Abstract
Original language | English |
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Article number | 7375 |
Number of pages | 12 |
Journal | Scientific Reports |
Volume | 12 |
Issue number | 1 |
DOIs | |
Publication status | Published - 5 May 2022 |
Access to Document
- 10.1038/s41598-022-10801-zLicence: CC BY
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In: Scientific Reports, Vol. 12, No. 1, 7375, 05.05.2022.
Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Reversal of diabetic-induced myopathy by swimming exercise in pregnant rats
T2 - a translational intervention study
AU - Catinelli, Bruna B.
AU - Rossignoli, Patrícia S.
AU - Floriano, Juliana F.
AU - Carr, Aline M.
AU - de Oliveira, Rafael G.
AU - dos Santos, Nilton J.
AU - Úbeda, Lara C.C.
AU - Spadella, Maria Angélica
AU - Hallur, Raghavendra L.S.
AU - Sobrevia, Luis
AU - Felisbino, Sérgio L.
AU - Calderon, Iracema M.P.
AU - Barbosa, Angélica M.P.
AU - Rudge, Marilza V.C.
AU - Souza, F. P.
AU - Berghmans, B.
AU - Thabane, L.
AU - Junginger, B.
AU - Graeff, C. F.O.
AU - Magalhães, C. G.
AU - Costa, R. A.
AU - Lima, S. A.M.
AU - Kron-Rodrigues, M. R.
AU - Barbosa, W.
AU - Campos, F. J.
AU - Bossolan, G.
AU - Corrente, J. E.
AU - Nunes, H. R.C.
AU - Abbade, J.
AU - Pedroni, C. R.
AU - Atallah, A. N.
AU - Di Bella, Z. I.K.J.
AU - Uchoa, S. M.M.
AU - Duarte, M. A.H.
AU - Mareco, E. A.
AU - Sakalem, M. E.
AU - Martinho, N.
AU - Hallur, L. S.R.
AU - Reyes, D. R.A.
AU - Alves, F. C.B.
AU - Marcondes, J. P.C.
AU - Prudencio, C. B.
AU - Pinheiro, F. A.
AU - SartorãoFilho, C. I.
AU - The Diamater Study Group
N1 - Funding Information: The authors thank the support from researchers and for the access to the infrastructure of UNIPEX (Experimental Research Unit) Botucatu Medical School—UNESP and Physiological Sciences Laboratory—School of Phylosophy and Sciences—UNESP.Special thanks to Augusto Nascimento from the Physiological Science Laboratory (Campus of Marília) and PauloGeorgete from UNIPEX (Campus of Botucatu) and Cirilo (University of Marília). Transmission electron microscopy images were obtained at the Centre of Microscopy and Image (CMI), Piracicaba Dental School, UNICAMP. Technical support of Flavia Sammartino Mariano Rodrigues is appreciated. This study was supported by FAPESP—Fundação de Amparo à Pesquisa do Estado de São Paulo (Grant number 2016/01743-5), CNPq—Conselho Nacional de DesenvolvimentoCientífico e Tecnológico to MVC Rudge (305116/2018-5). BB Catinelli received a master scholarship from FAPESP—Fundação de Amparo à Pesquisa do Estado de São Paulo (2018/03361-8), JF Floriano and RLS Hallur received Posdoc scholarships from FAPESP (Grant numbers 2017/21783-4 and 2018/02979-8). L Sobrevia thanks the support from the Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT) (Grant number 1190316), Chile, and International Sabbaticals (University Medical Centre Groningen, University of Groningen, The Netherlands) from the Vicerectorate of Academic Affairs, Academic Development Office of the Pontificia Universidad Católica de Chile. Funding Information: The authors thank the support from researchers and for the access to the infrastructure of UNIPEX (Experimental Research Unit) Botucatu Medical School—UNESP and Physiological Sciences Laboratory—School of Phylosophy and Sciences—UNESP.Special thanks to Augusto Nascimento from the Physiological Science Laboratory (Campus of Marília) and PauloGeorgete from UNIPEX (Campus of Botucatu) and Cirilo (University of Marília). Transmission electron microscopy images were obtained at the Centre of Microscopy and Image (CMI), Piracicaba Dental School, UNICAMP. Technical support of Flavia Sammartino Mariano Rodrigues is appreciated. This study was supported by FAPESP—Fundação de Amparo à Pesquisa do Estado de São Paulo (Grant number 2016/01743-5), CNPq—Conselho Nacional de DesenvolvimentoCientífico e Tecnológico to MVC Rudge (305116/2018-5). BB Catinelli received a master scholarship from FAPESP—Fundação de Amparo à Pesquisa do Estado de São Paulo (2018/03361-8), JF Floriano and RLS Hallur received Posdoc scholarships from FAPESP (Grant numbers 2017/21783-4 and 2018/02979-8). L Sobrevia thanks the support from the Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT) (Grant number 1190316), Chile, and International Sabbaticals (University Medical Centre Groningen, University of Groningen, The Netherlands) from the Vicerectorate of Academic Affairs, Academic Development Office of the Pontificia Universidad Católica de Chile. Publisher Copyright: © 2022, The Author(s).
PY - 2022/5/5
Y1 - 2022/5/5
N2 - Gestational diabetes mellitus (GDM) plus rectus abdominis muscle (RAM) myopathy predicts long-term urinary incontinence (UI). Atrophic and stiff RAM are characteristics of diabetes-induced myopathy (DiM) in pregnant rats. This study aimed to determine whether swimming exercise (SE) has a therapeutic effect in mild hyperglycemic pregnant rats model. We hypothesized that SE training might help to reverse RAM DiM. Mild hyperglycemic pregnant rats model was obtained by a unique subcutaneous injection of 100 mg/kg streptozotocin (diabetic group) or citrate buffer (non-diabetic group) on the first day of life in Wistar female newborns. At 90 days of life, the rats are mated and randomly allocated to remain sedentary or subjected to a SE protocol. The SE protocol started at gestational day 0 and consisted of 60 min/day for 6 days/week in a period of 20 days in a swim tunnel. On day 21, rats were sacrificed, and RAM was collected and studied by picrosirius red, immunohistochemistry, and transmission electron microscopy. The SE protocol increased the fiber area and diameter, and the slow-twitch and fast-twitch fiber area and diameter in the diabetic exercised group, a finding was also seen in control sedentary animals. There was a decreased type I collagen but not type III collagen area and showed a similar type I/type III ratio compared with the control sedentary group. In conclusion, SE during pregnancy reversed the RAM DiM in pregnant rats. These findings may be a potential protocol to consider in patients with RAM damage caused by GDM.
AB - Gestational diabetes mellitus (GDM) plus rectus abdominis muscle (RAM) myopathy predicts long-term urinary incontinence (UI). Atrophic and stiff RAM are characteristics of diabetes-induced myopathy (DiM) in pregnant rats. This study aimed to determine whether swimming exercise (SE) has a therapeutic effect in mild hyperglycemic pregnant rats model. We hypothesized that SE training might help to reverse RAM DiM. Mild hyperglycemic pregnant rats model was obtained by a unique subcutaneous injection of 100 mg/kg streptozotocin (diabetic group) or citrate buffer (non-diabetic group) on the first day of life in Wistar female newborns. At 90 days of life, the rats are mated and randomly allocated to remain sedentary or subjected to a SE protocol. The SE protocol started at gestational day 0 and consisted of 60 min/day for 6 days/week in a period of 20 days in a swim tunnel. On day 21, rats were sacrificed, and RAM was collected and studied by picrosirius red, immunohistochemistry, and transmission electron microscopy. The SE protocol increased the fiber area and diameter, and the slow-twitch and fast-twitch fiber area and diameter in the diabetic exercised group, a finding was also seen in control sedentary animals. There was a decreased type I collagen but not type III collagen area and showed a similar type I/type III ratio compared with the control sedentary group. In conclusion, SE during pregnancy reversed the RAM DiM in pregnant rats. These findings may be a potential protocol to consider in patients with RAM damage caused by GDM.
U2 - 10.1038/s41598-022-10801-z
DO - 10.1038/s41598-022-10801-z
M3 - Article
C2 - 35513450
SN - 2045-2322
VL - 12
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 7375
ER -