Ciona intestinalis NADH dehydrogenase NDX confers stress-resistance and extended lifespan on Drosophila

Dmytro V. Gospodaryov, Oleh V. Lushchak, Bohdana M. Rovenko, Natalia V. Perkhulyn, Mike Gerards, Tea Tuomela, Howard T. Jacobs*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Web of Science)

Abstract

An assembled cDNA coding for the putative single-subunit NADH dehydrogenase (NDX) of Ciona intestinalis was introduced into Drosophila melanogaster. The encoded protein was found to localize to mitochondria and to confer rotenone-insensitive substrate oxidation in organello. Transgenic flies exhibited increased resistance to menadione, starvation and temperature stress, and manifested a sex and diet-dependent increase in mean lifespan of 20-50%. However, NDX was able only weakly to complement the phenotypes produced by the knockdown of complex I subunits. Published by Elsevier B.V.
Original languageEnglish
Pages (from-to)1861-1869
JournalBiochimica et Biophysica Acta-bioenergetics
Volume1837
Issue number11
DOIs
Publication statusPublished - Nov 2014

Keywords

  • Alternative respiratory chain enzymes
  • Tunicate
  • Gene therapy
  • Oxidative stress
  • Ageing

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