Remodeling lipid metabolism and improving insulin responsiveness in human primary myotubes.

L.M. Sparks, C. Moro, B. Ukropcova, S. Bajpeyi, A.E. Civitarese, M.W. Hulver, G.H. Thoresen, A.C. Rustan, S.R. Smith

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Objective: Disturbances in lipid metabolism are strongly associated with insulin resistance and type 2 diabetes (T2D). We hypothesized that activation of cAMP/PKA and calcium signaling pathways in cultured human myotubes would provide further insight into regulation of lipid storage, lipolysis, lipid oxidation and insulin responsiveness.

Methods: Human myoblasts were isolated from vastus lateralis, purified, cultured and differentiated into myotubes. All cells were incubated with palmitate during differentiation. Treatment cells were pulsed 1 hour each day with forskolin and ionomycin (PFI) during the final 3 days of differentiation to activate the cAMP/PKA and calcium signaling pathways. Control cells were not pulsed (control). Mitochondrial content, (14)C lipid oxidation and storage were measured, as well as lipolysis and insulin-stimulated glycogen storage. Myotubes were stained for lipids and gene expression measured.

Results: PFI increased oxidation of oleate and palmitate to CO(2) (p <0.001), isoproterenol-stimulated lipolysis (p = 0.01), triacylglycerol (TAG) storage (p <0.05) and mitochondrial DNA copy number (p = 0.01) and related enzyme activities. Candidate gene and microarray analysis revealed increased expression of genes involved in lipolysis, TAG synthesis and mitochondrial biogenesis. PFI increased the organization of lipid droplets along the myofibrillar apparatus. These changes in lipid metabolism were associated with an increase in insulin-mediated glycogen storage (p <0.001).

Conclusions: Activation of cAMP/PKA and calcium signaling pathways in myotubes induces a remodeling of lipid droplets and functional changes in lipid metabolism. These results provide a novel pharmacological approach to promote lipid metabolism and improve insulin responsiveness in myotubes, which may be of therapeutic importance for obesity and type 2 diabetes.

Original languageEnglish
Article number21068
Number of pages10
JournalPLOS ONE
Volume6
Issue number7
DOIs
Publication statusPublished - 8 Jul 2011

Keywords

  • ADIPOSE TRIGLYCERIDE LIPASE
  • ACTIVATED PROTEIN-KINASE
  • HORMONE-SENSITIVE LIPASE
  • HUMAN SKELETAL-MUSCLE
  • INDUCED MITOCHONDRIAL BIOGENESIS
  • MAGNETIC-RESONANCE-SPECTROSCOPY
  • FATTY-ACID OXIDATION
  • ENDURANCE EXERCISE
  • GLYCOGEN-SYNTHESIS
  • GENE-EXPRESSION

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