Repeatability, reproducibility, and agreement of three tonometers for measuring intraocular pressure in rabbits

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Abstract

The aim of this study was to evaluate repeatability, reproducibility, and agreement of three commonly used tonometers in animal research (TonoLab, TonoVet, and TonoPEN AVIA) in a cohort of 24 rabbits. Additionally, the impact of sedation on IOP was investigated in 21 New Zealand White rabbits with the TonoVet tonometer. Repeatability was determined using the coefficient of variation (CoV) for two observers. For the TonoLab (6.55%) and TonoVet (6.38%) the CoV was lower than for the TonoPEN AVIA (10.88%). The reproducibility was highest for the TonoVet (0.2 ± 3.3 mmHg), followed by the TonoLab (0 ± 12.89 mmHg) and lowest for the TonoPEN AVIA (- 1.48 ± 10.3 mmHg). The TonoLab and TonoVet showed the highest agreement (r = 0.85, R2 = 0.73). After sedation, a significant IOP reduction (often > 25%) was observed. Our results show that among the three tonometers tested, the TonoVet tonometer is best for use in rabbits while the TonoLab should be avoided. The impact of sedation on IOP was substantial and should be taken into account during experimentation.

Original languageEnglish
Article number19217
Pages (from-to)19217
Number of pages12
JournalScientific Reports
Volume11
Issue number1
DOIs
Publication statusPublished - 28 Sep 2021

Keywords

  • APPLANATION TONOMETER
  • CENTRAL CORNEAL THICKNESS
  • CIRCADIAN-RHYTHM
  • MACAQUES MACACA-MULATTA
  • NONINVASIVE MEASUREMENT
  • OCULAR DISEASE
  • OPHTHALMIC DIAGNOSTIC-TESTS
  • REFERENCE VALUES
  • TEAR PRODUCTION
  • TONOVET(R) REBOUND TONOMETER

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