Mineralization-defects are comparable in fluorotic impacted human teeth and fluorotic mouse incisors

Rozita Jalali, Samaneh Ghazanfari, Franck Guy, Don Lyaruu, Leo van Ruijve, Pamela DenBesten, Stefania Martignon, Gina Castiblanco, Antonius Bronckers

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Objective: Fluoride excess of 0.05-0.07 mg F/kg bw/day in water or food additives like salt is the principal cause of endemic dental fluorosis. How fluoride causes these defects is not clear yet. Recent studies in rodents suggest that development of enamel fluorosis is associated with insufficient neutralization of protons released during the formation of hypermineralized lines.

Design: Here we examined whether hypermineralization could also be assessed by MicroCT in developing molar enamel of humans exposed to fluoride.

Result Micro-CT analysis of hypomineralized enamel from human fluorotic molars graded by the Thylstrup Fejerskov(TF) Index as showed weak hypermineralized lines and hypermineralized patches not seen in TF-I/II grade enamel. The mesio-distal sides of these molar teeth were significantly smaller (similar to 18%, p = 0.02) than in TF-I/II teeth.

Conclusion: The patterns of changes observed in human fluorotic teeth were similar to those in fluorotic rodent incisors. The data are consistent with the hypothesis that also in developing human teeth fluoride-stimulated local acidification of enamel could be a mechanism for developing fluorotic enamel.

Original languageEnglish
Pages (from-to)214-221
Number of pages8
JournalArchives of Oral Biology
Volume83
DOIs
Publication statusPublished - Nov 2017

Keywords

  • AMELOBLASTS
  • AMELOGENESIS
  • DENTAL FLUOROSIS
  • Dental fluorosis
  • ENAMEL FLUOROSIS
  • EXPOSURE
  • Enamel development
  • FLUORIDE
  • Hypermineralization
  • Hypomineralization
  • MODULATION
  • ORGAN-CULTURE
  • RAT INCISORS
  • SECRETORY-STAGE

Cite this

Jalali, Rozita ; Ghazanfari, Samaneh ; Guy, Franck ; Lyaruu, Don ; van Ruijve, Leo ; DenBesten, Pamela ; Martignon, Stefania ; Castiblanco, Gina ; Bronckers, Antonius . / Mineralization-defects are comparable in fluorotic impacted human teeth and fluorotic mouse incisors. In: Archives of Oral Biology. 2017 ; Vol. 83. pp. 214-221.
@article{dea5dd8aca954186a833784590f8ee0f,
title = "Mineralization-defects are comparable in fluorotic impacted human teeth and fluorotic mouse incisors",
abstract = "Objective: Fluoride excess of 0.05-0.07 mg F/kg bw/day in water or food additives like salt is the principal cause of endemic dental fluorosis. How fluoride causes these defects is not clear yet. Recent studies in rodents suggest that development of enamel fluorosis is associated with insufficient neutralization of protons released during the formation of hypermineralized lines.Design: Here we examined whether hypermineralization could also be assessed by MicroCT in developing molar enamel of humans exposed to fluoride.Result Micro-CT analysis of hypomineralized enamel from human fluorotic molars graded by the Thylstrup Fejerskov(TF) Index as showed weak hypermineralized lines and hypermineralized patches not seen in TF-I/II grade enamel. The mesio-distal sides of these molar teeth were significantly smaller (similar to 18{\%}, p = 0.02) than in TF-I/II teeth.Conclusion: The patterns of changes observed in human fluorotic teeth were similar to those in fluorotic rodent incisors. The data are consistent with the hypothesis that also in developing human teeth fluoride-stimulated local acidification of enamel could be a mechanism for developing fluorotic enamel.",
keywords = "AMELOBLASTS, AMELOGENESIS, DENTAL FLUOROSIS, Dental fluorosis, ENAMEL FLUOROSIS, EXPOSURE, Enamel development, FLUORIDE, Hypermineralization, Hypomineralization, MODULATION, ORGAN-CULTURE, RAT INCISORS, SECRETORY-STAGE",
author = "Rozita Jalali and Samaneh Ghazanfari and Franck Guy and Don Lyaruu and {van Ruijve}, Leo and Pamela DenBesten and Stefania Martignon and Gina Castiblanco and Antonius Bronckers",
year = "2017",
month = "11",
doi = "10.1016/j.archoralbio.2017.07.018",
language = "English",
volume = "83",
pages = "214--221",
journal = "Archives of Oral Biology",
issn = "0003-9969",
publisher = "Elsevier Limited",

}

Jalali, R, Ghazanfari, S, Guy, F, Lyaruu, D, van Ruijve, L, DenBesten, P, Martignon, S, Castiblanco, G & Bronckers, A 2017, 'Mineralization-defects are comparable in fluorotic impacted human teeth and fluorotic mouse incisors', Archives of Oral Biology, vol. 83, pp. 214-221. https://doi.org/10.1016/j.archoralbio.2017.07.018

Mineralization-defects are comparable in fluorotic impacted human teeth and fluorotic mouse incisors. / Jalali, Rozita; Ghazanfari, Samaneh; Guy, Franck ; Lyaruu, Don ; van Ruijve, Leo ; DenBesten, Pamela ; Martignon, Stefania ; Castiblanco, Gina; Bronckers, Antonius .

In: Archives of Oral Biology, Vol. 83, 11.2017, p. 214-221.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Mineralization-defects are comparable in fluorotic impacted human teeth and fluorotic mouse incisors

AU - Jalali, Rozita

AU - Ghazanfari, Samaneh

AU - Guy, Franck

AU - Lyaruu, Don

AU - van Ruijve, Leo

AU - DenBesten, Pamela

AU - Martignon, Stefania

AU - Castiblanco, Gina

AU - Bronckers, Antonius

PY - 2017/11

Y1 - 2017/11

N2 - Objective: Fluoride excess of 0.05-0.07 mg F/kg bw/day in water or food additives like salt is the principal cause of endemic dental fluorosis. How fluoride causes these defects is not clear yet. Recent studies in rodents suggest that development of enamel fluorosis is associated with insufficient neutralization of protons released during the formation of hypermineralized lines.Design: Here we examined whether hypermineralization could also be assessed by MicroCT in developing molar enamel of humans exposed to fluoride.Result Micro-CT analysis of hypomineralized enamel from human fluorotic molars graded by the Thylstrup Fejerskov(TF) Index as showed weak hypermineralized lines and hypermineralized patches not seen in TF-I/II grade enamel. The mesio-distal sides of these molar teeth were significantly smaller (similar to 18%, p = 0.02) than in TF-I/II teeth.Conclusion: The patterns of changes observed in human fluorotic teeth were similar to those in fluorotic rodent incisors. The data are consistent with the hypothesis that also in developing human teeth fluoride-stimulated local acidification of enamel could be a mechanism for developing fluorotic enamel.

AB - Objective: Fluoride excess of 0.05-0.07 mg F/kg bw/day in water or food additives like salt is the principal cause of endemic dental fluorosis. How fluoride causes these defects is not clear yet. Recent studies in rodents suggest that development of enamel fluorosis is associated with insufficient neutralization of protons released during the formation of hypermineralized lines.Design: Here we examined whether hypermineralization could also be assessed by MicroCT in developing molar enamel of humans exposed to fluoride.Result Micro-CT analysis of hypomineralized enamel from human fluorotic molars graded by the Thylstrup Fejerskov(TF) Index as showed weak hypermineralized lines and hypermineralized patches not seen in TF-I/II grade enamel. The mesio-distal sides of these molar teeth were significantly smaller (similar to 18%, p = 0.02) than in TF-I/II teeth.Conclusion: The patterns of changes observed in human fluorotic teeth were similar to those in fluorotic rodent incisors. The data are consistent with the hypothesis that also in developing human teeth fluoride-stimulated local acidification of enamel could be a mechanism for developing fluorotic enamel.

KW - AMELOBLASTS

KW - AMELOGENESIS

KW - DENTAL FLUOROSIS

KW - Dental fluorosis

KW - ENAMEL FLUOROSIS

KW - EXPOSURE

KW - Enamel development

KW - FLUORIDE

KW - Hypermineralization

KW - Hypomineralization

KW - MODULATION

KW - ORGAN-CULTURE

KW - RAT INCISORS

KW - SECRETORY-STAGE

U2 - 10.1016/j.archoralbio.2017.07.018

DO - 10.1016/j.archoralbio.2017.07.018

M3 - Article

VL - 83

SP - 214

EP - 221

JO - Archives of Oral Biology

JF - Archives of Oral Biology

SN - 0003-9969

ER -