@article{6a6ee2ee0f51436cad43de18b825cce2,
title = "Quantify permeability using on-a-chip models in high-throughput applications",
abstract = "Traditionally, to quantify permeability of a biological barrier, the initial slope is used, based on the assumption of sink condition (concentration of the donor is constant, and the receiver increases less than 10%). With on-a-chip barrier models, this assumption fails in cell-free or leaky conditions, which requires the use of the exact solution. To encounter a time delay from performing the assay and acquiring the data, we present a protocol with the exact equation modified to incorporate a time offset.",
keywords = "Biotechnology and bioengineering, Cell-based Assays, High-throughput Screening, Microscopy",
author = "Camilla Soragni and Tessa Vergroesen and Nynke Hettema and Gwena{\"e}lle Rabussier and Lanz, {Henri{\"e}tte L.} and Trietsch, {Sebastian J.} and {de Windt}, {Leon J.} and Ng, {Chee P.}",
note = "Funding Information: This work was supported by the European Union's Horizon 2020 Research and Innovation Program iPlacenta under grant agreement no. 765274. Conceptualization, C.P.N. C.S.; methodology, C.P.N. C.S.; validation, C.S. T.V. N.H.; formal analysis, C.P.N. C.S. T.V. N.H.; investigation, C.S. T.V. N.H.; writing and editing, C.S. C.P.; visualization, H.L.L. C.S. G.R.; supervision, C.P.N. L.J.d.W. S.J.T. C.S. T.V. and N.H. were employed by MIMETAS BV when the data were generated; G.R. H.L.L. S.J.T. and C.P.N. are employees of Mimetas BV, which is marketing the OrganoPlate; and S.J.T. is a shareholder of Mimetas BV. OrganoPlate is a registered trademark of Mimetas BV. Funding Information: This work was supported by the European Union{\textquoteright}s Horizon 2020 Research and Innovation Program iPlacenta under grant agreement no. 765274 . Publisher Copyright: {\textcopyright} 2023 The Author(s)",
year = "2023",
month = mar,
day = "17",
doi = "10.1016/j.xpro.2023.102051",
language = "English",
volume = "4",
journal = "STAR protocols",
issn = "2666-1667",
publisher = "Elsevier",
number = "1",
}