Implementation of an Automated Manufacturing Platform for Engineering of Functional Osteochondral Implants

J. Krieger; B. Niessing; N. Konig; C. Mota; V. La Pointe; S. Van Rijt; D. Kondro; M. Hiatt; B. Viellerobe; B. Brisson; M. Marechal; L. Geris; F.P. Luyten; I. Papantoniou; R.H. Schmitt

doi:10.1016/j.procir.2022.06.008

Implementation of an Automated Manufacturing Platform for Engineering of Functional Osteochondral Implants

J. Krieger^*, B. Niessing, N. Konig, C. Mota, V. La Pointe, S. Van Rijt, D. Kondro, M. Hiatt, B. Viellerobe, B. Brisson, M. Marechal, L. Geris, F.P. Luyten, I. Papantoniou, R.H. Schmitt

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceeding › Academic › peer-review

Abstract

The EU Horizon 2020 project "JointPromise" proposes the development and implementation of an end-to-end automated production platform for three-dimensional joint implants, paving the way for tissue-engineered implants able to regenerate deep osteochondral defects. Currently, the manufacturing pipeline consists in manual production processes for microtissue cultivation, harvest and bioassembly into larger implants. In the conceptualizing stage of this project, the manual processes were translated into standard operating protocols (SOPs) and process design criteria like material flow and throughput as well as technical specifications of laboratory devices for an automated performance were elaborated.Spheroid-based implants provide a novel approach in tissue engineering by aggregating progenitor cells into potent microtissues. After the differentiation of cartilaginous microtissues, functional joint implants are assembled via 3D bioprinting to match the complex structural organization of native cartilage tissue. The "JointPromise" platform includes suitable devices for cell and microtissue cultivation, harvest and implant production as well as quality control in an overall layout consisting of according pipetting units, incubator, centrifuge, bioprinter and high-speed microscope. After initiating the platform build-up, a control software for process controlling and monitoring during cell seeding, cultivation and harvest is implemented. Clinical feasibility and efficacy of osteochondral defect regeneration by the produced joint implants will subsequently be proven in large animal models. (C) 2022 The Authors. Published by Elsevier B.V.

Original language	English
Title of host publication	V CIRP CONFERENCE ON BIOMANUFACTURING
Publisher	Elsevier Inc.
Pages	32-35
Number of pages	4
Volume	110
DOIs	https://doi.org/10.1016/j.procir.2022.06.008
Publication status	Published - 2022
Event	5th V CIRP Conference on BioManufacturing - Calabria, Italy Duration: 22 Jun 2022 → 24 Jun 2022 Conference number: 5

Publication series

Series	Procedia CIRP
Volume	110
ISSN	2212-8271

Conference

Conference	5th V CIRP Conference on BioManufacturing
Abbreviated title	Cirp BioM 2022
Country/Territory	Italy
City	Calabria
Period	22/06/22 → 24/06/22

Keywords

Automation
Osteoarthritis
Tissue Engineering
Bioprinting
Stem Cells
Regenerative Medicine

Access to Document

10.1016/j.procir.2022.06.008Licence: CC BY-NC-ND

Cite this

Krieger, J., Niessing, B., Konig, N., Mota, C., La Pointe, V., Van Rijt, S., Kondro, D., Hiatt, M., Viellerobe, B., Brisson, B., Marechal, M., Geris, L., Luyten, F. P., Papantoniou, I., & Schmitt, R. H. (2022). Implementation of an Automated Manufacturing Platform for Engineering of Functional Osteochondral Implants. In V CIRP CONFERENCE ON BIOMANUFACTURING (Vol. 110, pp. 32-35). Elsevier Inc.. https://doi.org/10.1016/j.procir.2022.06.008

@inproceedings{5978efc98382454182dbaffbea30fb31,

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keywords = "Automation, Osteoarthritis, Tissue Engineering, Bioprinting, Stem Cells, Regenerative Medicine",

author = "J. Krieger and B. Niessing and N. Konig and C. Mota and {La Pointe}, V. and {Van Rijt}, S. and D. Kondro and M. Hiatt and B. Viellerobe and B. Brisson and M. Marechal and L. Geris and F.P. Luyten and I. Papantoniou and R.H. Schmitt",

note = "Funding Information: This project received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation programme under grant agreement no 874837. {\textcopyright} 2022 The Authors. Published by ELSEVIER B.V. {\textcopyright} 2022 The Authors. Published by Elsevier B.V. Th 2is0 2is2 aTnhoepAenutahcocress.s Paurbtilcisleh eudndbeyr EthLeSCECV IBEYR-BN.CV-.ND license (https://creativecommons.org/licenses/by-nc-nd/4.0) 1. Introduction of the product range and characteristics manufactured and/or Peer-review under responsibility of the scientific committee of the V CIRP Conference on Biomanufacturing assembled in this system. In this context, the main challenge in Peer-review under responsibility of the scientific committee of the V CIRP Conference on Biomanufacturing KeyDwueords:tAoutomtheation;faOsstteoadrtehveritislo; TpismseunetEngiin neeritnheg; Biodporinmtianign ; SteomfCellsm; Roedgeelnleirnag tivaendMedaicnainelysis is now not only to cope with single cKoemywmuordns:icAauttioomn atioandn;Osaten oarothngritiso; Ting issutereEndngineoefrindgi; giBitoipzraintitionn g; SatendmCellsp; rRoduegencetrsa,tiaveliMmeidtiecd ineproduct range or existing product families, Funding Information: cultivation and harvest is implemented. Clinical feasibility and efficacy of osteochondral defect regeneration by the produced joint implants will KsTuehbyiwss eopqrdruosej:enActltsy sreebmceebpilyvreo;dvDefeunsnigidnninlmagregthfreooadmn; iFtmhaemalEil myuroidodepeneltifsa.nicUnatioinon{\textquoteright}s Horizon 2020 research and innovation programme under grant agreement no 874837. Publisher Copyright: {\textcopyright} 2022 The Authors. Published by Elsevier B.V.; 5th V CIRP Conference on BioManufacturing, Cirp BioM 2022 ; Conference date: 22-06-2022 Through 24-06-2022",

year = "2022",

doi = "10.1016/j.procir.2022.06.008",

language = "English",

volume = "110",

series = "Procedia CIRP",

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Krieger, J, Niessing, B, Konig, N, Mota, C, La Pointe, V, Van Rijt, S, Kondro, D, Hiatt, M, Viellerobe, B, Brisson, B, Marechal, M, Geris, L, Luyten, FP, Papantoniou, I & Schmitt, RH 2022, Implementation of an Automated Manufacturing Platform for Engineering of Functional Osteochondral Implants. in V CIRP CONFERENCE ON BIOMANUFACTURING. vol. 110, Elsevier Inc., Procedia CIRP, vol. 110, pp. 32-35, 5th V CIRP Conference on BioManufacturing, Calabria, Italy, 22/06/22. https://doi.org/10.1016/j.procir.2022.06.008

Implementation of an Automated Manufacturing Platform for Engineering of Functional Osteochondral Implants. / Krieger, J.; Niessing, B.; Konig, N. et al.
V CIRP CONFERENCE ON BIOMANUFACTURING. Vol. 110 Elsevier Inc., 2022. p. 32-35 (Procedia CIRP, Vol. 110).

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceeding › Academic › peer-review

TY - GEN

T1 - Implementation of an Automated Manufacturing Platform for Engineering of Functional Osteochondral Implants

AU - Krieger, J.

AU - Niessing, B.

AU - Konig, N.

AU - Mota, C.

AU - La Pointe, V.

AU - Van Rijt, S.

AU - Kondro, D.

AU - Hiatt, M.

AU - Viellerobe, B.

AU - Brisson, B.

AU - Marechal, M.

AU - Geris, L.

AU - Luyten, F.P.

AU - Papantoniou, I.

AU - Schmitt, R.H.

N1 - Conference code: 5

PY - 2022

Y1 - 2022

N2 - The EU Horizon 2020 project "JointPromise" proposes the development and implementation of an end-to-end automated production platform for three-dimensional joint implants, paving the way for tissue-engineered implants able to regenerate deep osteochondral defects. Currently, the manufacturing pipeline consists in manual production processes for microtissue cultivation, harvest and bioassembly into larger implants. In the conceptualizing stage of this project, the manual processes were translated into standard operating protocols (SOPs) and process design criteria like material flow and throughput as well as technical specifications of laboratory devices for an automated performance were elaborated.Spheroid-based implants provide a novel approach in tissue engineering by aggregating progenitor cells into potent microtissues. After the differentiation of cartilaginous microtissues, functional joint implants are assembled via 3D bioprinting to match the complex structural organization of native cartilage tissue. The "JointPromise" platform includes suitable devices for cell and microtissue cultivation, harvest and implant production as well as quality control in an overall layout consisting of according pipetting units, incubator, centrifuge, bioprinter and high-speed microscope. After initiating the platform build-up, a control software for process controlling and monitoring during cell seeding, cultivation and harvest is implemented. Clinical feasibility and efficacy of osteochondral defect regeneration by the produced joint implants will subsequently be proven in large animal models. (C) 2022 The Authors. Published by Elsevier B.V.

AB - The EU Horizon 2020 project "JointPromise" proposes the development and implementation of an end-to-end automated production platform for three-dimensional joint implants, paving the way for tissue-engineered implants able to regenerate deep osteochondral defects. Currently, the manufacturing pipeline consists in manual production processes for microtissue cultivation, harvest and bioassembly into larger implants. In the conceptualizing stage of this project, the manual processes were translated into standard operating protocols (SOPs) and process design criteria like material flow and throughput as well as technical specifications of laboratory devices for an automated performance were elaborated.Spheroid-based implants provide a novel approach in tissue engineering by aggregating progenitor cells into potent microtissues. After the differentiation of cartilaginous microtissues, functional joint implants are assembled via 3D bioprinting to match the complex structural organization of native cartilage tissue. The "JointPromise" platform includes suitable devices for cell and microtissue cultivation, harvest and implant production as well as quality control in an overall layout consisting of according pipetting units, incubator, centrifuge, bioprinter and high-speed microscope. After initiating the platform build-up, a control software for process controlling and monitoring during cell seeding, cultivation and harvest is implemented. Clinical feasibility and efficacy of osteochondral defect regeneration by the produced joint implants will subsequently be proven in large animal models. (C) 2022 The Authors. Published by Elsevier B.V.

KW - Automation

KW - Osteoarthritis

KW - Tissue Engineering

KW - Bioprinting

KW - Stem Cells

KW - Regenerative Medicine

U2 - 10.1016/j.procir.2022.06.008

DO - 10.1016/j.procir.2022.06.008

M3 - Conference article in proceeding

VL - 110

T3 - Procedia CIRP

SP - 32

EP - 35

BT - V CIRP CONFERENCE ON BIOMANUFACTURING

PB - Elsevier Inc.

T2 - 5th V CIRP Conference on BioManufacturing

Y2 - 22 June 2022 through 24 June 2022

ER -