In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels

Matilde Bongio; M. Reza Nejadnik; Z. Tahmasebi Birgani; Pamela Habibovic; Lucas A. Kinard; F. Kurtis Kasper; Antonios G. Mikos; John A. Jansen; Sander C. G. Leeuwenburgh; Jeroen J. J. P. van den Beucken

doi:10.1002/mabi.201200474

In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels

Matilde Bongio, M. Reza Nejadnik, Z. Tahmasebi Birgani, Pamela Habibovic, Lucas A. Kinard, F. Kurtis Kasper, Antonios G. Mikos, John A. Jansen, Sander C. G. Leeuwenburgh, Jeroen J. J. P. van den Beucken^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10mg center dot ml-1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10mM calcium glycerophosphate (Ca-GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (approximate to 5-20 mu m) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.

Original language	English
Pages (from-to)	777-788
Number of pages	12
Journal	Macromolecular Bioscience
Volume	13
Issue number	6
DOIs	https://doi.org/10.1002/mabi.201200474
Publication status	Published - Jun 2013
Externally published	Yes

Keywords

alkaline phosphatase
biomineralization
bone substitutes
hydrogels
in vivo
ALKALINE-PHOSPHATASE
HEXOSEPHOSPHORIC ESTERS
TISSUE-RESPONSE
BONE
MINERALIZATION
CALCIUM
DIFFERENTIATION
CALCIFICATION
COMPOSITES
OSTEOBLASTS

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10.1002/mabi.201200474

Cite this

@article{9c50ce7d48de47a1935256ca27cd8227,

title = "In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels",

abstract = "The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10mg center dot ml-1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10mM calcium glycerophosphate (Ca-GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (approximate to 5-20 mu m) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.",

keywords = "alkaline phosphatase, biomineralization, bone substitutes, hydrogels, in vivo, ALKALINE-PHOSPHATASE, HEXOSEPHOSPHORIC ESTERS, TISSUE-RESPONSE, BONE, MINERALIZATION, CALCIUM, DIFFERENTIATION, CALCIFICATION, COMPOSITES, OSTEOBLASTS",

author = "Matilde Bongio and Nejadnik, {M. Reza} and Birgani, {Z. Tahmasebi} and Pamela Habibovic and Kinard, {Lucas A.} and Kasper, {F. Kurtis} and Mikos, {Antonios G.} and Jansen, {John A.} and Leeuwenburgh, {Sander C. G.} and {van den Beucken}, {Jeroen J. J. P.}",

year = "2013",

month = jun,

doi = "10.1002/mabi.201200474",

language = "English",

volume = "13",

pages = "777--788",

journal = "Macromolecular Bioscience",

issn = "1616-5187",

publisher = "John Wiley & Sons Inc.",

number = "6",

}

TY - JOUR

T1 - In Vitro and In Vivo Enzyme-Mediated Biomineralization of Oligo(poly(ethylene glycol) Fumarate Hydrogels

AU - Bongio, Matilde

AU - Nejadnik, M. Reza

AU - Birgani, Z. Tahmasebi

AU - Habibovic, Pamela

AU - Kinard, Lucas A.

AU - Kasper, F. Kurtis

AU - Mikos, Antonios G.

AU - Jansen, John A.

AU - Leeuwenburgh, Sander C. G.

AU - van den Beucken, Jeroen J. J. P.

PY - 2013/6

Y1 - 2013/6

N2 - The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10mg center dot ml-1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10mM calcium glycerophosphate (Ca-GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (approximate to 5-20 mu m) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.

AB - The enzyme alkaline phosphatase (ALP) is added at different concentrations (i.e., 0, 2.5, and 10mg center dot ml-1) to oligo(poly(ethylene glycol)fumarate) (OPF) hydrogels. The scaffolds are either incubated in 10mM calcium glycerophosphate (Ca-GP) solution for 2 weeks or implanted in a rat subcutaneous model for 4 weeks. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and alizarin red staining show a strong ability to form minerals exclusively in ALP-containing hydrogels in vitro. Additionally, the calcium content increases with increasing ALP concentration. Similarly, only ALP-containing hydrogels induce mineralization in vivo. Specifically, small (approximate to 5-20 mu m) mineral deposits are observed at the periphery of the hydrogels near the dermis/scaffold interface using Von Kossa and alizarin red staining.

KW - alkaline phosphatase

KW - biomineralization

KW - bone substitutes

KW - hydrogels

KW - in vivo

KW - ALKALINE-PHOSPHATASE

KW - HEXOSEPHOSPHORIC ESTERS

KW - TISSUE-RESPONSE

KW - BONE

KW - MINERALIZATION

KW - CALCIUM

KW - DIFFERENTIATION

KW - CALCIFICATION

KW - COMPOSITES

KW - OSTEOBLASTS

U2 - 10.1002/mabi.201200474

DO - 10.1002/mabi.201200474

M3 - Article

SN - 1616-5187

VL - 13

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JO - Macromolecular Bioscience

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ER -