Abstract
An assembled cDNA coding for the putative single-subunit NADH dehydrogenase (NDX) of Ciona intestinalis was introduced into Drosophila melanogaster. The encoded protein was found to localize to mitochondria and to confer rotenone-insensitive substrate oxidation in organello. Transgenic flies exhibited increased resistance to menadione, starvation and temperature stress, and manifested a sex and diet-dependent increase in mean lifespan of 20-50%. However, NDX was able only weakly to complement the phenotypes produced by the knockdown of complex I subunits. Published by Elsevier B.V.
Original language | English |
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Pages (from-to) | 1861-1869 |
Journal | Biochimica et Biophysica Acta-bioenergetics |
Volume | 1837 |
Issue number | 11 |
DOIs | |
Publication status | Published - Nov 2014 |
Keywords
- Alternative respiratory chain enzymes
- Tunicate
- Gene therapy
- Oxidative stress
- Ageing