Abstract
Context: Endocannabinoids are suggested to play a role in energy balance regulation.
Objective: We aimed to investigate associations of endocannabinoid concentrations during the day with energy balance and adiposity and interactions with 2 diets differing in protein content in participants in the postobese phase with prediabetes.
Design and Participants: Participants (n = 38) were individually fed in energy balance with a medium protein (MP: 15:55:30% of energy from protein:carbohydrate:fat) or high-protein diet (HP: 25:45:30% energy from P:C:F) for 48 hours in a respiration chamber.
Main Outcome Measures: Associations between energy balance, energy expenditure, respiratory quotient, and endocannabinoid concentrations during the day were assessed.
Results: Plasma-concentrations of anandamide (AEA), oleoylethanolamide (OEA), palmitoyethanolamide (PEA), and pregnenolone (PREG) significantly decreased during the day. This decrease was inversely related to body mass index (AEA) or body fat (%) (PEA; OEA). The lowest RQ value, before lunch, was inversely associated with concentrations of AEA and PEA before lunch. Area under the curve (AUC) of concentrations of AEA, 2-AG, PEA, and OEA were positively related to body fat% (P <.05).
The HP and MP groups showed no differences in concentrations of AEA, OEA, PEA, and PREG, but the AUC of 2-arachidonoylglycerol (2-AG) was significantly higher in the HP vs the MP group.
Conclusions: In energy balance, only the endocannabinoid 2-AG changed in relation to protein level of the diet, whereas the endocannabinoid AEA and endocannabinoid-related compounds OEA and PEA reflected the gradual energy intake matching energy expenditure during the day.
Original language | English |
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Pages (from-to) | E2511-E2520 |
Number of pages | 10 |
Journal | Journal of Clinical Endocrinology & Metabolism |
Volume | 105 |
Issue number | 7 |
DOIs | |
Publication status | Published - Jul 2020 |
Keywords
- endocannabinoids
- energy balance
- adiposity
- protein
- CIRCULATING ENDOCANNABINOIDS
- PERIPHERAL ENDOCANNABINOIDS
- BODY-WEIGHT
- SYSTEM
- OBESE
- DYSREGULATION
- EXPENDITURE
- METABOLISM
- INSULIN
- ADIPOSE