Decellularized Extracellular Matrices and Cardiac Differentiation: Study on Human Amniotic Fluid-Stem Cells

G. Gaggi, A. Di Credico, P. Izzicupo, S. Sancilio, M. Di Mauro, G. Iannetti, S. Dolci, G. Amabile, A. Di Baldassarre, B. Ghinassi*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Cell therapy with a variety of stem populations is increasingly being investigated as a promising regenerative strategy for cardiovascular (CV) diseases. Their combination with adequate scaffolds represents an improved therapeutic approach. Recently, several biomaterials were investigated as scaffolds for CV tissue repair, with decellularized extracellular matrices (dECMs) arousing increasing interest for cardiac tissue engineering applications. The aim of this study was to analyze whether dECMs support the cardiac differentiation of(Cardiopoietic)AF stem cells. These perinatal stem cells, which can be easily isolated without ethical or safety limitations, display a high cardiac differentiative potential. Differentiation was previously achieved by culturing them on Matrigel, but this 3D scaffold is not transplantable. The identification of a new transplantable scaffold able to support(Cardiopoietic)AF stem cell cardiac differentiation is pivotal prior to encouraging translation of in vitro studies in animal model preclinical investigations. Our data demonstrated that decellularized extracellular matrices already used in cardiac surgery (the porcine Cor(TM)PATCH and the equine MatrixPatch(TM)) can efficiently support the proliferation and cardiac differentiation of(Cardiopoietic)AF stem cells and represent a useful cellular scaffold to be transplanted with stem cells in animal hosts.
Original languageEnglish
Article number6317
Number of pages14
JournalInternational Journal of Molecular Sciences
Issue number17
Publication statusPublished - 1 Sept 2020


  • (cardiopoietic)af cells
  • amniotic fluid stem cells
  • biomaterials
  • cardiac differentiation
  • cardiac tissue engineering
  • cardiomyocyte differentiation
  • cormatrix
  • decellularized extracellular matrix
  • generation
  • heart-failure
  • in-vitro
  • induced pluripotent stem cells
  • l-type calcium channels
  • polymorphism
  • sarcomeric proteins
  • scaffolds
  • L-type calcium channels
  • (Cardiopoietic)AF cells


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