Decreased interleukin(IL)-35 Expression is Associated with Active Intraocular Inflammation in Vogt-Koyanagi-Harada (VKH) Disease

Jianping Hu; Yirong Qin; Shenglan Yi; Chaokui Wang; Jinglu Yang; Lu Yang; Luoziyi Wang; Aize Kijlstra; Peizeng Yang; Hong Li

doi:10.1080/09273948.2018.1433306

Decreased interleukin(IL)-35 Expression is Associated with Active Intraocular Inflammation in Vogt-Koyanagi-Harada (VKH) Disease

Jianping Hu, Yirong Qin, Shenglan Yi, Chaokui Wang, Jinglu Yang, Lu Yang, Luoziyi Wang, Aize Kijlstra, Peizeng Yang, Hong Li^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Purpose: Recent studies have reported that IL-35 has a protective effect in autoimmune disease. In this study, we explored the role of IL-35 in the pathogenesis of Vogt-Koyanagi-Harada (VKH) disease. Methods: The IL-35/EBI3 and IL-35/P35 mRNA level was assayed by Real-Time PCR. The level of IL-35 in serum was detected by ELISA. PBMCs and monocyte-derived DCs were cultured with or without IL-35 and the concentration of IL-17, IL-10, IFN-gamma, IL-6, TNF-alpha, and IL-1 beta in supernatants was tested by ELISA. Results: The serum level of IL-35 is reduced in active VKH patients. The mRNA expression of the two subunits IL-35/EBI3 and IL-35/P35 in PBMCs from patients with active VKH was also decreased. IL-35 significantly inhibited IFN-gamma and IL-17 expression and induced IL-10 production by PBMCs and inhibited IL-6 production by monocyte-derived DCs. Conclusion: The current study suggests that a decreased IL-35 expression may be involved in the pathogenesis of VKH disease.

Original language	English
Pages (from-to)	595-601
Number of pages	7
Journal	Ocular Immunology and Inflammation
Volume	27
Issue number	4
DOIs	https://doi.org/10.1080/09273948.2018.1433306
Publication status	Published - 19 May 2019

Keywords

IL-35
VKH disease
DCs
uceitis
TH17 CELL RESPONSES
REGULATORY B-CELLS
DENDRITIC CELLS
CYTOKINE
LYMPHOCYTES
INHIBITION
ACTIVATION
DIAGNOSIS
IMMUNE

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10.1080/09273948.2018.1433306

Cite this

@article{eacdb3258546461b910e69049caca863,

title = "Decreased interleukin(IL)-35 Expression is Associated with Active Intraocular Inflammation in Vogt-Koyanagi-Harada (VKH) Disease",

abstract = "Purpose: Recent studies have reported that IL-35 has a protective effect in autoimmune disease. In this study, we explored the role of IL-35 in the pathogenesis of Vogt-Koyanagi-Harada (VKH) disease. Methods: The IL-35/EBI3 and IL-35/P35 mRNA level was assayed by Real-Time PCR. The level of IL-35 in serum was detected by ELISA. PBMCs and monocyte-derived DCs were cultured with or without IL-35 and the concentration of IL-17, IL-10, IFN-gamma, IL-6, TNF-alpha, and IL-1 beta in supernatants was tested by ELISA. Results: The serum level of IL-35 is reduced in active VKH patients. The mRNA expression of the two subunits IL-35/EBI3 and IL-35/P35 in PBMCs from patients with active VKH was also decreased. IL-35 significantly inhibited IFN-gamma and IL-17 expression and induced IL-10 production by PBMCs and inhibited IL-6 production by monocyte-derived DCs. Conclusion: The current study suggests that a decreased IL-35 expression may be involved in the pathogenesis of VKH disease.",

keywords = "IL-35, VKH disease, DCs, uceitis, TH17 CELL RESPONSES, REGULATORY B-CELLS, DENDRITIC CELLS, CYTOKINE, LYMPHOCYTES, INHIBITION, ACTIVATION, DIAGNOSIS, IMMUNE",

author = "Jianping Hu and Yirong Qin and Shenglan Yi and Chaokui Wang and Jinglu Yang and Lu Yang and Luoziyi Wang and Aize Kijlstra and Peizeng Yang and Hong Li",

note = "Funding Information: This work was supported by the National Key R&D Program of China (2016YFC0904000; National Natural Science Foundation Project (81470622); National Natural Science Foundation Project (81770913); and The Science and Technology Project Foundation of Chongqing (cstc2013jcyjA10008). Publisher Copyright: {\textcopyright} 2019, {\textcopyright} 2019 Taylor & Francis Group, LLC.",

year = "2019",

month = may,

day = "19",

doi = "10.1080/09273948.2018.1433306",

language = "English",

volume = "27",

pages = "595--601",

journal = "Ocular Immunology and Inflammation",

issn = "0927-3948",

publisher = "Routledge/Taylor & Francis Group",

number = "4",

}

TY - JOUR

T1 - Decreased interleukin(IL)-35 Expression is Associated with Active Intraocular Inflammation in Vogt-Koyanagi-Harada (VKH) Disease

AU - Hu, Jianping

AU - Qin, Yirong

AU - Yi, Shenglan

AU - Wang, Chaokui

AU - Yang, Jinglu

AU - Yang, Lu

AU - Wang, Luoziyi

AU - Kijlstra, Aize

AU - Yang, Peizeng

AU - Li, Hong

N1 - Funding Information: This work was supported by the National Key R&D Program of China (2016YFC0904000; National Natural Science Foundation Project (81470622); National Natural Science Foundation Project (81770913); and The Science and Technology Project Foundation of Chongqing (cstc2013jcyjA10008). Publisher Copyright: © 2019, © 2019 Taylor & Francis Group, LLC.

PY - 2019/5/19

Y1 - 2019/5/19

N2 - Purpose: Recent studies have reported that IL-35 has a protective effect in autoimmune disease. In this study, we explored the role of IL-35 in the pathogenesis of Vogt-Koyanagi-Harada (VKH) disease. Methods: The IL-35/EBI3 and IL-35/P35 mRNA level was assayed by Real-Time PCR. The level of IL-35 in serum was detected by ELISA. PBMCs and monocyte-derived DCs were cultured with or without IL-35 and the concentration of IL-17, IL-10, IFN-gamma, IL-6, TNF-alpha, and IL-1 beta in supernatants was tested by ELISA. Results: The serum level of IL-35 is reduced in active VKH patients. The mRNA expression of the two subunits IL-35/EBI3 and IL-35/P35 in PBMCs from patients with active VKH was also decreased. IL-35 significantly inhibited IFN-gamma and IL-17 expression and induced IL-10 production by PBMCs and inhibited IL-6 production by monocyte-derived DCs. Conclusion: The current study suggests that a decreased IL-35 expression may be involved in the pathogenesis of VKH disease.

AB - Purpose: Recent studies have reported that IL-35 has a protective effect in autoimmune disease. In this study, we explored the role of IL-35 in the pathogenesis of Vogt-Koyanagi-Harada (VKH) disease. Methods: The IL-35/EBI3 and IL-35/P35 mRNA level was assayed by Real-Time PCR. The level of IL-35 in serum was detected by ELISA. PBMCs and monocyte-derived DCs were cultured with or without IL-35 and the concentration of IL-17, IL-10, IFN-gamma, IL-6, TNF-alpha, and IL-1 beta in supernatants was tested by ELISA. Results: The serum level of IL-35 is reduced in active VKH patients. The mRNA expression of the two subunits IL-35/EBI3 and IL-35/P35 in PBMCs from patients with active VKH was also decreased. IL-35 significantly inhibited IFN-gamma and IL-17 expression and induced IL-10 production by PBMCs and inhibited IL-6 production by monocyte-derived DCs. Conclusion: The current study suggests that a decreased IL-35 expression may be involved in the pathogenesis of VKH disease.

KW - IL-35

KW - VKH disease

KW - DCs

KW - uceitis

KW - TH17 CELL RESPONSES

KW - REGULATORY B-CELLS

KW - DENDRITIC CELLS

KW - CYTOKINE

KW - LYMPHOCYTES

KW - INHIBITION

KW - ACTIVATION

KW - DIAGNOSIS

KW - IMMUNE

U2 - 10.1080/09273948.2018.1433306

DO - 10.1080/09273948.2018.1433306

M3 - Article

C2 - 29498905

SN - 0927-3948

VL - 27

SP - 595

EP - 601

JO - Ocular Immunology and Inflammation

JF - Ocular Immunology and Inflammation

IS - 4

ER -