TY - JOUR
T1 - Fabrication and characterization smart gold-polymer nanostructure as promising theranostic agent for dual-imaging and chemo-photothermal therapy of cancer
T2 - An in vitro study
AU - Azizi, Mehdi
AU - Pakravan, Asrin
AU - Valizadeh, Hadi
AU - Rahbarghazi, Reza
AU - Dianat-Moghadam, Hassan
AU - Bani, Farhad
AU - Kahroba, Houman
AU - Salehi, Roya
AU - Mehrmohammadi, Mohammad
N1 - Funding Information:
This study was financially supported by grant no. 59950 from Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran.
Publisher Copyright:
© 2024 Elsevier B.V.
PY - 2024/5/1
Y1 - 2024/5/1
N2 - In order to enhance the effectiveness of cancer therapy, it is essential to have highly sensitive imaging techniques that provide accurate results and the selection of appropriate therapeutic strategies. Despite recent advances in technologies associated with tumor imaging, the application of conventional single-mode imaging is the subject of debate. Herein, we strategically engineered a nanostructure (GNSs-MTX@CD-Pol) designed from a pH-responsive polymer (Pol), gold nanostar (GNSs), carbon dot (CD), and methotrexate (MTX) as a theranostic agent for simultaneously fluorescent (FI) and X-ray computed tomography (CT) imaging and combination Chemo-Photothermal Therapy in response to near-infrared (NIR) laser irradiation. GNSs-MTX@CD-Pol NPs possess excellent photothermal conversion efficiency and dual FI/CT imaging properties, which are attributed to the strong NIR absorption and high atomic number of gold elements. Moreover, it is demonstrated that GNSs-MTX@CD-Pol NPs are effectively responding to tumor acidity. With the PTT, the growth of cancer cells can be remarkably ablated in vitro. The results of the cell cycle, apoptosis, real-time PCR, and western blotting test on MDA-MB-231 cells revealed antitumor effect of GNSs-MTX@CD-Pol NPs caused by combination Chemo-Photothermal Therapy. Based on their high stability and excellent biocompatibility, GNSs-MTX@CD-Pol NPs have great potential for the treatment of various types of tumors.
AB - In order to enhance the effectiveness of cancer therapy, it is essential to have highly sensitive imaging techniques that provide accurate results and the selection of appropriate therapeutic strategies. Despite recent advances in technologies associated with tumor imaging, the application of conventional single-mode imaging is the subject of debate. Herein, we strategically engineered a nanostructure (GNSs-MTX@CD-Pol) designed from a pH-responsive polymer (Pol), gold nanostar (GNSs), carbon dot (CD), and methotrexate (MTX) as a theranostic agent for simultaneously fluorescent (FI) and X-ray computed tomography (CT) imaging and combination Chemo-Photothermal Therapy in response to near-infrared (NIR) laser irradiation. GNSs-MTX@CD-Pol NPs possess excellent photothermal conversion efficiency and dual FI/CT imaging properties, which are attributed to the strong NIR absorption and high atomic number of gold elements. Moreover, it is demonstrated that GNSs-MTX@CD-Pol NPs are effectively responding to tumor acidity. With the PTT, the growth of cancer cells can be remarkably ablated in vitro. The results of the cell cycle, apoptosis, real-time PCR, and western blotting test on MDA-MB-231 cells revealed antitumor effect of GNSs-MTX@CD-Pol NPs caused by combination Chemo-Photothermal Therapy. Based on their high stability and excellent biocompatibility, GNSs-MTX@CD-Pol NPs have great potential for the treatment of various types of tumors.
KW - Chemo-Photothermal Therapy
KW - Dual-Imaging Modality
KW - Gold-Polymer Nanostructures
KW - Theranostic
KW - Tumoricidal Effects
U2 - 10.1016/j.jphotochem.2024.115459
DO - 10.1016/j.jphotochem.2024.115459
M3 - Article
SN - 1010-6030
VL - 450
JO - Journal of Photochemistry and Photobiology A-Chemistry
JF - Journal of Photochemistry and Photobiology A-Chemistry
M1 - 115459
ER -