TY - JOUR
T1 - Development of 4D-bioprinted shape-morphing magnetic constructs for cartilage regeneration using a silk fibroin-gelatin bioink
AU - Chakraborty, Juhi
AU - Fernández-Pérez, Julia
AU - Takhsha Ghahfarokhi, Milad
AU - van Kampen, Kenny A.
AU - ten Brink, Tim
AU - Ramis, Jopeth
AU - Kalogeropoulou, Maria
AU - Cabassi, Riccardo
AU - de Julián Fernández, César
AU - Albertini, Franca
AU - Mota, Carlos
AU - Ghosh, Sourabh
AU - Moroni, Lorenzo
N1 - Funding Information:
J.C. is grateful to the Department of Science and Technology , India, for the Inspire Fellowship no. IF160365 . We are also grateful to ZonMw (project number 11630095101) for funding. This work is also part of the project 3D-MENTOR (with project number 18647) of the VICI Research Program , financed by the Dutch Research Council (NWO).
Publisher Copyright:
© 2024 The Author(s)
PY - 2024/3/20
Y1 - 2024/3/20
N2 - External magnetic fields can regulate cellular responses when interacting with magnetic nanoparticles (MNPs). Here, we develop a 4D-bioprinted construct by incorporating anisotropic MNPs into a silk fibroin-gelatin bioink with human bone-marrow-derived mesenchymal stromal cells for cartilage regeneration. We measure the magnetic-field-induced temperature response of the acellular construct, determine its mechanical response (actuation), and compare it with the MNPs. Constructs are then magnetically actuated, and their effects on chondrogenesis are investigated. Actuation is induced cyclically every other day (5 min and 30 min/day) for 21 days. Actuation for 30 min exhibits enhanced early (Sox-9 and aggrecan) and late (collagen-II) expression with downregulation of hypertrophic markers (collagen-X and matrix metalloproteinase-13) and enhanced matrix deposition, total collagen, and glycosaminoglycan compared to the constructs actuated for 5 min, kept static, or with no MNPs. Hence, magnetic field actuation could be a significant strategy to stimulate constructs mechanically for articular cartilage regeneration.
AB - External magnetic fields can regulate cellular responses when interacting with magnetic nanoparticles (MNPs). Here, we develop a 4D-bioprinted construct by incorporating anisotropic MNPs into a silk fibroin-gelatin bioink with human bone-marrow-derived mesenchymal stromal cells for cartilage regeneration. We measure the magnetic-field-induced temperature response of the acellular construct, determine its mechanical response (actuation), and compare it with the MNPs. Constructs are then magnetically actuated, and their effects on chondrogenesis are investigated. Actuation is induced cyclically every other day (5 min and 30 min/day) for 21 days. Actuation for 30 min exhibits enhanced early (Sox-9 and aggrecan) and late (collagen-II) expression with downregulation of hypertrophic markers (collagen-X and matrix metalloproteinase-13) and enhanced matrix deposition, total collagen, and glycosaminoglycan compared to the constructs actuated for 5 min, kept static, or with no MNPs. Hence, magnetic field actuation could be a significant strategy to stimulate constructs mechanically for articular cartilage regeneration.
KW - 4D bioprinting
KW - anisotropic magnetic nanoparticles
KW - chondrogenesis
KW - magnetic actuation
KW - magnetic field
KW - magnetic hyperthermia
KW - shape morphing
KW - silk-gelatin
U2 - 10.1016/j.xcrp.2024.101819
DO - 10.1016/j.xcrp.2024.101819
M3 - Article
VL - 5
JO - Cell Reports Physical Science
JF - Cell Reports Physical Science
IS - 3
M1 - 101819
ER -