Quantify permeability using on-a-chip models in high-throughput applications

Camilla Soragni*, Tessa Vergroesen, Nynke Hettema, Gwenaëlle Rabussier, Henriëtte L. Lanz, Sebastian J. Trietsch, Leon J. de Windt, Chee P. Ng*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Traditionally, to quantify permeability of a biological barrier, the initial slope is used, based on the assumption of sink condition (concentration of the donor is constant, and the receiver increases less than 10%). With on-a-chip barrier models, this assumption fails in cell-free or leaky conditions, which requires the use of the exact solution. To encounter a time delay from performing the assay and acquiring the data, we present a protocol with the exact equation modified to incorporate a time offset.
Original languageEnglish
Article number102051
Number of pages21
JournalSTAR protocols
Volume4
Issue number1
DOIs
Publication statusPublished - 17 Mar 2023

Keywords

  • Biotechnology and bioengineering
  • Cell-based Assays
  • High-throughput Screening
  • Microscopy

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