Evaluation of a real-time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm neonates with suspected late-onset sepsis

Marre van den Brand; Frank A. M. van den Dungen; Martine P. Bos; Mirjam M. van Weissenbruch; A. Marceline van Furth; Annemieke de Lange; Anna Rubenjan; Remco P. H. Peters; Paul H. M. Savelkoul

doi:10.1186/s13054-018-2010-4

Evaluation of a real-time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm neonates with suspected late-onset sepsis

Marre van den Brand^*, Frank A. M. van den Dungen, Martine P. Bos, Mirjam M. van Weissenbruch, A. Marceline van Furth, Annemieke de Lange, Anna Rubenjan, Remco P. H. Peters, Paul H. M. Savelkoul

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Original language	English
Article number	105
Number of pages	10
Journal	Critical Care
Volume	22
DOIs	https://doi.org/10.1186/s13054-018-2010-4
Publication status	Published - 22 Apr 2018

Keywords

Molecular diagnosis
Late-onset sepsis
Neonatology
Real-time PCR
Bacterial DNA load
Bacteremia
POLYMERASE-CHAIN-REACTION
BIRTH-WEIGHT INFANTS
STREAM INFECTIONS
RAPID DIAGNOSIS
NECROTIZING ENTEROCOLITIS
MOLECULAR-DETECTION
METAANALYSIS
CHILDREN
DISEASE
LOAD

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10.1186/s13054-018-2010-4

https://doi.org/10.1186/s13054-018-2010-4

Cite this

van den Brand, M., van den Dungen, F. A. M., Bos, M. P., van Weissenbruch, M. M., van Furth, A. M., de Lange, A., Rubenjan, A., Peters, R. P. H., & Savelkoul, P. H. M. (2018). Evaluation of a real-time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm neonates with suspected late-onset sepsis. Critical Care, 22, [105]. https://doi.org/10.1186/s13054-018-2010-4

van den Brand, Marre ; van den Dungen, Frank A. M. ; Bos, Martine P. ; van Weissenbruch, Mirjam M. ; van Furth, A. Marceline ; de Lange, Annemieke ; Rubenjan, Anna ; Peters, Remco P. H. ; Savelkoul, Paul H. M. / Evaluation of a real-time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm neonates with suspected late-onset sepsis. In: Critical Care. 2018 ; Vol. 22.

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title = "Evaluation of a real-time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm neonates with suspected late-onset sepsis",

keywords = "Molecular diagnosis, Late-onset sepsis, Neonatology, Real-time PCR, Bacterial DNA load, Bacteremia, POLYMERASE-CHAIN-REACTION, BIRTH-WEIGHT INFANTS, STREAM INFECTIONS, RAPID DIAGNOSIS, NECROTIZING ENTEROCOLITIS, MOLECULAR-DETECTION, METAANALYSIS, CHILDREN, DISEASE, LOAD",

author = "{van den Brand}, Marre and {van den Dungen}, {Frank A. M.} and Bos, {Martine P.} and {van Weissenbruch}, {Mirjam M.} and {van Furth}, {A. Marceline} and {de Lange}, Annemieke and Anna Rubenjan and Peters, {Remco P. H.} and Savelkoul, {Paul H. M.}",

year = "2018",

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day = "22",

doi = "10.1186/s13054-018-2010-4",

language = "English",

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van den Brand, M, van den Dungen, FAM, Bos, MP, van Weissenbruch, MM, van Furth, AM, de Lange, A, Rubenjan, A, Peters, RPH & Savelkoul, PHM 2018, 'Evaluation of a real-time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm neonates with suspected late-onset sepsis', Critical Care, vol. 22, 105. https://doi.org/10.1186/s13054-018-2010-4

Evaluation of a real-time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm neonates with suspected late-onset sepsis. / van den Brand, Marre; van den Dungen, Frank A. M.; Bos, Martine P.; van Weissenbruch, Mirjam M.; van Furth, A. Marceline; de Lange, Annemieke; Rubenjan, Anna; Peters, Remco P. H.; Savelkoul, Paul H. M.

In: Critical Care, Vol. 22, 105, 22.04.2018.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Evaluation of a real-time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm neonates with suspected late-onset sepsis

AU - van den Brand, Marre

AU - van den Dungen, Frank A. M.

AU - Bos, Martine P.

AU - van Weissenbruch, Mirjam M.

AU - van Furth, A. Marceline

AU - de Lange, Annemieke

AU - Rubenjan, Anna

AU - Peters, Remco P. H.

AU - Savelkoul, Paul H. M.

PY - 2018/4/22

Y1 - 2018/4/22

KW - Molecular diagnosis

KW - Late-onset sepsis

KW - Neonatology

KW - Real-time PCR

KW - Bacterial DNA load

KW - Bacteremia

KW - POLYMERASE-CHAIN-REACTION

KW - BIRTH-WEIGHT INFANTS

KW - STREAM INFECTIONS

KW - RAPID DIAGNOSIS

KW - NECROTIZING ENTEROCOLITIS

KW - MOLECULAR-DETECTION

KW - METAANALYSIS

KW - CHILDREN

KW - DISEASE

KW - LOAD

U2 - 10.1186/s13054-018-2010-4

DO - 10.1186/s13054-018-2010-4

M3 - Article

C2 - 29679983

VL - 22

JO - Critical Care

JF - Critical Care

SN - 1364-8535

M1 - 105

ER -