@article{56b61907d69e4daf90acb51d019afd78,
title = "Influence of Different Cell Types and Sources on Pre-Vascularisation in Fibrin and Agarose-Collagen Gels",
abstract = "Vascularisation is essential for the development of tailored, tissue-engineered organs and tissues due to diffusion limits of nutrients and the lack of the necessary connection to the cardiovascular system. To pre-vascularize, endothelial cells and supporting cells can be embedded in the scaffold to foster an adequate nutrient and oxygen supply after transplantation. This technique is applied for tissue engineering of various tissues, but there have been few studies on the use of different cell types or cells sources. We compare the effect of supporting cells from different sources on vascularisation. Fibrin gels and agarose-collagen hydrogels were used as scaffolds. The supporting cells were primary human dermal fibroblasts (HDFs), human nasal fibroblasts (HNFs), human mesenchymal stem cells from umbilical cord's Wharton's jelly (WJ MSCs), adipose-derived MSCs (AD MSCs) and femoral bone marrow-derived MSCs (BM MSCs). The tissue constructs were incubated for 14 days and analyzed by two-photon laser scanning microscopy. Vascularisation was supported by all cell types, forming branched networks of tubular vascular structures in both hydrogels. In general, fibrin gels present a higher angiogenic promoting environment compared to agarose-collagen hydrogels and fibroblasts show a high angiogenic potential in co-culture with endothelial cells. In agarose-collagen hydrogels, vascular structures supported by AD MSCs were comparable to our HDF control in terms of volume, area and length. BM MSCs formed a homogeneous network of smaller structures in both hydrogels. This study provides data toward understanding the pre-vascularisation properties of different supporting cell types and sources for tissue engineering of different organs and tissues.",
keywords = "3D cell culture, co-cultures, hydrogels, human mesenchymal stem cells, vascularisation, MESENCHYMAL STEM-CELLS, ENDOTHELIAL-CELLS, STROMAL CELLS, ANGIOGENESIS, FIBROBLASTS, SCAFFOLD, THERAPY",
author = "Caroline Kniebs and Franziska Kreimendahl and Marius Koepf and Horst Fischer and Stefan Jockenhoevel and Thiebes, {Anja Lena}",
note = "Funding Information: The authors would like to thank the RWTH Aachen University Hospital for providing human tissue for cell isolation: the Department of Gynaecology and Perinatal Medicine (Prof. Dr. Stickeler) for providing umbilical cords, the Department for Otorhinolaryngology and Plastic Surgery of the Head and Throat (Prof. Dr. Westhofen and Dr. Ilgner) for providing nose shell tissue, the Orthopaedic Clinic for providing femoral heads (PD Dr. Rath) and the Clinic for Oral and Maxillofacial Surgery (Prof. Dr. H{\"o}lzle and PD Dr. Lethaus) for providing skin and fat tissue for this study. We thank Dr. Richard Twyman for editing this paper, Felicitas Kempf and Bernhard L{\"u}ttgenau for the statistical and mathematical assistance. This work was supported by the Core Facility {\textquoteleft}{\textquoteleft}Two-Photon Imaging{\textquoteright}{\textquoteright} [Interdisciplinary Centre for Clinical Research (IZKF Aachen)] within the Faculty of Medicine at RWTH Aachen University and by the Flow Cytometry Facility, a core facility of the Interdisciplinary Center for Clinical Research (IZKF) Aachen within the Faculty of Medicine at RWTH Aachen University. We thank Marie Hauser (BioTex) for her support with the FACS analysis. Funding Information: This study was supported by Deutsche Forschungsgemeinschaft (Grant No. JO 764/4-2 and FI 975/23-2). The authors would like to thank the RWTH Aachen University Hospital for providing human tissue for cell isolation: the Department of Gynaecology and Perinatal Medicine (Prof. Dr. Stickeler) for providing umbilical cords, the Department for Otorhinolaryngology and Plastic Surgery of the Head and Throat (Prof. Dr. Westhofen and Dr. Ilgner) for providing nose shell tissue, the Orthopaedic Clinic for providing femoral heads (PD Dr. Rath) and the Clinic for Oral and Maxillofacial Surgery (Prof. Dr. H?lzle and PD Dr. Lethaus) for providing skin and fat tissue for this study. We thank Dr. Richard Twyman for editing this paper, Felicitas Kempf and Bernhard?L?ttgenau?for the statistical and mathematical assistance. This work was supported by the Core Facility ??Two-Photon Imaging?? [Interdisciplinary Centre for Clinical Research (IZKF Aachen)] within the Faculty of Medicine at RWTH Aachen University and by the Flow Cytometry Facility, a core facility of the Interdisciplinary Center for Clinical Research (IZKF) Aachen within the Faculty of Medicine at RWTH Aachen University. We thank Marie Hauser (BioTex) for her support with the FACS analysis. Publisher Copyright: {\textcopyright} 2019, {\textcopyright} 2019 The Author(s). Published with license by Taylor & Francis Group, LLC.",
year = "2020",
month = jan,
day = "2",
doi = "10.1080/15476278.2019.1697597",
language = "English",
volume = "16",
pages = "14--26",
journal = "Organogenesis",
issn = "1547-6278",
publisher = "Routledge/Taylor & Francis Group",
number = "1",
}