TY - JOUR
T1 - New Large Animal Model for Aortic Aneurysms in the Viscerorenal Segment
AU - Kalder, Johannes
AU - Isfort, Peter
AU - Reinartz, Sebastian Daniel
AU - Gremse, Felix
AU - Yamoah, Grace Gyamfuah
AU - Gesche, Valentine
AU - Kotelis, Drosos
AU - Tolba, Rene
AU - Jacobs, Michael Johan
AU - Jalaie, Houman
N1 - Funding Information:
The project was funded by the “Ziel2”-program of Nordrhein-Westfalen , Germany. G.G.Y. acknowledges a stipend from the German Academic Exchange Service (DAAD). The authors thank Z. Packsereshtmogharab for organizational assistance during the entire project and Dr M Afify for evaluating the histology. Furthermore, the authors also thank the team of the Animal Research Institute, especially T. Stopinski, for the good performance with anesthesia and for support during the experiments in the animal operation theater. The authors also thank K. Scherer for the high-end support during the project planning. Soli deo Gloria.
Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/8
Y1 - 2019/8
N2 - Background: Aortic aneurysms in the viscerorenal-segment are nowadays treatable by endovascular means. Previously, new endograft techniques were only tested in healthy animals. We aimed to establish a new large animal model for testing complex endovascular stent techniques preclinically.Methods: In sheep, four juxtarenal and two type IV thoracoabdominal aortic aneurysms were surgically created via a retroperitoneal approach. Two pieces out of a 10 x 15-cm bovine pericardial patch were sewn with the healthy aorta longitudinally. The viscerorenal segment was clamped, and the aorta was incised longitudinally. Then, the patches were longitudinally sewn together. In the meantime, antegrade flow through the native part of the aorta was already established by tangential clamping. Computed tomography angiography was performed after 4, 8, and 52 wk.Results: Technical success was 100%. The median surgical procedure time was 3 h, the median blood loss was 210 mL, and the viscerorenal-segment clamping time was 2-4 min. The animals started drinking 1 h after arousal from anesthesia. One animal died after 1 wk because of delayed bleeding and another died after 1 y because of aneurysm rupture by a secondary bacterial infection. Four animals survived. The proximal landing zone diameter and the clock position of the vessel were stable over 52 wk.Conclusions: Surgical creation of an aortic aneurysm in the viscerorenal-segment in sheep was successful, without an ischemia/reperfusion injury. This animal model offers a new platform for evaluating innovative endovascular therapy options in vivo. (C) 2019 Elsevier Inc. All rights reserved.
AB - Background: Aortic aneurysms in the viscerorenal-segment are nowadays treatable by endovascular means. Previously, new endograft techniques were only tested in healthy animals. We aimed to establish a new large animal model for testing complex endovascular stent techniques preclinically.Methods: In sheep, four juxtarenal and two type IV thoracoabdominal aortic aneurysms were surgically created via a retroperitoneal approach. Two pieces out of a 10 x 15-cm bovine pericardial patch were sewn with the healthy aorta longitudinally. The viscerorenal segment was clamped, and the aorta was incised longitudinally. Then, the patches were longitudinally sewn together. In the meantime, antegrade flow through the native part of the aorta was already established by tangential clamping. Computed tomography angiography was performed after 4, 8, and 52 wk.Results: Technical success was 100%. The median surgical procedure time was 3 h, the median blood loss was 210 mL, and the viscerorenal-segment clamping time was 2-4 min. The animals started drinking 1 h after arousal from anesthesia. One animal died after 1 wk because of delayed bleeding and another died after 1 y because of aneurysm rupture by a secondary bacterial infection. Four animals survived. The proximal landing zone diameter and the clock position of the vessel were stable over 52 wk.Conclusions: Surgical creation of an aortic aneurysm in the viscerorenal-segment in sheep was successful, without an ischemia/reperfusion injury. This animal model offers a new platform for evaluating innovative endovascular therapy options in vivo. (C) 2019 Elsevier Inc. All rights reserved.
KW - Aorta
KW - Aortic aneurysm
KW - Models
KW - Animal
KW - Endovascular procedures
KW - STENT-GRAFTS
KW - MANAGEMENT
KW - REPAIR
U2 - 10.1016/j.jss.2019.02.054
DO - 10.1016/j.jss.2019.02.054
M3 - Article
C2 - 30933829
SN - 0022-4804
VL - 240
SP - 156
EP - 164
JO - Journal of Surgical Research
JF - Journal of Surgical Research
ER -