Metal-Chelating Self-Assembling Peptide Nanofiber Scaffolds for Modulation of Neuronal Cell Behavior

K. Dayob; A. Zengin; R. Garifullin; M.O. Guler; T.I. Abdullin; A. Yergeshov; D.V. Salakhieva; H.H. Cong; M. Zoughaib

doi:10.3390/mi14040883

Metal-Chelating Self-Assembling Peptide Nanofiber Scaffolds for Modulation of Neuronal Cell Behavior

K. Dayob, A. Zengin, R. Garifullin^*, M.O. Guler, T.I. Abdullin, A. Yergeshov, D.V. Salakhieva, H.H. Cong, M. Zoughaib^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Synthetic peptides are promising structural and functional components of bioactive and tissue-engineering scaffolds. Here, we demonstrate the design of self-assembling nanofiber scaffolds based on peptide amphiphile (PA) molecules containing multi-functional histidine residues with trace metal (TM) coordination ability. The self-assembly of PAs and characteristics of PA nanofiber scaffolds along with their interaction with Zn, Cu, and Mn essential microelements were studied. The effects of TM-activated PA scaffolds on mammalian cell behavior, reactive oxygen species (ROS), and glutathione levels were shown. The study reveals the ability of these scaffolds to modulate adhesion, proliferation, and morphological differentiation of neuronal PC-12 cells, suggesting a particular role of Mn(II) in cell-matrix interaction and neuritogenesis. The results provide a proof-of-concept for the development of histidine-functionalized peptide nanofiber scaffolds activated with ROS- and cell-modulating TMs to induce regenerative responses.

Original language	English
Article number	883
Number of pages	19
Journal	Micromachines
Volume	14
Issue number	4
DOIs	https://doi.org/10.3390/mi14040883
Publication status	Published - 1 Apr 2023

Keywords

peptide amphiphiles
self-assembly
nanofiber scaffold
histidine
trace metals
reactive oxygen species
regenerative responses
neuronal differentiation
BIOACTIVE GLASS SCAFFOLDS
MANGANESE
AMPHIPHILES
DIFFERENTIATION
OUTGROWTH
BIOLOGY
RELEASE
BINDING
PROTEIN
AGENTS

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Cite this

@article{eb520961455541d68670b314bc0783ce,

title = "Metal-Chelating Self-Assembling Peptide Nanofiber Scaffolds for Modulation of Neuronal Cell Behavior",

abstract = "Synthetic peptides are promising structural and functional components of bioactive and tissue-engineering scaffolds. Here, we demonstrate the design of self-assembling nanofiber scaffolds based on peptide amphiphile (PA) molecules containing multi-functional histidine residues with trace metal (TM) coordination ability. The self-assembly of PAs and characteristics of PA nanofiber scaffolds along with their interaction with Zn, Cu, and Mn essential microelements were studied. The effects of TM-activated PA scaffolds on mammalian cell behavior, reactive oxygen species (ROS), and glutathione levels were shown. The study reveals the ability of these scaffolds to modulate adhesion, proliferation, and morphological differentiation of neuronal PC-12 cells, suggesting a particular role of Mn(II) in cell-matrix interaction and neuritogenesis. The results provide a proof-of-concept for the development of histidine-functionalized peptide nanofiber scaffolds activated with ROS- and cell-modulating TMs to induce regenerative responses.",

keywords = "peptide amphiphiles, self-assembly, nanofiber scaffold, histidine, trace metals, reactive oxygen species, regenerative responses, neuronal differentiation, BIOACTIVE GLASS SCAFFOLDS, MANGANESE, AMPHIPHILES, DIFFERENTIATION, OUTGROWTH, BIOLOGY, RELEASE, BINDING, PROTEIN, AGENTS",

author = "K. Dayob and A. Zengin and R. Garifullin and M.O. Guler and T.I. Abdullin and A. Yergeshov and D.V. Salakhieva and H.H. Cong and M. Zoughaib",

note = "Funding Information: The authors acknowledge the support of the subsidy allocated to Kazan Federal University (KFU) for the state assignment in the sphere of scientific activities (project FZSM-2022-0020) (design of nanofiber scaffolds). The authors thank Amina G. Daminova and Alexey Rogov (Interdisciplinary Center for Analytical Microscopy, KFU) for the microscopy analyses, Vitaly Chasov for ITC analysis, and Alper D. Ozkan for AFM imaging. This work is part of the Kazan Federal University strategic academic leadership program. H.H. Cong acknowledges the support of VAST project QTRU01.02/21-22. Funding Information: This study was funded by the Russian Science Foundation according to the research project № 22-74-00082, https://rscf.ru/en/project/22-74-00082/ (accessed on 1 August 2022) (in vitro study of peptide matrices). Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = apr,

day = "1",

doi = "10.3390/mi14040883",

language = "English",

volume = "14",

journal = "Micromachines",

issn = "2072-666X",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

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TY - JOUR

T1 - Metal-Chelating Self-Assembling Peptide Nanofiber Scaffolds for Modulation of Neuronal Cell Behavior

AU - Dayob, K.

AU - Zengin, A.

AU - Garifullin, R.

AU - Guler, M.O.

AU - Abdullin, T.I.

AU - Yergeshov, A.

AU - Salakhieva, D.V.

AU - Cong, H.H.

AU - Zoughaib, M.

N1 - Funding Information: The authors acknowledge the support of the subsidy allocated to Kazan Federal University (KFU) for the state assignment in the sphere of scientific activities (project FZSM-2022-0020) (design of nanofiber scaffolds). The authors thank Amina G. Daminova and Alexey Rogov (Interdisciplinary Center for Analytical Microscopy, KFU) for the microscopy analyses, Vitaly Chasov for ITC analysis, and Alper D. Ozkan for AFM imaging. This work is part of the Kazan Federal University strategic academic leadership program. H.H. Cong acknowledges the support of VAST project QTRU01.02/21-22. Funding Information: This study was funded by the Russian Science Foundation according to the research project № 22-74-00082, https://rscf.ru/en/project/22-74-00082/ (accessed on 1 August 2022) (in vitro study of peptide matrices). Publisher Copyright: © 2023 by the authors.

PY - 2023/4/1

Y1 - 2023/4/1

N2 - Synthetic peptides are promising structural and functional components of bioactive and tissue-engineering scaffolds. Here, we demonstrate the design of self-assembling nanofiber scaffolds based on peptide amphiphile (PA) molecules containing multi-functional histidine residues with trace metal (TM) coordination ability. The self-assembly of PAs and characteristics of PA nanofiber scaffolds along with their interaction with Zn, Cu, and Mn essential microelements were studied. The effects of TM-activated PA scaffolds on mammalian cell behavior, reactive oxygen species (ROS), and glutathione levels were shown. The study reveals the ability of these scaffolds to modulate adhesion, proliferation, and morphological differentiation of neuronal PC-12 cells, suggesting a particular role of Mn(II) in cell-matrix interaction and neuritogenesis. The results provide a proof-of-concept for the development of histidine-functionalized peptide nanofiber scaffolds activated with ROS- and cell-modulating TMs to induce regenerative responses.

AB - Synthetic peptides are promising structural and functional components of bioactive and tissue-engineering scaffolds. Here, we demonstrate the design of self-assembling nanofiber scaffolds based on peptide amphiphile (PA) molecules containing multi-functional histidine residues with trace metal (TM) coordination ability. The self-assembly of PAs and characteristics of PA nanofiber scaffolds along with their interaction with Zn, Cu, and Mn essential microelements were studied. The effects of TM-activated PA scaffolds on mammalian cell behavior, reactive oxygen species (ROS), and glutathione levels were shown. The study reveals the ability of these scaffolds to modulate adhesion, proliferation, and morphological differentiation of neuronal PC-12 cells, suggesting a particular role of Mn(II) in cell-matrix interaction and neuritogenesis. The results provide a proof-of-concept for the development of histidine-functionalized peptide nanofiber scaffolds activated with ROS- and cell-modulating TMs to induce regenerative responses.

KW - peptide amphiphiles

KW - self-assembly

KW - nanofiber scaffold

KW - histidine

KW - trace metals

KW - reactive oxygen species

KW - regenerative responses

KW - neuronal differentiation

KW - BIOACTIVE GLASS SCAFFOLDS

KW - MANGANESE

KW - AMPHIPHILES

KW - DIFFERENTIATION

KW - OUTGROWTH

KW - BIOLOGY

KW - RELEASE

KW - BINDING

KW - PROTEIN

KW - AGENTS

U2 - 10.3390/mi14040883

DO - 10.3390/mi14040883

M3 - Article

C2 - 37421116

SN - 2072-666X

VL - 14

JO - Micromachines

JF - Micromachines

IS - 4

M1 - 883

ER -