Cytoarchitectonic and chemoarchitectonic characterization of the prefrontal cortical areas in the mouse

H. J. J. M. Van De Werd, Grazyna Rajkowska, Paul Evers, Harry B. M. Uylings*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


This study describes cytoarchitectonic criteria to define the prefrontal cortical areas in the mouse brain (C57BL/6 strain). Currently, well-illustrated mouse brain stereotaxic atlases are available, which, however, do not provide a description of the distinctive cytoarchitectonic characteristics of individual prefrontal areas. Such a description is of importance for stereological, neuronal tracing, and physiological, molecular and neuroimaging studies in which a precise parcellation of the prefrontal cortex (PFC) is required. The present study describes and illustrates: the medial prefrontal areas, i.e., the infralimbic, prelimbic, dorsal and ventral anterior cingulate and Fr2 area; areas of the lateral PFC, i.e., the dorsal agranular insular cortical areas and areas of the ventral PFC, i.e., the lateral, ventrolateral, ventral and medial orbital areas. Each cytoarchitectonically defined boundary is corroborated by one or more chemoarchitectonic stainings, i.e., acetylcholine esterase, SMI32, SMI311, dopamine, parvalbumin, calbindin and myelin staining.
Original languageEnglish
Pages (from-to)339-353
JournalBrain Structure & Function
Issue number4
Publication statusPublished - May 2010


  • Cortical parcellation
  • Infralimbic, prelimbic, anterior cingulate, Fr2, agranular insular and orbital cortical areas
  • Nissl
  • Myelin
  • Acetylcholinesterase
  • Dopamine
  • Calcium binding proteins
  • SMI-32
  • SMI-311


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