Relationship of C5L2 receptor to skeletal muscle substrate utilization

C. Roy; S. Paglialunga; G. Schaart; E. Moonen-Kornips; R.C. Meex; E. Phielix; J. Hoeks; M.K. Hesselink; K. Cianflone; P. Schrauwen

doi:10.1371/journal.pone.0057494

Relationship of C5L2 receptor to skeletal muscle substrate utilization

C. Roy, S. Paglialunga, G. Schaart, E. Moonen-Kornips, R.C. Meex, E. Phielix, J. Hoeks, M.K. Hesselink, K. Cianflone^*, P. Schrauwen

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Objective: To investigate the role of Acylation Stimulating Protein (ASP) receptor C5L2 in skeletal muscle fatty acid accumulation and metabolism as well as insulin sensitivity in both mice and human models of diet-induced insulin resistance.

Design and Methods: Male wildtype (WT) and C5L2 knockout (KO) mice were fed a low (LFD) or a high (HFD) fat diet for 10 weeks. Intramyocellular lipid (IMCL) accumulation (by oil red O staining) and beta-oxidation HADH enzyme activity were determined in skeletal muscle. Mitochondria were isolated from hindleg muscles for high-resolution respirometry. Muscle C5L2 protein content was also determined in obese type 2 diabetics and age- and BMI matched men.

Results: IMCL levels were increased by six-fold in C5L2KO-HFD compared to WT-HFD mice (p

Conclusion: The results suggest that insulin sensitivity may be permissive for coupling of C5L2 levels to lipid storage and utilization.

Original language	English
Article number	57494
Number of pages	8
Journal	PLOS ONE
Volume	8
Issue number	2
DOIs	https://doi.org/10.1371/journal.pone.0057494
Publication status	Published - 27 Feb 2013

Keywords

ACYLATION-STIMULATING PROTEIN
INDUCED INSULIN-RESISTANCE
FATTY-ACID OXIDATION
LIPOPROTEIN-LIPASE
ADIPOSE-TISSUE
TRIACYLGLYCEROL SYNTHESIS
TRIGLYCERIDE SYNTHESIS
GLUCOSE-TRANSPORT
LIPID-CONTENT
IN-VIVO

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Cite this

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title = "Relationship of C5L2 receptor to skeletal muscle substrate utilization",

abstract = "Objective: To investigate the role of Acylation Stimulating Protein (ASP) receptor C5L2 in skeletal muscle fatty acid accumulation and metabolism as well as insulin sensitivity in both mice and human models of diet-induced insulin resistance.Design and Methods: Male wildtype (WT) and C5L2 knockout (KO) mice were fed a low (LFD) or a high (HFD) fat diet for 10 weeks. Intramyocellular lipid (IMCL) accumulation (by oil red O staining) and beta-oxidation HADH enzyme activity were determined in skeletal muscle. Mitochondria were isolated from hindleg muscles for high-resolution respirometry. Muscle C5L2 protein content was also determined in obese type 2 diabetics and age- and BMI matched men.Results: IMCL levels were increased by six-fold in C5L2KO-HFD compared to WT-HFD mice (pConclusion: The results suggest that insulin sensitivity may be permissive for coupling of C5L2 levels to lipid storage and utilization.",

keywords = "ACYLATION-STIMULATING PROTEIN, INDUCED INSULIN-RESISTANCE, FATTY-ACID OXIDATION, LIPOPROTEIN-LIPASE, ADIPOSE-TISSUE, TRIACYLGLYCEROL SYNTHESIS, TRIGLYCERIDE SYNTHESIS, GLUCOSE-TRANSPORT, LIPID-CONTENT, IN-VIVO",

author = "C. Roy and S. Paglialunga and G. Schaart and E. Moonen-Kornips and R.C. Meex and E. Phielix and J. Hoeks and M.K. Hesselink and K. Cianflone and P. Schrauwen",

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doi = "10.1371/journal.pone.0057494",

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T1 - Relationship of C5L2 receptor to skeletal muscle substrate utilization

AU - Roy, C.

AU - Paglialunga, S.

AU - Schaart, G.

AU - Moonen-Kornips, E.

AU - Meex, R.C.

AU - Phielix, E.

AU - Hoeks, J.

AU - Hesselink, M.K.

AU - Cianflone, K.

AU - Schrauwen, P.

PY - 2013/2/27

Y1 - 2013/2/27

N2 - Objective: To investigate the role of Acylation Stimulating Protein (ASP) receptor C5L2 in skeletal muscle fatty acid accumulation and metabolism as well as insulin sensitivity in both mice and human models of diet-induced insulin resistance.Design and Methods: Male wildtype (WT) and C5L2 knockout (KO) mice were fed a low (LFD) or a high (HFD) fat diet for 10 weeks. Intramyocellular lipid (IMCL) accumulation (by oil red O staining) and beta-oxidation HADH enzyme activity were determined in skeletal muscle. Mitochondria were isolated from hindleg muscles for high-resolution respirometry. Muscle C5L2 protein content was also determined in obese type 2 diabetics and age- and BMI matched men.Results: IMCL levels were increased by six-fold in C5L2KO-HFD compared to WT-HFD mice (pConclusion: The results suggest that insulin sensitivity may be permissive for coupling of C5L2 levels to lipid storage and utilization.

AB - Objective: To investigate the role of Acylation Stimulating Protein (ASP) receptor C5L2 in skeletal muscle fatty acid accumulation and metabolism as well as insulin sensitivity in both mice and human models of diet-induced insulin resistance.Design and Methods: Male wildtype (WT) and C5L2 knockout (KO) mice were fed a low (LFD) or a high (HFD) fat diet for 10 weeks. Intramyocellular lipid (IMCL) accumulation (by oil red O staining) and beta-oxidation HADH enzyme activity were determined in skeletal muscle. Mitochondria were isolated from hindleg muscles for high-resolution respirometry. Muscle C5L2 protein content was also determined in obese type 2 diabetics and age- and BMI matched men.Results: IMCL levels were increased by six-fold in C5L2KO-HFD compared to WT-HFD mice (pConclusion: The results suggest that insulin sensitivity may be permissive for coupling of C5L2 levels to lipid storage and utilization.

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KW - TRIACYLGLYCEROL SYNTHESIS

KW - TRIGLYCERIDE SYNTHESIS

KW - GLUCOSE-TRANSPORT

KW - LIPID-CONTENT

KW - IN-VIVO

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