TY - JOUR
T1 - Oxidative stress in healthy pregnancy and preeclampsia is linked to chronic inflammation, iron status and vascular function
AU - Mannaerts, Dominique
AU - Faes, Ellen
AU - Cos, Paul
AU - Briede, Jacob J.
AU - Gyselaers, Wilfried
AU - Cornette, Jerome
AU - Gorbanev, Yury
AU - Bogaerts, Annemie
AU - Spaanderman, Marc
AU - Van Craenenbroeck, Emeline
AU - Jacquemyn, Yves
PY - 2018/9/11
Y1 - 2018/9/11
N2 - BackgroundDuring normal pregnancy, placental oxidative stress (OS) is present during all three trimesters and is necessary to obtain normal cell function. However, if OS reaches a certain level, pregnancy complications might arise. In preeclampsia (PE), a dangerous pregnancy specific hypertensive disorder, OS induced in the ischemic placenta causes a systemic inflammatory response and activates maternal endothelial cells. In this study, we aimed to quantify superoxide concentrations (as a measure of systemic OS) using electron paramagnetic resonance (EPR) and correlate them to markers of systemic inflammation, iron status and vascular function.MethodsFifty-nine women with a healthy pregnancy (HP), 10 non-pregnant controls (NP) and 28 PE patients (32 +/- 3.3weeks) were included. During HP, blood samples for superoxide, neutrophil to lymphocyte ratio (NLR), mean platelet volume (MPV) and iron status were taken at 10, 25 and 39 weeks. Vascular measurements for arterial stiffness (carotid-femoral pulse wave velocity (CF-PWV), augmentation index (Alx), augmentation Pressure (AP)) and microvascular endothelial function (reactive hyperemia index (RHI)) were performed at 35 weeks. In PE, all measurements were performed at diagnosis. CMH (1-hydroxy-3-methoxycarbonyl- 2,2,5,5-tetramethylpyrrolidine) was used as spin probe for EPR, since the formed CM radical corresponds to the amount of superoxide.ResultsSuperoxide concentration remains stable during pregnancy (p = 0.92), but is significantly higher compared to the NP controls (pConclusionsDuring HP there is an increased but stable oxidative environment, which is correlated to ferritin concentration. If superoxide levels increase, there is an augmentation in arterial stiffness. In PE pregnancies, systemic inflammation and superoxide concentrations are higher and result in a deterioration of endothelial function. Together, these findings support the hypothesis that vascular function is directly linked to the amount of OS and that measurement of OS in combination with vascular function tests might be used in the prediction of PE.
AB - BackgroundDuring normal pregnancy, placental oxidative stress (OS) is present during all three trimesters and is necessary to obtain normal cell function. However, if OS reaches a certain level, pregnancy complications might arise. In preeclampsia (PE), a dangerous pregnancy specific hypertensive disorder, OS induced in the ischemic placenta causes a systemic inflammatory response and activates maternal endothelial cells. In this study, we aimed to quantify superoxide concentrations (as a measure of systemic OS) using electron paramagnetic resonance (EPR) and correlate them to markers of systemic inflammation, iron status and vascular function.MethodsFifty-nine women with a healthy pregnancy (HP), 10 non-pregnant controls (NP) and 28 PE patients (32 +/- 3.3weeks) were included. During HP, blood samples for superoxide, neutrophil to lymphocyte ratio (NLR), mean platelet volume (MPV) and iron status were taken at 10, 25 and 39 weeks. Vascular measurements for arterial stiffness (carotid-femoral pulse wave velocity (CF-PWV), augmentation index (Alx), augmentation Pressure (AP)) and microvascular endothelial function (reactive hyperemia index (RHI)) were performed at 35 weeks. In PE, all measurements were performed at diagnosis. CMH (1-hydroxy-3-methoxycarbonyl- 2,2,5,5-tetramethylpyrrolidine) was used as spin probe for EPR, since the formed CM radical corresponds to the amount of superoxide.ResultsSuperoxide concentration remains stable during pregnancy (p = 0.92), but is significantly higher compared to the NP controls (pConclusionsDuring HP there is an increased but stable oxidative environment, which is correlated to ferritin concentration. If superoxide levels increase, there is an augmentation in arterial stiffness. In PE pregnancies, systemic inflammation and superoxide concentrations are higher and result in a deterioration of endothelial function. Together, these findings support the hypothesis that vascular function is directly linked to the amount of OS and that measurement of OS in combination with vascular function tests might be used in the prediction of PE.
KW - PARAMETERS
KW - BLOOD
KW - SUPEROXIDE
KW - TONOMETRY
KW - ISCHEMIA
KW - DISEASE
KW - DAMAGE
KW - COUNT
KW - RATIO
KW - DNA
U2 - 10.1371/journal.pone.0202919
DO - 10.1371/journal.pone.0202919
M3 - Article
C2 - 30204759
SN - 1932-6203
VL - 13
JO - PLOS ONE
JF - PLOS ONE
IS - 9
M1 - 0202919
ER -