N-acetyl resonances in in vivo and in vitro NMR spectroscopy of cystic ovarian tumors.

E. Kolwijck, U.F. Engelke, M. van der Graaf, A. Heerschap, H.J. Blom, M. Hadfoune, W.A. Buurman, L.F. Massuger, R.A. Wevers

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    Abstract

    An unassigned and prominent resonance in the region from delta 2.0-2.1 ppm has frequently been found in the in vivo MR spectra of cancer patients. We demonstrated the presence of this resonance with in vivo MRS in the cyst fluid of a patient with an ovarian tumor. (1)H-NMRS on the aspirated cyst fluid of this patient confirmed the observation. A complex of resonances was observed between 2.0 and 2.1 ppm. It was also present in 11 additional ovarian cyst fluid samples randomly chosen from our biobank. The resonance complex was significantly more prominent in samples from mucinous tumors than in samples from other histological subtypes. A macromolecule (>10 kDa) was found responsible for this complex of resonances. A correlation spectroscopy (COSY) experiment revealed cross peaks of two different types of bound sialic acid suggesting that N-glycans from glycoproteins and/or glycolipids cause this resonance complex. In the literature, plasma alpha-1 acid glycoprotein (AGP), known for its high content of N-linked glycans, has been suggested to contribute to the delta 2.0-2.1 spectral region. The AGP cyst fluid concentration did not correlate significantly with the peak height of the delta 2.0-2.1 resonance complex in our study. AGP may be partly responsible for the resonance complex but other N-acetylated glycoproteins and/or glycolipids also contribute. After deproteinization of the cyst fluid, N-acetyl-L-aspartic acid (NAA) was found to contribute significantly to the signal in this spectral region in three of the 12 samples. GC-MS independently confirmed the presence of NAA in high concentration in the three samples, which all derived from benign serous tumors. We conclude that both NAA and N-acetyl groups from glycoproteins and/or glycolipids may contribute to the delta 2.0-2.1 ppm resonance complex in ovarian cyst fluid. This spectral region seems to contain resonances from biomarkers that provide relevant clinical information on the type of ovarian tumor. Copyright (c) 2009 John Wiley & Sons, Ltd.
    Original languageEnglish
    Pages (from-to)1093-1099
    JournalNmr in Biomedicine
    Volume22
    Issue number10
    DOIs
    Publication statusPublished - 1 Jan 2009

    Cite this

    Kolwijck, E., Engelke, U. F., van der Graaf, M., Heerschap, A., Blom, H. J., Hadfoune, M., ... Wevers, R. A. (2009). N-acetyl resonances in in vivo and in vitro NMR spectroscopy of cystic ovarian tumors. Nmr in Biomedicine, 22(10), 1093-1099. https://doi.org/10.1002/nbm.1417
    Kolwijck, E. ; Engelke, U.F. ; van der Graaf, M. ; Heerschap, A. ; Blom, H.J. ; Hadfoune, M. ; Buurman, W.A. ; Massuger, L.F. ; Wevers, R.A. / N-acetyl resonances in in vivo and in vitro NMR spectroscopy of cystic ovarian tumors. In: Nmr in Biomedicine. 2009 ; Vol. 22, No. 10. pp. 1093-1099.
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    title = "N-acetyl resonances in in vivo and in vitro NMR spectroscopy of cystic ovarian tumors.",
    abstract = "An unassigned and prominent resonance in the region from delta 2.0-2.1 ppm has frequently been found in the in vivo MR spectra of cancer patients. We demonstrated the presence of this resonance with in vivo MRS in the cyst fluid of a patient with an ovarian tumor. (1)H-NMRS on the aspirated cyst fluid of this patient confirmed the observation. A complex of resonances was observed between 2.0 and 2.1 ppm. It was also present in 11 additional ovarian cyst fluid samples randomly chosen from our biobank. The resonance complex was significantly more prominent in samples from mucinous tumors than in samples from other histological subtypes. A macromolecule (>10 kDa) was found responsible for this complex of resonances. A correlation spectroscopy (COSY) experiment revealed cross peaks of two different types of bound sialic acid suggesting that N-glycans from glycoproteins and/or glycolipids cause this resonance complex. In the literature, plasma alpha-1 acid glycoprotein (AGP), known for its high content of N-linked glycans, has been suggested to contribute to the delta 2.0-2.1 spectral region. The AGP cyst fluid concentration did not correlate significantly with the peak height of the delta 2.0-2.1 resonance complex in our study. AGP may be partly responsible for the resonance complex but other N-acetylated glycoproteins and/or glycolipids also contribute. After deproteinization of the cyst fluid, N-acetyl-L-aspartic acid (NAA) was found to contribute significantly to the signal in this spectral region in three of the 12 samples. GC-MS independently confirmed the presence of NAA in high concentration in the three samples, which all derived from benign serous tumors. We conclude that both NAA and N-acetyl groups from glycoproteins and/or glycolipids may contribute to the delta 2.0-2.1 ppm resonance complex in ovarian cyst fluid. This spectral region seems to contain resonances from biomarkers that provide relevant clinical information on the type of ovarian tumor. Copyright (c) 2009 John Wiley & Sons, Ltd.",
    author = "E. Kolwijck and U.F. Engelke and {van der Graaf}, M. and A. Heerschap and H.J. Blom and M. Hadfoune and W.A. Buurman and L.F. Massuger and R.A. Wevers",
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    Kolwijck, E, Engelke, UF, van der Graaf, M, Heerschap, A, Blom, HJ, Hadfoune, M, Buurman, WA, Massuger, LF & Wevers, RA 2009, 'N-acetyl resonances in in vivo and in vitro NMR spectroscopy of cystic ovarian tumors.', Nmr in Biomedicine, vol. 22, no. 10, pp. 1093-1099. https://doi.org/10.1002/nbm.1417

    N-acetyl resonances in in vivo and in vitro NMR spectroscopy of cystic ovarian tumors. / Kolwijck, E.; Engelke, U.F.; van der Graaf, M.; Heerschap, A.; Blom, H.J.; Hadfoune, M.; Buurman, W.A.; Massuger, L.F.; Wevers, R.A.

    In: Nmr in Biomedicine, Vol. 22, No. 10, 01.01.2009, p. 1093-1099.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - N-acetyl resonances in in vivo and in vitro NMR spectroscopy of cystic ovarian tumors.

    AU - Kolwijck, E.

    AU - Engelke, U.F.

    AU - van der Graaf, M.

    AU - Heerschap, A.

    AU - Blom, H.J.

    AU - Hadfoune, M.

    AU - Buurman, W.A.

    AU - Massuger, L.F.

    AU - Wevers, R.A.

    PY - 2009/1/1

    Y1 - 2009/1/1

    N2 - An unassigned and prominent resonance in the region from delta 2.0-2.1 ppm has frequently been found in the in vivo MR spectra of cancer patients. We demonstrated the presence of this resonance with in vivo MRS in the cyst fluid of a patient with an ovarian tumor. (1)H-NMRS on the aspirated cyst fluid of this patient confirmed the observation. A complex of resonances was observed between 2.0 and 2.1 ppm. It was also present in 11 additional ovarian cyst fluid samples randomly chosen from our biobank. The resonance complex was significantly more prominent in samples from mucinous tumors than in samples from other histological subtypes. A macromolecule (>10 kDa) was found responsible for this complex of resonances. A correlation spectroscopy (COSY) experiment revealed cross peaks of two different types of bound sialic acid suggesting that N-glycans from glycoproteins and/or glycolipids cause this resonance complex. In the literature, plasma alpha-1 acid glycoprotein (AGP), known for its high content of N-linked glycans, has been suggested to contribute to the delta 2.0-2.1 spectral region. The AGP cyst fluid concentration did not correlate significantly with the peak height of the delta 2.0-2.1 resonance complex in our study. AGP may be partly responsible for the resonance complex but other N-acetylated glycoproteins and/or glycolipids also contribute. After deproteinization of the cyst fluid, N-acetyl-L-aspartic acid (NAA) was found to contribute significantly to the signal in this spectral region in three of the 12 samples. GC-MS independently confirmed the presence of NAA in high concentration in the three samples, which all derived from benign serous tumors. We conclude that both NAA and N-acetyl groups from glycoproteins and/or glycolipids may contribute to the delta 2.0-2.1 ppm resonance complex in ovarian cyst fluid. This spectral region seems to contain resonances from biomarkers that provide relevant clinical information on the type of ovarian tumor. Copyright (c) 2009 John Wiley & Sons, Ltd.

    AB - An unassigned and prominent resonance in the region from delta 2.0-2.1 ppm has frequently been found in the in vivo MR spectra of cancer patients. We demonstrated the presence of this resonance with in vivo MRS in the cyst fluid of a patient with an ovarian tumor. (1)H-NMRS on the aspirated cyst fluid of this patient confirmed the observation. A complex of resonances was observed between 2.0 and 2.1 ppm. It was also present in 11 additional ovarian cyst fluid samples randomly chosen from our biobank. The resonance complex was significantly more prominent in samples from mucinous tumors than in samples from other histological subtypes. A macromolecule (>10 kDa) was found responsible for this complex of resonances. A correlation spectroscopy (COSY) experiment revealed cross peaks of two different types of bound sialic acid suggesting that N-glycans from glycoproteins and/or glycolipids cause this resonance complex. In the literature, plasma alpha-1 acid glycoprotein (AGP), known for its high content of N-linked glycans, has been suggested to contribute to the delta 2.0-2.1 spectral region. The AGP cyst fluid concentration did not correlate significantly with the peak height of the delta 2.0-2.1 resonance complex in our study. AGP may be partly responsible for the resonance complex but other N-acetylated glycoproteins and/or glycolipids also contribute. After deproteinization of the cyst fluid, N-acetyl-L-aspartic acid (NAA) was found to contribute significantly to the signal in this spectral region in three of the 12 samples. GC-MS independently confirmed the presence of NAA in high concentration in the three samples, which all derived from benign serous tumors. We conclude that both NAA and N-acetyl groups from glycoproteins and/or glycolipids may contribute to the delta 2.0-2.1 ppm resonance complex in ovarian cyst fluid. This spectral region seems to contain resonances from biomarkers that provide relevant clinical information on the type of ovarian tumor. Copyright (c) 2009 John Wiley & Sons, Ltd.

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    Kolwijck E, Engelke UF, van der Graaf M, Heerschap A, Blom HJ, Hadfoune M et al. N-acetyl resonances in in vivo and in vitro NMR spectroscopy of cystic ovarian tumors. Nmr in Biomedicine. 2009 Jan 1;22(10):1093-1099. https://doi.org/10.1002/nbm.1417