FTICR mass spectrometry-based multivariate analysis to explore distinctive metabolites and metabolic pathways: A comprehensive bioanalytical strategy toward time-course metabolic profiling of Thymus vulgaris plants responding to drought stress

Mohammad Shahbazy, Parviz Moradi, Gokhan Ertaylan, Ali Zahraei, Mohsen Kompany-Zareh

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In this research, metabolic profiling/pathways of Thymus vulgaris (thyme) plant were assessed during a water deficit stress using an FTICR mass spectrometry-based metabolomics strategy incorporating multivariate data analysis and bioinformatics techniques. Herein, differences of MS signals in specific time courses after water deficit stress and control cases without any timing period were distinguished significantly by common pattern recognition techniques, i.e., PCA, HCA-Heatmap, and PLS-DA. Subsequently, the results were compared with supervised Kohonen neural network (SKN) ones as a non-linear data visualization and capable mapping tool. The classification models showed excellent performance to predict the level of drought stress. By assessing variances contribution on the PCA-loadings of the MS data, the discriminant variables related to the most critical metabolites were identified and then confirmed by ANOVA. Indeed, FTICR MS-based multivariate analysis strategy could explore distinctive metabolites and metabolic pathways/profiles, grouped into three metabolism categories including amino acids, carbohydrates (i.e., galactose, glucose, fructose, sucrose, and mannose), and other metabolites (rosmarinic acid and citrate), to indicate biological mechanisms in response to drought stress for thyme. It was achieved and approved through the MS signals, genomics databases, and transcriptomics factors to interpret and predict the plant metabolic behavior. Eventually, a comprehensive pathway analysis was used to provide a pathway enrichment analysis and explore topological pathway characteristics dealing with the remarkable metabolites to demonstrate that galactose metabolism is the most significant pathway in the biological system of thyme
Original languageEnglish
Article number110257
Number of pages14
JournalPlant Science
Volume290
DOIs
Publication statusPublished - Jan 2020

Keywords

  • Thymus vulgaris plant
  • Water deficit stress
  • PTICR mass spectrometry
  • Metabolomics
  • Metabolic pathways
  • Multivariate data analysis
  • DEHYDRATION TOLERANCE
  • ANTIOXIDANT CAPACITY
  • KYOTO ENCYCLOPEDIA
  • LIPID-COMPOSITION
  • MOLECULAR-BASIS
  • WATER-STRESS
  • NETWORKS
  • ARABIDOPSIS
  • TEMPERATURE
  • RESPONSES

Cite this