TY - JOUR
T1 - Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) reveals potential lipid markers between infrapatellar fat pad biopsies of osteoarthritis and cartilage defect patients
AU - Haartmans, Mirella J.J.
AU - Claes, Britt S.R.
AU - Eijkel, Gert B.
AU - Emanuel, Kaj S.
AU - Tuijthof, Gabrielle J.M.
AU - Heeren, Ron M.A.
AU - Emans, Pieter J.
AU - Cillero-Pastor, Berta
N1 - Funding Information:
This work was supported by the Dutch Research Council (NWO) domain Applied and Engineering Sciences (Stichting voor de Technische Wetenschappen (Foundation for Technical Sciences, STW) P15-23; William Hunter Revisited). The research conducted at the M4i institute was funded by the Dutch Province of Limburg through the LINK (Limburg INvesteert in haar Kenniseconomie) program.
Funding Information:
Tissue collection and logistics were supported by the orthopedic clinical trial center and laboratory for experimental orthopedics, supported by the LLP14 grant of the Dutch Arthritis Society (ReumaNederland).
Publisher Copyright:
© 2023, The Author(s).
PY - 2023/10
Y1 - 2023/10
N2 - The incidence of osteoarthritis (OA) has been expected to increase due to an aging population, as well as an increased incidence of intra-articular (osteo-) chondral damage. Lipids have already been shown to be involved in the inflammatory process of OA. This study aims at revealing region-specific lipid profiles of the infrapatellar fat pad (IPFP) of OA or cartilage defect patients by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), which could be used as biomarkers for early OA detection. A higher presence of phospholipids was found in OA patients compared with cartilage defect patients. In addition, a higher abundance of ether-linked phosphatidylethanolamines (PE O-s) containing arachidonic acid was specifically found in OA patients compared with cartilage defect patients. These lipids were mainly found in the connective tissue of the IPFP. Specific lipid species were associated to OA patients compared with cartilage defect patients. PE O-s have been suggested as possible biomarkers for OA. As these were found more abundantly in the connective tissue, the IPFP’s intra-tissue heterogeneity might play an important role in biomarker discovery, implying that the amount of fibrous tissue is associated with OA. Graphical Abstract: [Figure not available: see fulltext.].
AB - The incidence of osteoarthritis (OA) has been expected to increase due to an aging population, as well as an increased incidence of intra-articular (osteo-) chondral damage. Lipids have already been shown to be involved in the inflammatory process of OA. This study aims at revealing region-specific lipid profiles of the infrapatellar fat pad (IPFP) of OA or cartilage defect patients by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), which could be used as biomarkers for early OA detection. A higher presence of phospholipids was found in OA patients compared with cartilage defect patients. In addition, a higher abundance of ether-linked phosphatidylethanolamines (PE O-s) containing arachidonic acid was specifically found in OA patients compared with cartilage defect patients. These lipids were mainly found in the connective tissue of the IPFP. Specific lipid species were associated to OA patients compared with cartilage defect patients. PE O-s have been suggested as possible biomarkers for OA. As these were found more abundantly in the connective tissue, the IPFP’s intra-tissue heterogeneity might play an important role in biomarker discovery, implying that the amount of fibrous tissue is associated with OA. Graphical Abstract: [Figure not available: see fulltext.].
KW - Biomarkers
KW - Cartilage regeneration
KW - Hoffa’s fat pad
KW - Lipids
KW - Mass spectrometry imaging
KW - Total knee arthroplasty
U2 - 10.1007/s00216-023-04871-9
DO - 10.1007/s00216-023-04871-9
M3 - Article
C2 - 37505238
SN - 1618-2642
VL - 415
SP - 5997
EP - 6007
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 24
ER -