In vitro mucin degradation and paracellular permeability by fecal water from Crohn's disease patients

Heike Ef Becker; Nader Kameli; Alice Rustichelli; Britt Am Heijnens; Frank Rm Stassen; John Penders; Daisy Mae Jonkers

doi:10.2217/fmb-2022-0265

In vitro mucin degradation and paracellular permeability by fecal water from Crohn's disease patients

Heike Ef Becker, Nader Kameli, Alice Rustichelli, Britt Am Heijnens, Frank Rm Stassen, John Penders, Daisy Mae Jonkers^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

This study aimed to examine the impact of fecal water (FW) of active and remissive Crohn's disease (CD) patients on mucin degradation and epithelial barrier function. FW and bacterial membrane vesicles (MVs) were isolated from fresh fecal samples of six healthy controls (HCs) and 12 CD patients. Bacterial composition was determined by 16S rRNA gene amplicon sequencing. FW-induced mucin degradation was higher in CD samples versus HC (p < 0.01), but not associated with specific bacterial genera. FW of three remissive samples decreased transepithelial electrical resistance in Caco-2 cells by 78-87% (p < 0.001). MVs did not induce barrier alterations. The higher mucin-degradation capacity of CD-derived FW might suggest contributions of microbial products to CD pathophysiology.

Original language	English
Pages (from-to)	335-347
Number of pages	13
Journal	Future Microbiology
Volume	19
DOIs	https://doi.org/10.2217/fmb-2022-0265
Publication status	Published - 12 Mar 2024

Keywords

Crohn's disease
fecal microbial secretome
intestinal epithelial barrier
mucin

Access to Document

10.2217/fmb-2022-0265

Cite this

@article{ee6fccd5f6f942e58535f1f3f358d836,

title = "In vitro mucin degradation and paracellular permeability by fecal water from Crohn's disease patients",

abstract = "This study aimed to examine the impact of fecal water (FW) of active and remissive Crohn's disease (CD) patients on mucin degradation and epithelial barrier function. FW and bacterial membrane vesicles (MVs) were isolated from fresh fecal samples of six healthy controls (HCs) and 12 CD patients. Bacterial composition was determined by 16S rRNA gene amplicon sequencing. FW-induced mucin degradation was higher in CD samples versus HC (p < 0.01), but not associated with specific bacterial genera. FW of three remissive samples decreased transepithelial electrical resistance in Caco-2 cells by 78-87% (p < 0.001). MVs did not induce barrier alterations. The higher mucin-degradation capacity of CD-derived FW might suggest contributions of microbial products to CD pathophysiology.",

keywords = "Crohn's disease, fecal microbial secretome, intestinal epithelial barrier, mucin",

author = "Becker, {Heike Ef} and Nader Kameli and Alice Rustichelli and Heijnens, {Britt Am} and Stassen, {Frank Rm} and John Penders and Jonkers, {Daisy Mae}",

year = "2024",

month = mar,

day = "12",

doi = "10.2217/fmb-2022-0265",

language = "English",

volume = "19",

pages = "335--347",

journal = "Future Microbiology",

issn = "1746-0913",

publisher = "Future Medicine Ltd.",

}

TY - JOUR

T1 - In vitro mucin degradation and paracellular permeability by fecal water from Crohn's disease patients

AU - Becker, Heike Ef

AU - Kameli, Nader

AU - Rustichelli, Alice

AU - Heijnens, Britt Am

AU - Stassen, Frank Rm

AU - Penders, John

AU - Jonkers, Daisy Mae

PY - 2024/3/12

Y1 - 2024/3/12

N2 - This study aimed to examine the impact of fecal water (FW) of active and remissive Crohn's disease (CD) patients on mucin degradation and epithelial barrier function. FW and bacterial membrane vesicles (MVs) were isolated from fresh fecal samples of six healthy controls (HCs) and 12 CD patients. Bacterial composition was determined by 16S rRNA gene amplicon sequencing. FW-induced mucin degradation was higher in CD samples versus HC (p < 0.01), but not associated with specific bacterial genera. FW of three remissive samples decreased transepithelial electrical resistance in Caco-2 cells by 78-87% (p < 0.001). MVs did not induce barrier alterations. The higher mucin-degradation capacity of CD-derived FW might suggest contributions of microbial products to CD pathophysiology.

AB - This study aimed to examine the impact of fecal water (FW) of active and remissive Crohn's disease (CD) patients on mucin degradation and epithelial barrier function. FW and bacterial membrane vesicles (MVs) were isolated from fresh fecal samples of six healthy controls (HCs) and 12 CD patients. Bacterial composition was determined by 16S rRNA gene amplicon sequencing. FW-induced mucin degradation was higher in CD samples versus HC (p < 0.01), but not associated with specific bacterial genera. FW of three remissive samples decreased transepithelial electrical resistance in Caco-2 cells by 78-87% (p < 0.001). MVs did not induce barrier alterations. The higher mucin-degradation capacity of CD-derived FW might suggest contributions of microbial products to CD pathophysiology.

KW - Crohn's disease

KW - fecal microbial secretome

KW - intestinal epithelial barrier

KW - mucin

U2 - 10.2217/fmb-2022-0265

DO - 10.2217/fmb-2022-0265

M3 - Article

SN - 1746-0913

VL - 19

SP - 335

EP - 347

JO - Future Microbiology

JF - Future Microbiology

ER -