Abstract
Sample preparation is rapidly improving to fulfill the need for faster and more environmentally friendly alternatives. In this respect, ionic liquid-based dispersive liquid-liquid microextraction (IL-DLLME) is an interesting technique. However, it has not yet been evaluated for the analysis of postmortem samples, which are frequently analyzed in forensic toxicology. This study investigates the applicability of IL-DLLME coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS), for the analysis of benzodiazepines in postmortem blood of 11 forensic cases. The method was compared with a validated solid-phase extraction (SPE) method. Bland-Altman analysis was performed on 24 benzodiazepine measurements. Both methods gave comparable results, except for flurazepam and temazepam (>55% difference). A feasible explanation is high postmortem matrix variability that was not considered during IL-DLLME validation experiments. Another issue could be the use of a single nondeuterated SPE internal standard. Overall, IL-DLLME has proven its usability for the analysis of postmortem blood.
Original language | English |
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Pages (from-to) | 1875-1879 |
Number of pages | 5 |
Journal | Journal of Forensic Sciences |
Volume | 63 |
Issue number | 6 |
DOIs | |
Publication status | Published - Nov 2018 |
Externally published | Yes |
Keywords
- Benzodiazepines/blood
- Chromatography, Liquid
- Forensic Toxicology/methods
- Humans
- Hypnotics and Sedatives/blood
- Ionic Liquids
- Limit of Detection
- Liquid Phase Microextraction/methods
- Solid Phase Extraction
- Tandem Mass Spectrometry