@article{980bc395b57d4f8e9bc42fb6e62f3a3d,
title = "Development and evaluation of matrix application techniques for high throughput mass spectrometry imaging of tissues in the clinic",
abstract = "Matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI) is a sensitive label-free technique that can be used to study a wide variety of clinical phenotypes. In this context, MSI offers huge diagnostic potential by supporting decision making in the determination of personalized treatment strategies. However, improvements in throughput and robustness are still needed before it finds a place in routine application. While the field has seen tremendous improvements in the throughput of data acquisition, robust and high-throughput sample preparation methods compatible with these acquisition methods need to be developed. To address this challenge, we have developed several methods to reduce the matrix application time to less than 5 min, while maintaining sensitivity and reproducibility. Workflows incorporating these methods provide a pipeline analysis time for MSI sample preparation and acquisition of less than 30 min. The reduced time for these analyses will contribute towards the integration of MSI into routine molecular pathology for clinical diagnostics.",
keywords = "HIGH-SPATIAL-RESOLUTION, MOUSE-BRAIN, LIPIDS, SUBLIMATION, PROTEINS, LOCALIZATION, TRANSMISSION, SECTIONS, TIME",
author = "Huizing, {Lennart R. S.} and Ellis, {Shane R.} and Beulen, {Bart W. A. M. M.} and Barre, {Florian P. Y.} and Kwant, {Paul B.} and Vreeken, {Rob J.} and Heeren, {Ron M. A.}",
note = "Funding Information: This research was made possible with the financial support of the LINK program of the Dutch Province of Limburg. The matrix sublimation development was supported by an Enabling Technologies B.V valorization project. F.P.Y.B. received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie Program, TargetCare (ITN-2014-ETN 642414). We gratefully acknowledge support of Janssen R&D (Beerse, Belgium).LRS Huizing has a patent Sample preparation device pending; BWAMM Beulen reports Grants from Enabling Technologies, Grants from NWO KIEM, during the conduct of the study; In addition, Dr. Beulen has a patent on matrix sublimation technology. S.R. Ellis has a patent Sample preparation device pending. FPY Barr{\'e} reports other funding from HTX Imaging, during the conduct of the study; PB Kwant reports a patent Sample Preparation Apparatus pending with royalties received from HTX Imaging BV, and a patent null pending. RJ Vreeken has nothing to disclose; RMA Heeren reports grants from Province of Limburg, during the conduct of the study; In addition, RMA. Heeren has a patent Sample preparation device pending. Funding Information: This research was made possible with the financial support of the LINK program of the Dutch Province of Limburg . The matrix sublimation development was supported by an Enabling Technologies B.V valorization project. F.P.Y.B. received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie Program, TargetCare ( ITN-2014-ETN 642414 ). We gratefully acknowledge support of Janssen R&D (Beerse, Belgium). Publisher Copyright: {\textcopyright} 2019 The Association for Mass Spectrometry: Applications to the Clinical Lab (MSACL)",
year = "2019",
month = apr,
doi = "10.1016/j.clinms.2019.01.004",
language = "English",
volume = "12",
pages = "7--15",
journal = "Clinical mass spectrometry",
issn = "2376-9998",
publisher = "Elsevier",
}