TY - JOUR
T1 - Ex Vivo and In Vivo Administration of Fluorescent CNA35 Specifically Marks Cardiac Fibrosis
AU - de Jong, Sanne
AU - van Middendorp, Lars B.
AU - Hermans, Robin H. A.
AU - de Bakker, Jacques M. T.
AU - Bierhuizen, Marti F. A.
AU - Prinzen, Frits W.
AU - van Rijen, Harold V. M.
AU - Losen, Mario
AU - Vos, Marc A.
AU - van Zandvoort, Marc A. M. J.
PY - 2014/12
Y1 - 2014/12
N2 - Cardiac fibrosis is a major hallmark of cardiac diseases. For evaluation of cardiac fibrosis, the development of highly specific and preferably noninvasive methods is desired. Our aim was to evaluate CNA35, a protein known to specifically bind to collagen, as a specific marker of cardiac fibrosis. Fluorescently labeled CNA35 was applied ex vivo on tissue sections of fibrotic rat, mouse, and canine myocardium. After quantification of CNA35, sections were examined with picrosirius red (PSR) and compared to CNA35. Furthermore, fluorescently labeled CNA35 was administered in vivo in mice. Hearts were isolated, and CNA35 labeling was examined in tissue sections. Serial sections were histologically examined with PSR. Ex vivo application of CNA35 showed specific binding to collagen and a high correlation with PSR (Pearson r = .86 for mice/rats and r = .98 for canine; both p,
AB - Cardiac fibrosis is a major hallmark of cardiac diseases. For evaluation of cardiac fibrosis, the development of highly specific and preferably noninvasive methods is desired. Our aim was to evaluate CNA35, a protein known to specifically bind to collagen, as a specific marker of cardiac fibrosis. Fluorescently labeled CNA35 was applied ex vivo on tissue sections of fibrotic rat, mouse, and canine myocardium. After quantification of CNA35, sections were examined with picrosirius red (PSR) and compared to CNA35. Furthermore, fluorescently labeled CNA35 was administered in vivo in mice. Hearts were isolated, and CNA35 labeling was examined in tissue sections. Serial sections were histologically examined with PSR. Ex vivo application of CNA35 showed specific binding to collagen and a high correlation with PSR (Pearson r = .86 for mice/rats and r = .98 for canine; both p,
U2 - 10.2310/7290.2014.00036
DO - 10.2310/7290.2014.00036
M3 - Article
C2 - 25249247
SN - 1535-3508
VL - 13
JO - Molecular Imaging
JF - Molecular Imaging
IS - 10
ER -