Lesional and perilesional tissue characterization by automated image processing in a novel gyrencephalic animal model of peracute intracerebral hemorrhage

Johannes Boltze, Fabienne Ferrara, Atticus H. Hainsworth, Leslie R. Bridges, Marietta Zille, Donald Lobsien, Henryk Barthel, Damian D. McLeod, Felix Graesser, Soeren Pietsch, Ann-Kathrin Schatzl, Antje Y. Dreyer, Bjorn Nitzsche*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Web of Science)

Abstract

Intracerebral hemorrhage (ICH) is an important stroke subtype, but preclinical research is limited by a lack of translational animal models. Large animal models are useful to comparatively investigate key pathophysiological parameters in human ICH. To (i) establish an acute model of moderate ICH in adult sheep and (ii) an advanced neuroimage processing pipeline for automatic brain tissue and hemorrhagic lesion determination; 14 adult sheep were assigned for stereotactically induced ICH into cerebral white matter under physiological monitoring. Six hours after ICH neuroimaging using 1.5T MRI including structural as well as perfusion and diffusion, weighted imaging was performed before scarification and subsequent neuropathological investigation including immunohistological staining. Controlled, stereotactic application of autologous blood caused a space-occupying intracerebral hematoma of moderate severity, predominantly affecting white matter at 5 h post-injection. Neuroimage post-processing including lesion probability maps enabled automatic quantification of structural alterations including perilesional diffusion and perfusion restrictions. Neuropathological and immunohistological investigation confirmed perilesional vacuolation, axonal damage, and perivascular blood as seen after human ICH. The model and imaging platform reflects key aspects of human ICH and enables future translational research on hematoma expansion/evacuation, white matter changes, hematoma evacuation, and other aspects.

Original languageEnglish
Pages (from-to)2521-2535
Number of pages15
JournalJournal of Cerebral Blood Flow and Metabolism
Volume39
Issue number12
DOIs
Publication statusPublished - Dec 2019

Keywords

  • Brain
  • CEREBRAL PERFUSION
  • DISEASE
  • GLOBAL BURDEN
  • HEMATOMA VOLUME
  • INJURY
  • OUTCOMES
  • PERIHEMATOMAL EDEMA
  • PRESSURE
  • SUBARACHNOID HEMORRHAGE
  • SYSTEMATIC ANALYSIS
  • hemorrhage
  • large animal
  • perfusion
  • segmentation
  • 1ST

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