Functionalized screen-printed electrodes for the thermal detection of Escherichia coli in dairy products

Rocio Arreguin-Campos; Margaux Frigoli; Manlio Caldara; Robert D Crapnell; Alejandro Garcia-Miranda Ferrari; Craig E Banks; Thomas J Cleij; Hanne Diliën; Kasper Eersels; Bart van Grinsven

doi:10.1016/j.foodchem.2022.134653

Functionalized screen-printed electrodes for the thermal detection of Escherichia coli in dairy products

Rocio Arreguin-Campos^*, Margaux Frigoli, Manlio Caldara, Robert D Crapnell, Alejandro Garcia-Miranda Ferrari, Craig E Banks, Thomas J Cleij, Hanne Diliën, Kasper Eersels, Bart van Grinsven

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Accurate and fast on-site detection of harmful microorganisms in food products is a key preventive step to avoid food-borne illness and product recall. In this study, screen-printed electrodes (SPEs) were functionalized via a facile strategy with surface imprinted polymers (SIPs). The SIP-coated SPEs were used in combination with the heat transfer method (HTM) for the real-time detection of Escherichia coli. The sensor was tested in buffer, with a reproducible and sensitive response that attained a limit of detection of 180 CFU/mL. Furthermore, selectivity was assessed by analyzing the sensor's response to C. sakazakii, K. pneumoniae and S. aureus as analogue strains. Finally, the device was successfully used for the detection of E. coli in spiked milk as proof-of-application, requiring no additional sample preparation. These results suggest the proposed thermal biosensor possesses the potential of becoming a tool for routine, on-site monitoring of E. coli in food safety applications.

Original language	English
Article number	134653
Number of pages	7
Journal	Food Chemistry
Volume	404
Issue number	Pt B
Early online date	31 Oct 2022
DOIs	https://doi.org/10.1016/j.foodchem.2022.134653
Publication status	Published - 15 Mar 2023

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Cite this

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title = "Functionalized screen-printed electrodes for the thermal detection of Escherichia coli in dairy products",

abstract = "Accurate and fast on-site detection of harmful microorganisms in food products is a key preventive step to avoid food-borne illness and product recall. In this study, screen-printed electrodes (SPEs) were functionalized via a facile strategy with surface imprinted polymers (SIPs). The SIP-coated SPEs were used in combination with the heat transfer method (HTM) for the real-time detection of Escherichia coli. The sensor was tested in buffer, with a reproducible and sensitive response that attained a limit of detection of 180 CFU/mL. Furthermore, selectivity was assessed by analyzing the sensor's response to C. sakazakii, K. pneumoniae and S. aureus as analogue strains. Finally, the device was successfully used for the detection of E. coli in spiked milk as proof-of-application, requiring no additional sample preparation. These results suggest the proposed thermal biosensor possesses the potential of becoming a tool for routine, on-site monitoring of E. coli in food safety applications.",

author = "Rocio Arreguin-Campos and Margaux Frigoli and Manlio Caldara and Crapnell, {Robert D} and Ferrari, {Alejandro Garcia-Miranda} and Banks, {Craig E} and Cleij, {Thomas J} and Hanne Dili{\"e}n and Kasper Eersels and {van Grinsven}, Bart",

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doi = "10.1016/j.foodchem.2022.134653",

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T1 - Functionalized screen-printed electrodes for the thermal detection of Escherichia coli in dairy products

AU - Arreguin-Campos, Rocio

AU - Frigoli, Margaux

AU - Caldara, Manlio

AU - Crapnell, Robert D

AU - Ferrari, Alejandro Garcia-Miranda

AU - Banks, Craig E

AU - Cleij, Thomas J

AU - Diliën, Hanne

AU - Eersels, Kasper

AU - van Grinsven, Bart

PY - 2023/3/15

Y1 - 2023/3/15

N2 - Accurate and fast on-site detection of harmful microorganisms in food products is a key preventive step to avoid food-borne illness and product recall. In this study, screen-printed electrodes (SPEs) were functionalized via a facile strategy with surface imprinted polymers (SIPs). The SIP-coated SPEs were used in combination with the heat transfer method (HTM) for the real-time detection of Escherichia coli. The sensor was tested in buffer, with a reproducible and sensitive response that attained a limit of detection of 180 CFU/mL. Furthermore, selectivity was assessed by analyzing the sensor's response to C. sakazakii, K. pneumoniae and S. aureus as analogue strains. Finally, the device was successfully used for the detection of E. coli in spiked milk as proof-of-application, requiring no additional sample preparation. These results suggest the proposed thermal biosensor possesses the potential of becoming a tool for routine, on-site monitoring of E. coli in food safety applications.

AB - Accurate and fast on-site detection of harmful microorganisms in food products is a key preventive step to avoid food-borne illness and product recall. In this study, screen-printed electrodes (SPEs) were functionalized via a facile strategy with surface imprinted polymers (SIPs). The SIP-coated SPEs were used in combination with the heat transfer method (HTM) for the real-time detection of Escherichia coli. The sensor was tested in buffer, with a reproducible and sensitive response that attained a limit of detection of 180 CFU/mL. Furthermore, selectivity was assessed by analyzing the sensor's response to C. sakazakii, K. pneumoniae and S. aureus as analogue strains. Finally, the device was successfully used for the detection of E. coli in spiked milk as proof-of-application, requiring no additional sample preparation. These results suggest the proposed thermal biosensor possesses the potential of becoming a tool for routine, on-site monitoring of E. coli in food safety applications.

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DO - 10.1016/j.foodchem.2022.134653

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