Repeatability, reproducibility, and agreement of three tonometers for measuring intraocular pressure in rabbits

Christian J F Bertens; Ralph J S van Mechelen; Tos T J M Berendschot; Marlies Gijs; Jarno E J Wolters; Theo G M F Gorgels; Rudy M M A Nuijts; Henny J M Beckers

doi:10.1038/s41598-021-98762-7

Repeatability, reproducibility, and agreement of three tonometers for measuring intraocular pressure in rabbits

Christian J F Bertens^*, Ralph J S van Mechelen, Tos T J M Berendschot, Marlies Gijs, Jarno E J Wolters, Theo G M F Gorgels, Rudy M M A Nuijts, Henny J M Beckers

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The aim of this study was to evaluate repeatability, reproducibility, and agreement of three commonly used tonometers in animal research (TonoLab, TonoVet, and TonoPEN AVIA) in a cohort of 24 rabbits. Additionally, the impact of sedation on IOP was investigated in 21 New Zealand White rabbits with the TonoVet tonometer. Repeatability was determined using the coefficient of variation (CoV) for two observers. For the TonoLab (6.55%) and TonoVet (6.38%) the CoV was lower than for the TonoPEN AVIA (10.88%). The reproducibility was highest for the TonoVet (0.2 ± 3.3 mmHg), followed by the TonoLab (0 ± 12.89 mmHg) and lowest for the TonoPEN AVIA (- 1.48 ± 10.3 mmHg). The TonoLab and TonoVet showed the highest agreement (r = 0.85, R2 = 0.73). After sedation, a significant IOP reduction (often > 25%) was observed. Our results show that among the three tonometers tested, the TonoVet tonometer is best for use in rabbits while the TonoLab should be avoided. The impact of sedation on IOP was substantial and should be taken into account during experimentation.

Original language	English
Article number	19217
Number of pages	12
Journal	Scientific Reports
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41598-021-98762-7
Publication status	Published - 28 Sept 2021

Keywords

APPLANATION TONOMETER
CENTRAL CORNEAL THICKNESS
CIRCADIAN-RHYTHM
MACAQUES MACACA-MULATTA
NONINVASIVE MEASUREMENT
OCULAR DISEASE
OPHTHALMIC DIAGNOSTIC-TESTS
REFERENCE VALUES
TEAR PRODUCTION
TONOVET(R) REBOUND TONOMETER

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Cite this

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title = "Repeatability, reproducibility, and agreement of three tonometers for measuring intraocular pressure in rabbits",

abstract = "The aim of this study was to evaluate repeatability, reproducibility, and agreement of three commonly used tonometers in animal research (TonoLab, TonoVet, and TonoPEN AVIA) in a cohort of 24 rabbits. Additionally, the impact of sedation on IOP was investigated in 21 New Zealand White rabbits with the TonoVet tonometer. Repeatability was determined using the coefficient of variation (CoV) for two observers. For the TonoLab (6.55%) and TonoVet (6.38%) the CoV was lower than for the TonoPEN AVIA (10.88%). The reproducibility was highest for the TonoVet (0.2 ± 3.3 mmHg), followed by the TonoLab (0 ± 12.89 mmHg) and lowest for the TonoPEN AVIA (- 1.48 ± 10.3 mmHg). The TonoLab and TonoVet showed the highest agreement (r = 0.85, R2 = 0.73). After sedation, a significant IOP reduction (often > 25%) was observed. Our results show that among the three tonometers tested, the TonoVet tonometer is best for use in rabbits while the TonoLab should be avoided. The impact of sedation on IOP was substantial and should be taken into account during experimentation.",

keywords = "APPLANATION TONOMETER, CENTRAL CORNEAL THICKNESS, CIRCADIAN-RHYTHM, MACAQUES MACACA-MULATTA, NONINVASIVE MEASUREMENT, OCULAR DISEASE, OPHTHALMIC DIAGNOSTIC-TESTS, REFERENCE VALUES, TEAR PRODUCTION, TONOVET(R) REBOUND TONOMETER",

author = "Bertens, {Christian J F} and {van Mechelen}, {Ralph J S} and Berendschot, {Tos T J M} and Marlies Gijs and Wolters, {Jarno E J} and Gorgels, {Theo G M F} and Nuijts, {Rudy M M A} and Beckers, {Henny J M}",

note = "{\textcopyright} 2021. The Author(s).",

year = "2021",

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day = "28",

doi = "10.1038/s41598-021-98762-7",

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T1 - Repeatability, reproducibility, and agreement of three tonometers for measuring intraocular pressure in rabbits

AU - Bertens, Christian J F

AU - van Mechelen, Ralph J S

AU - Berendschot, Tos T J M

AU - Gijs, Marlies

AU - Wolters, Jarno E J

AU - Gorgels, Theo G M F

AU - Nuijts, Rudy M M A

AU - Beckers, Henny J M

PY - 2021/9/28

Y1 - 2021/9/28

N2 - The aim of this study was to evaluate repeatability, reproducibility, and agreement of three commonly used tonometers in animal research (TonoLab, TonoVet, and TonoPEN AVIA) in a cohort of 24 rabbits. Additionally, the impact of sedation on IOP was investigated in 21 New Zealand White rabbits with the TonoVet tonometer. Repeatability was determined using the coefficient of variation (CoV) for two observers. For the TonoLab (6.55%) and TonoVet (6.38%) the CoV was lower than for the TonoPEN AVIA (10.88%). The reproducibility was highest for the TonoVet (0.2 ± 3.3 mmHg), followed by the TonoLab (0 ± 12.89 mmHg) and lowest for the TonoPEN AVIA (- 1.48 ± 10.3 mmHg). The TonoLab and TonoVet showed the highest agreement (r = 0.85, R2 = 0.73). After sedation, a significant IOP reduction (often > 25%) was observed. Our results show that among the three tonometers tested, the TonoVet tonometer is best for use in rabbits while the TonoLab should be avoided. The impact of sedation on IOP was substantial and should be taken into account during experimentation.

AB - The aim of this study was to evaluate repeatability, reproducibility, and agreement of three commonly used tonometers in animal research (TonoLab, TonoVet, and TonoPEN AVIA) in a cohort of 24 rabbits. Additionally, the impact of sedation on IOP was investigated in 21 New Zealand White rabbits with the TonoVet tonometer. Repeatability was determined using the coefficient of variation (CoV) for two observers. For the TonoLab (6.55%) and TonoVet (6.38%) the CoV was lower than for the TonoPEN AVIA (10.88%). The reproducibility was highest for the TonoVet (0.2 ± 3.3 mmHg), followed by the TonoLab (0 ± 12.89 mmHg) and lowest for the TonoPEN AVIA (- 1.48 ± 10.3 mmHg). The TonoLab and TonoVet showed the highest agreement (r = 0.85, R2 = 0.73). After sedation, a significant IOP reduction (often > 25%) was observed. Our results show that among the three tonometers tested, the TonoVet tonometer is best for use in rabbits while the TonoLab should be avoided. The impact of sedation on IOP was substantial and should be taken into account during experimentation.

KW - APPLANATION TONOMETER

KW - CENTRAL CORNEAL THICKNESS

KW - CIRCADIAN-RHYTHM

KW - MACAQUES MACACA-MULATTA

KW - NONINVASIVE MEASUREMENT

KW - OCULAR DISEASE

KW - OPHTHALMIC DIAGNOSTIC-TESTS

KW - REFERENCE VALUES

KW - TEAR PRODUCTION

KW - TONOVET(R) REBOUND TONOMETER

U2 - 10.1038/s41598-021-98762-7

DO - 10.1038/s41598-021-98762-7

M3 - Article

C2 - 34584185

SN - 2045-2322

VL - 11

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 19217

ER -