Objective: To investigate the role of Acylation Stimulating Protein (ASP) receptor C5L2 in skeletal muscle fatty acid accumulation and metabolism as well as insulin sensitivity in both mice and human models of diet-induced insulin resistance.
Design and Methods: Male wildtype (WT) and C5L2 knockout (KO) mice were fed a low (LFD) or a high (HFD) fat diet for 10 weeks. Intramyocellular lipid (IMCL) accumulation (by oil red O staining) and beta-oxidation HADH enzyme activity were determined in skeletal muscle. Mitochondria were isolated from hindleg muscles for high-resolution respirometry. Muscle C5L2 protein content was also determined in obese type 2 diabetics and age- and BMI matched men.
Results: IMCL levels were increased by six-fold in C5L2KO-HFD compared to WT-HFD mice (p
Conclusion: The results suggest that insulin sensitivity may be permissive for coupling of C5L2 levels to lipid storage and utilization.
- ACYLATION-STIMULATING PROTEIN
- INDUCED INSULIN-RESISTANCE
- FATTY-ACID OXIDATION
- TRIACYLGLYCEROL SYNTHESIS
- TRIGLYCERIDE SYNTHESIS