Effects of a Hypercaloric and Hypocaloric Diet on Insulin-Induced Microvascular Recruitment, Glucose Uptake, and Lipolysis in Healthy Lean Men

A.L. Emanuel*, R.I. Meijer, J. Woerdeman, D.H. van Raalte, M. Diamant, M.H.H. Kramer, M.J. Serlie, E.C. Eringa, E.H. Serne

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Web of Science)

Abstract

OBJECTIVE: In mice fed a high-fat diet, impairment of insulin signaling in endothelium is an early phenomenon that precedes decreased insulin sensitivity of skeletal muscle, adipose tissue, and liver. We assessed in humans whether short-term overfeeding affects insulin-induced microvascular recruitment in skeletal muscle and adipose tissue before changes occur in glucose uptake and lipolysis.

APPROACH AND RESULTS: Fifteen healthy males underwent a hypercaloric and subsequent hypocaloric diet intervention. Before, during, and after the hypercaloric diet, and upon return to baseline weight, all participants underwent (1) a hyperinsulinemic-euglycemic clamp to determine insulin-induced glucose uptake and suppression of lipolysis (2) contrast-enhanced ultrasonography to measure insulin-induced microvascular recruitment in skeletal muscle and adipose tissue. In addition, we assessed insulin-induced vasodilation of isolated skeletal muscle resistance arteries by pressure myography after the hypercaloric diet in study participants and controls (n=5). The hypercaloric diet increased body weight (3.5 kg; P

CONCLUSIONS: In lean men, short-term overfeeding reduces insulin-induced vasodilation of skeletal muscle resistance arteries and shifts the distribution of tissue perfusion during hyperinsulinemia from skeletal muscle to adipose tissue without affecting glucose uptake and lipolysis.

Original languageEnglish
Pages (from-to)1695-1704
Number of pages10
JournalArteriosclerosis Thrombosis and Vascular Biology
Volume40
Issue number7
DOIs
Publication statusPublished - 1 Jul 2020

Keywords

  • adipose tissue
  • adipose-tissue
  • diet
  • dysfunction
  • endothelium
  • in-vivo
  • inflammation
  • insulin resistance
  • lipolysis
  • microcirculation
  • mixed meal
  • muscle
  • obesity
  • perfusion
  • perfusion imaging
  • resistance
  • sensitivity
  • MUSCLE
  • SENSITIVITY
  • PERFUSION
  • DYSFUNCTION
  • ADIPOSE-TISSUE
  • MIXED MEAL
  • OBESITY
  • INFLAMMATION
  • IN-VIVO
  • RESISTANCE

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