TY - JOUR
T1 - Comparison of different immunoassays for the detection of antibodies against Intrinsic Factor and Parietal Cells
AU - Lukens, Michael V.
AU - Koelman, Carin A.
AU - Curvers, Joyce
AU - Roozendaal, Caroline
AU - Bakker-Jonges, Liesbeth E.
AU - Damoiseaux, Jan G. M. C.
AU - Kroesen, Bart-Jan
N1 - Funding Information:
We gratefully acknowledge the companies D-Tek, Euroimmun, INOVA Diagnostics, Orgentec Diagnostika and ThermoFisher Scientific for providing kits and reagents. We thankfully acknowledge Dr. Christina Seebode and Dr. Kai Fechner (Euroimmun, Lübeck, Germany) for performing and evaluating the IIF analyses with the Euroimmun IIF kits for anti-IF and anti-PC antibodies and Jetske Anema, Hermien van der Meer and Petra Custers for technical assistance. We like to thank Dr. Liesbeth Brouwer for providing serum samples from healthy elderly.
Publisher Copyright:
© 2020 The Author(s)
PY - 2020/12
Y1 - 2020/12
N2 - Objectives: In the diagnostic work up of autoimmune gastritis several immunological methods are available for the detection of antibodies against Intrinsic Factor (IF) and Parietal Cells (PC). However, there are no recent reports directly comparing all the available assays and methods. The objective of this study was to compare the performance of several commercially available anti-IF and anti-PC antibody assays from different manufacturers in a multi-center multi-cohort setting.Methods: Sera were used from 5 different cohorts consisting of samples from 25 healthy elderly, 20 HCV or HIV positive patients and 150 patients positive for anti-IF or anti-PC antibodies or in whom these antibodies were requested. These cohorts were tested for anti-IF antibodies with 6 different assays (IIF, ELISA, DIA and EliA) and for anti-PC antibodies with 7 different assays (IIF, ELISA, DIA and EliA). Performance was evaluated by calculating the concordance and relative sensitivity and specificity.Results: Good concordance was found between the assays for both antibody specificities, ranging from 81 to 100% and 91-100% for anti-IF and anti-PC antibodies, respectively. Highest relative sensitivity was found with the (automated) ELISA based methods. However, all assays had a relative sensitivity between 85 and 100% for anti-IF antibodies and between 95 and 100% for anti-PC antibodies. The relative specificity ranged between 76 and 100% for anti-IF antibodies and between 96 and 100% for anti-PC antibodies.Conclusions: We conclude that most assays perform well and are concordant to each other, despite the methodological differences and the different sources of antigen used. However, the method used affects the sensitivity and specificity. The (automated) ELISA based assays have the highest relative sensitivity and relative specificity. Care should be taken in the interpretation of positive results by IIF and negative results by the Blue Diver when testing for anti-IF antibodies.
AB - Objectives: In the diagnostic work up of autoimmune gastritis several immunological methods are available for the detection of antibodies against Intrinsic Factor (IF) and Parietal Cells (PC). However, there are no recent reports directly comparing all the available assays and methods. The objective of this study was to compare the performance of several commercially available anti-IF and anti-PC antibody assays from different manufacturers in a multi-center multi-cohort setting.Methods: Sera were used from 5 different cohorts consisting of samples from 25 healthy elderly, 20 HCV or HIV positive patients and 150 patients positive for anti-IF or anti-PC antibodies or in whom these antibodies were requested. These cohorts were tested for anti-IF antibodies with 6 different assays (IIF, ELISA, DIA and EliA) and for anti-PC antibodies with 7 different assays (IIF, ELISA, DIA and EliA). Performance was evaluated by calculating the concordance and relative sensitivity and specificity.Results: Good concordance was found between the assays for both antibody specificities, ranging from 81 to 100% and 91-100% for anti-IF and anti-PC antibodies, respectively. Highest relative sensitivity was found with the (automated) ELISA based methods. However, all assays had a relative sensitivity between 85 and 100% for anti-IF antibodies and between 95 and 100% for anti-PC antibodies. The relative specificity ranged between 76 and 100% for anti-IF antibodies and between 96 and 100% for anti-PC antibodies.Conclusions: We conclude that most assays perform well and are concordant to each other, despite the methodological differences and the different sources of antigen used. However, the method used affects the sensitivity and specificity. The (automated) ELISA based assays have the highest relative sensitivity and relative specificity. Care should be taken in the interpretation of positive results by IIF and negative results by the Blue Diver when testing for anti-IF antibodies.
KW - Intrinsic Factor antibodies
KW - Parietal Cell antibodies
KW - Auto antibodies
KW - Auto immune gastritis
KW - Diagnostic laboratory methods
KW - ATROPHIC GASTRITIS
KW - ELISA
U2 - 10.1016/j.jim.2020.112867
DO - 10.1016/j.jim.2020.112867
M3 - Article
C2 - 32941886
SN - 0022-1759
VL - 487
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
M1 - 112867
ER -