Potential of High- and Low-Acetylated Galactoglucomannooligosaccharides as Modulators of the Microbiota Composition and Their Activity: A Comparison Using the In Vitro Model of the Human Colon TIM-2

Beatriz Miguez, Carlos Vila, Koen Venema, Juan C. Parajo, Jose L. Alonso*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

High- and low-acetylated galactoglucomannooligosaccharides (GGMOS_Ac and GGMOS, respectively) were assayed as substrates in the TIM-2 in vitro colon model using, as inoculum, fecal microbiota from the elderly. The effects on the microbiota and their activity were also compared to a standard ileal efflux medium (SIEM). GGMOS resulted in higher organic acid productions and higher short-chain fatty acids/total organic acid molar ratios. Although comparable Actinobacteria abundances were observed with both substrates, GGMOS fermentation led to higher Firmicutes/Bacteroidetes ratios and lower Proteobacteria percentages than GGMOS_Ac. No differences were found concerning the percentages of beneficial genus such as Blautia, Faecalibacterium, Coprococcus, or Bifidobacterium. However, higher bacterial diversities and numbers of genera such as Oscillospira and Lachnospira were found with GGMOS_Ac. This suggests that GGMOS would be more suitable substrates for the elderly, even though GGMOS_Ac promoted positive effects that support the interest of further research using these oligosaccharides as "carriers" of desired substituents.

Original languageEnglish
Pages (from-to)7617-7629
Number of pages13
JournalJournal of Agricultural and Food Chemistry
Volume68
Issue number29
DOIs
Publication statusPublished - 22 Jul 2020

Keywords

  • galactoglucomannooligosaccaharides
  • GGMOS
  • Pinus pinaster
  • acetylated
  • SIEM
  • prebiotics
  • gut micro biota
  • elderly
  • TIM-2
  • CHAIN FATTY-ACIDS
  • GUT MICROBIOTA
  • FERMENTATION
  • PREBIOTICS
  • BIFIDOBACTERIA
  • HEALTH
  • IMPACT
  • WOOD
  • PROBIOTICS
  • PRODUCTS

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