@article{dc87b17e32f549329be60e3545bd7e90,
title = "EndOxy: Dynamic Long-Term Evaluation of Endothelialized Gas Exchange Membranes for a Biohybrid Lung",
abstract = "In the concept of a biohybrid lung, endothelial cells seeded on gas exchange membranes form a non-thrombogenic an anti-inflammatory surface to overcome the lacking hemocompatibility of today's oxygenators during extracorporeal membrane oxygenation. To evaluate this concept, the long-term stability and gas exchange performance of endothelialized RGD-conjugated polydimethylsiloxane (RGD-PDMS) membranes was evaluated. Human umbilical vein endothelial cells (ECs) were cultured on RGD-PDMS in a model system under physiological wall shear stress (WSS) of 0.5 Pa for up to 33 days. Gas exchange performance was tested with three biological replicates under elevated WSS of 2.5 Pa using porcine blood adjusted to venous values following ISO 7199 and blood gas analysis. EC morphology was assessed by immunocytochemistry (n = 3). RGD-PDMS promoted endothelialization and stability of endothelialized membranes was shown for at least 33 days and for a maximal WSS of 2.5 Pa. Short-term exposure to porcine blood did not affect EC integrity. The gas transfer tests provided evidence for the oxygenation and decarboxylation of the blood across endothelialized membranes with a decrease of transfer rates over time that needs to be addressed in further studies with larger sample sizes. Our results demonstrate the general suitability of RGD-PDMS for biohybrid lung applications, which might enable long-term support of patients with chronic lung failure in the future.",
keywords = "Artificial lung, Extracorporeal membrane oxygenation, Gas transfer, Tissue engineering, Whole blood, VON-WILLEBRAND-FACTOR, CELLS, SECRETION, ADHESION",
author = "Sarah Klein and Felix Hesselmann and Suzana Djeljadini and Tanja Berger and Thiebes, {Anja Lena} and Thomas Schmitz-Rode and Stefan Jockenhoevel and Cornelissen, {Christian G.}",
note = "Funding Information: The authors acknowledge funding provided by the Interdisciplinary Centre for Clinical Research within the faculty of Medicine at RWTH Aachen University (T12) and the Excellence Initiative of the German federal and state governments in the framework of the i3 tm funding program. The authors thank Sophia Halbe, Jacqueline Rolfes, Caroline Kniebs and Mario Klein (Institute of Applied Medical Engineering) for assistance during gas transfer tests. The authors thank Ilona Mager and Judith Maas (Institute of Applied Medical Engineering) as well as Peter Heidenreich and Benjamin Gro{\ss}mann (ac.biomed GmbH) for providing heparinized porcine blood and for technical assistance. We thank Dr. Richard M. Twyman for professional editing of the manuscript. No benefits in any form have been or will be received from a commercial party related directly or indirectly to the subject of this manuscript. Funding Information: The authors acknowledge funding provided by the Interdisciplinary Centre for Clinical Research within the faculty of Medicine at RWTH Aachen University (T12) and the Excellence Initiative of the German federal and state governments in the framework of the itm funding program. The authors thank Sophia Halbe, Jacqueline Rolfes, Caroline Kniebs and Mario Klein (Institute of Applied Medical Engineering) for assistance during gas transfer tests. The authors thank Ilona Mager and Judith Maas (Institute of Applied Medical Engineering) as well as Peter Heidenreich and Benjamin Gro{\ss}mann (ac.biomed GmbH) for providing heparinized porcine blood and for technical assistance. We thank Dr. Richard M. Twyman for professional editing of the manuscript. 3 Publisher Copyright: {\textcopyright} 2019, The Author(s).",
year = "2020",
month = feb,
doi = "10.1007/s10439-019-02401-2",
language = "English",
volume = "48",
pages = "747--756",
journal = "Annals of Biomedical Engineering",
issn = "0090-6964",
publisher = "Springer, Cham",
number = "2",
}