Cite this

Bogie, J. F. J., Mailleux, J., Wouters, E., Jorissen, W., Grajchen, E., Vanmol, J., Wouters, K., Hellings, N., Van Horsen, J., Vanmierlo, T., & Hendriks, J. J. A. (2017). Scavenger receptor collectin placenta 1 is a novel receptor involved in the uptake of myelin by phagocytes. Scientific Reports, 7, [44794]. https://doi.org/10.1038/srep44794