Histopathological evaluation of a retinoic acid eluting stent in a rabbit iliac artery model

Ioanna Samara; Christos S Katsouras; Arsen Semertzioglou; Athanassios Vratimos; Amalia I Moula; Constantinos A Dimitriou; Michail Theofanis; Triantafyllia Papadimitropoulou; Vasileios Bouratzis; Georgia Karanasiou; Dimitrios Fotiadis; Lampros K Michalis; Anargyros N Moulas

doi:10.1038/s41598-022-16025-5

Histopathological evaluation of a retinoic acid eluting stent in a rabbit iliac artery model

Ioanna Samara, Christos S Katsouras, Arsen Semertzioglou, Athanassios Vratimos, Amalia I Moula, Constantinos A Dimitriou, Michail Theofanis, Triantafyllia Papadimitropoulou, Vasileios Bouratzis, Georgia Karanasiou, Dimitrios Fotiadis, Lampros K Michalis, Anargyros N Moulas^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

This study aimed to evaluate the safety and efficacy of innovative retinoic acid (RA) eluting stents with bioabsorbable polymer. Sixty stents divided in ten groups were implanted in the iliac arteries of 30 rabbits. Two polymers ("A", poly (lactic-co-glycolic acid) and "B", polylactic acid), and three doses ("Low", "Medium" and "High") of RA (groups: AL, AM, AH, BL, BM, BH) were used on cobalt chromium stents (Rontis Corporation), one group of bare stent (C), one group (D) of Everolimus eluting stent (Xience-Pro, Abbot Vascular), and two groups of Rontis Everolimus eluting stents coated with polymer A (EA) and B (EB). Treated arteries were explanted after 4 weeks, processed by methyl methacrylate resin and evaluated by histopathology. None of the implanted stents was related with thrombus formation or extensive inflammation. Image analysis showed limited differences between groups regarding area stenosis (BH, D and EB groups had the lower values). Group BH had lower intimal mean thickness than AH (105.1 vs 75.3 μm, p = 0.024). Stents eluting RA, a non-cytotoxic drug, were not related with thrombus formation and had an acceptable degree of stenosis 4 weeks post implantation. RA dose and type of polymer may play role in the biocompatibility of the stents.

Original language	English
Article number	13305
Number of pages	9
Journal	Scientific Reports
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41598-022-16025-5
Publication status	Published - 3 Aug 2022

Keywords

Animals
Constriction, Pathologic/pathology
Drug-Eluting Stents
Everolimus
INTIMAL HYPERPLASIA
Iliac Artery/pathology
PROLIFERATION
Polymers
RESTENOSIS
Rabbits
Stents
Tretinoin/pharmacology

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Cite this

Samara, I., Katsouras, C. S., Semertzioglou, A., Vratimos, A., Moula, A. I., Dimitriou, C. A., Theofanis, M., Papadimitropoulou, T., Bouratzis, V., Karanasiou, G., Fotiadis, D., Michalis, L. K., & Moulas, A. N. (2022). Histopathological evaluation of a retinoic acid eluting stent in a rabbit iliac artery model. Scientific Reports, 12(1), Article 13305. https://doi.org/10.1038/s41598-022-16025-5

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abstract = "This study aimed to evaluate the safety and efficacy of innovative retinoic acid (RA) eluting stents with bioabsorbable polymer. Sixty stents divided in ten groups were implanted in the iliac arteries of 30 rabbits. Two polymers ({"}A{"}, poly (lactic-co-glycolic acid) and {"}B{"}, polylactic acid), and three doses ({"}Low{"}, {"}Medium{"} and {"}High{"}) of RA (groups: AL, AM, AH, BL, BM, BH) were used on cobalt chromium stents (Rontis Corporation), one group of bare stent (C), one group (D) of Everolimus eluting stent (Xience-Pro, Abbot Vascular), and two groups of Rontis Everolimus eluting stents coated with polymer A (EA) and B (EB). Treated arteries were explanted after 4 weeks, processed by methyl methacrylate resin and evaluated by histopathology. None of the implanted stents was related with thrombus formation or extensive inflammation. Image analysis showed limited differences between groups regarding area stenosis (BH, D and EB groups had the lower values). Group BH had lower intimal mean thickness than AH (105.1 vs 75.3 μm, p = 0.024). Stents eluting RA, a non-cytotoxic drug, were not related with thrombus formation and had an acceptable degree of stenosis 4 weeks post implantation. RA dose and type of polymer may play role in the biocompatibility of the stents.",

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author = "Ioanna Samara and Katsouras, {Christos S} and Arsen Semertzioglou and Athanassios Vratimos and Moula, {Amalia I} and Dimitriou, {Constantinos A} and Michail Theofanis and Triantafyllia Papadimitropoulou and Vasileios Bouratzis and Georgia Karanasiou and Dimitrios Fotiadis and Michalis, {Lampros K} and Moulas, {Anargyros N}",

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Samara, I, Katsouras, CS, Semertzioglou, A, Vratimos, A, Moula, AI, Dimitriou, CA, Theofanis, M, Papadimitropoulou, T, Bouratzis, V, Karanasiou, G, Fotiadis, D, Michalis, LK & Moulas, AN 2022, 'Histopathological evaluation of a retinoic acid eluting stent in a rabbit iliac artery model', Scientific Reports, vol. 12, no. 1, 13305. https://doi.org/10.1038/s41598-022-16025-5

TY - JOUR

T1 - Histopathological evaluation of a retinoic acid eluting stent in a rabbit iliac artery model

AU - Samara, Ioanna

AU - Katsouras, Christos S

AU - Semertzioglou, Arsen

AU - Vratimos, Athanassios

AU - Moula, Amalia I

AU - Dimitriou, Constantinos A

AU - Theofanis, Michail

AU - Papadimitropoulou, Triantafyllia

AU - Bouratzis, Vasileios

AU - Karanasiou, Georgia

AU - Fotiadis, Dimitrios

AU - Michalis, Lampros K

AU - Moulas, Anargyros N

PY - 2022/8/3

Y1 - 2022/8/3

N2 - This study aimed to evaluate the safety and efficacy of innovative retinoic acid (RA) eluting stents with bioabsorbable polymer. Sixty stents divided in ten groups were implanted in the iliac arteries of 30 rabbits. Two polymers ("A", poly (lactic-co-glycolic acid) and "B", polylactic acid), and three doses ("Low", "Medium" and "High") of RA (groups: AL, AM, AH, BL, BM, BH) were used on cobalt chromium stents (Rontis Corporation), one group of bare stent (C), one group (D) of Everolimus eluting stent (Xience-Pro, Abbot Vascular), and two groups of Rontis Everolimus eluting stents coated with polymer A (EA) and B (EB). Treated arteries were explanted after 4 weeks, processed by methyl methacrylate resin and evaluated by histopathology. None of the implanted stents was related with thrombus formation or extensive inflammation. Image analysis showed limited differences between groups regarding area stenosis (BH, D and EB groups had the lower values). Group BH had lower intimal mean thickness than AH (105.1 vs 75.3 μm, p = 0.024). Stents eluting RA, a non-cytotoxic drug, were not related with thrombus formation and had an acceptable degree of stenosis 4 weeks post implantation. RA dose and type of polymer may play role in the biocompatibility of the stents.

AB - This study aimed to evaluate the safety and efficacy of innovative retinoic acid (RA) eluting stents with bioabsorbable polymer. Sixty stents divided in ten groups were implanted in the iliac arteries of 30 rabbits. Two polymers ("A", poly (lactic-co-glycolic acid) and "B", polylactic acid), and three doses ("Low", "Medium" and "High") of RA (groups: AL, AM, AH, BL, BM, BH) were used on cobalt chromium stents (Rontis Corporation), one group of bare stent (C), one group (D) of Everolimus eluting stent (Xience-Pro, Abbot Vascular), and two groups of Rontis Everolimus eluting stents coated with polymer A (EA) and B (EB). Treated arteries were explanted after 4 weeks, processed by methyl methacrylate resin and evaluated by histopathology. None of the implanted stents was related with thrombus formation or extensive inflammation. Image analysis showed limited differences between groups regarding area stenosis (BH, D and EB groups had the lower values). Group BH had lower intimal mean thickness than AH (105.1 vs 75.3 μm, p = 0.024). Stents eluting RA, a non-cytotoxic drug, were not related with thrombus formation and had an acceptable degree of stenosis 4 weeks post implantation. RA dose and type of polymer may play role in the biocompatibility of the stents.

KW - Animals

KW - Constriction, Pathologic/pathology

KW - Drug-Eluting Stents

KW - Everolimus

KW - INTIMAL HYPERPLASIA

KW - Iliac Artery/pathology

KW - PROLIFERATION

KW - Polymers

KW - RESTENOSIS

KW - Rabbits

KW - Stents

KW - Tretinoin/pharmacology

U2 - 10.1038/s41598-022-16025-5

DO - 10.1038/s41598-022-16025-5

M3 - Article

C2 - 35922518

SN - 2045-2322

VL - 12

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 13305

ER -