TY - JOUR
T1 - Role of Endocannabinoids in Energy-Balance Regulation in Participants in the Postobese State-a PREVIEW Study
AU - Drummen, Mathijs
AU - Tischmann, Lea
AU - Gatta-Cherifi, Blandine
AU - Cota, Daniela
AU - Matias, Isabelle
AU - Raben, Anne
AU - Adam, Tanja
AU - Westerterp-Plantenga, Margriet
N1 - Funding Information:
This work was supported by the EU 7th Framework Programme (Grant 312057, FP7-KBBE-2012.2.2-03); the Dutch Organization for Scientific Research (NWO ASPASIA Grant 015.010.034); and INSERM (to D.C. and I.M.). The analytical chemistry platform at Neurocentre Magendie is supported by INSERM and Labex BRAIN ANR-10-LABX-43.
Publisher Copyright:
© 2020 Endocrine Society. All rights reserved.
PY - 2020/7
Y1 - 2020/7
N2 - Context: Endocannabinoids are suggested to play a role in energy balance regulation.Objective: We aimed to investigate associations of endocannabinoid concentrations during the day with energy balance and adiposity and interactions with 2 diets differing in protein content in participants in the postobese phase with prediabetes.Design and Participants: Participants (n = 38) were individually fed in energy balance with a medium protein (MP: 15:55:30% of energy from protein:carbohydrate:fat) or high-protein diet (HP: 25:45:30% energy from P:C:F) for 48 hours in a respiration chamber.Main Outcome Measures: Associations between energy balance, energy expenditure, respiratory quotient, and endocannabinoid concentrations during the day were assessed.Results: Plasma-concentrations of anandamide (AEA), oleoylethanolamide (OEA), palmitoyethanolamide (PEA), and pregnenolone (PREG) significantly decreased during the day. This decrease was inversely related to body mass index (AEA) or body fat (%) (PEA; OEA). The lowest RQ value, before lunch, was inversely associated with concentrations of AEA and PEA before lunch. Area under the curve (AUC) of concentrations of AEA, 2-AG, PEA, and OEA were positively related to body fat% (P <.05).The HP and MP groups showed no differences in concentrations of AEA, OEA, PEA, and PREG, but the AUC of 2-arachidonoylglycerol (2-AG) was significantly higher in the HP vs the MP group.Conclusions: In energy balance, only the endocannabinoid 2-AG changed in relation to protein level of the diet, whereas the endocannabinoid AEA and endocannabinoid-related compounds OEA and PEA reflected the gradual energy intake matching energy expenditure during the day.
AB - Context: Endocannabinoids are suggested to play a role in energy balance regulation.Objective: We aimed to investigate associations of endocannabinoid concentrations during the day with energy balance and adiposity and interactions with 2 diets differing in protein content in participants in the postobese phase with prediabetes.Design and Participants: Participants (n = 38) were individually fed in energy balance with a medium protein (MP: 15:55:30% of energy from protein:carbohydrate:fat) or high-protein diet (HP: 25:45:30% energy from P:C:F) for 48 hours in a respiration chamber.Main Outcome Measures: Associations between energy balance, energy expenditure, respiratory quotient, and endocannabinoid concentrations during the day were assessed.Results: Plasma-concentrations of anandamide (AEA), oleoylethanolamide (OEA), palmitoyethanolamide (PEA), and pregnenolone (PREG) significantly decreased during the day. This decrease was inversely related to body mass index (AEA) or body fat (%) (PEA; OEA). The lowest RQ value, before lunch, was inversely associated with concentrations of AEA and PEA before lunch. Area under the curve (AUC) of concentrations of AEA, 2-AG, PEA, and OEA were positively related to body fat% (P <.05).The HP and MP groups showed no differences in concentrations of AEA, OEA, PEA, and PREG, but the AUC of 2-arachidonoylglycerol (2-AG) was significantly higher in the HP vs the MP group.Conclusions: In energy balance, only the endocannabinoid 2-AG changed in relation to protein level of the diet, whereas the endocannabinoid AEA and endocannabinoid-related compounds OEA and PEA reflected the gradual energy intake matching energy expenditure during the day.
KW - endocannabinoids
KW - energy balance
KW - adiposity
KW - protein
KW - CIRCULATING ENDOCANNABINOIDS
KW - PERIPHERAL ENDOCANNABINOIDS
KW - BODY-WEIGHT
KW - SYSTEM
KW - OBESE
KW - DYSREGULATION
KW - EXPENDITURE
KW - METABOLISM
KW - INSULIN
KW - ADIPOSE
U2 - 10.1210/clinem/dgaa193
DO - 10.1210/clinem/dgaa193
M3 - Article
C2 - 32333763
SN - 0021-972X
VL - 105
SP - E2511-E2520
JO - Journal of Clinical Endocrinology & Metabolism
JF - Journal of Clinical Endocrinology & Metabolism
IS - 7
ER -