Abstract
Modern drug development increasingly requires comprehensive models that can be utilized in the earliest stages of compound and target discovery. Here we report a phenotypic screening exercise in a high-throughput Organ-on-a-Chip setup. We assessed the inhibitory effect of 1537 protein kinase inhibitors in an angiogenesis assay. Over 4000 micro-vessels were grown under perfusion flow in microfluidic chips, exposed to a cocktail of pro-angiogenic factors and subsequently exposed to the respective kinase inhibitors. Efficacy of compounds was evaluated by reduced angiogenic sprouting, whereas reduced integrity of the main micro-vessel was taken as a measure for toxicity. The screen yielded 53 hits with high anti-angiogenicity and low toxicity, of which 44 were previously unassociated with angiogenic pathways. This study demonstrates that Organ-on-a-Chip models can be screened in high numbers to identify novel compounds and targets. This will ultimately reduce bias in early-stage drug development and increases probability to identify first in class compounds and targets for today's intractable diseases.
Original language | English |
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Pages (from-to) | 37-49 |
Number of pages | 13 |
Journal | Angiogenesis |
Volume | 27 |
Issue number | 1 |
Early online date | 1 Jul 2023 |
DOIs | |
Publication status | Published - Feb 2024 |
Keywords
- High throughput screening
- Angiogenesis
- Protein kinase inhibitors
- Phenotypic screening
- Organ-on-a-Chip
- Microphysiological systems
- REGORAFENIB
- SELECTION
- CHEMBL
- VEGF